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Antioxidant activity and quality characteristics on the maturation period of the soy sauce with Gastrodia elata and oak mushroom (Lentinus edodes)

천마 및 표고버섯 첨가 전통간장의 숙성기간별 품질특성 및 항산화 활성

  • Kwon, Hyuk-Jin (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Kim, Hwa-Sun (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Choi, Yoon-Hee (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Choi, Ji-Ho (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Choi, Hae-Sun (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Song, Jin (Fermented Food Science Division, National Academy of Agricultural Science, RDA) ;
  • Park, Shin-Young (Fermented Food Science Division, National Academy of Agricultural Science, RDA)
  • 권혁진 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 김화선 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 최윤희 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 최지호 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 최혜선 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 송진 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과) ;
  • 박신영 (농촌진흥청 국립농업과학원 농식품자원부 발효식품과)
  • Received : 2014.01.02
  • Accepted : 2014.03.04
  • Published : 2014.04.30

Abstract

This study was performed to investigate the changes in antioxidant activity and quality characteristics of soy sauce with Gastrodia elata (GK) and oak mushroom (MK) in maturation period to develop a new Kanjang product with improved functional and sensory characteristics. The pH was decreased, and the total acidity was higher in GK at 25-day maturation period. Although the total sugar content increased at 15 days with GK, it was reduced after 20 days. The reducing sugar, however, showed no notable differences after 20 days. The protease activities of GK was rapidly increased after 20 days. The total polyphenol and flavonoid contents tended to be similar and dramatically increased after 15 days. On the other hand, the DPPH radical scavenging activity of MK was higher than that of GK. These results showed that the 20-day maturation period of GK greatly enhanced its antioxidant activity and quality characteristics. It is thus expected to be used as functional materials.

재래식 간장에 표고버섯 및 천마를 첨가하여 일정기간 숙성시킨 후 분리하여 이화학적 성질 등의 변화를 측정하였다. NaCl의 함량은 대조구에 비해 실험구가 높게 나타났으며, 다른 재래식 방법으로 제조한 간장에 비해 전체적으로 염도가 낮았다. pH의 변화는 숙성이 될 수록 감소하였으며 총당과 환원당의 함량 또한 대조구에 비해 실험군이 높은 값을 보였는데 표고버섯이나 천마로부터 유래된 당의 영향으로 인해 높아진 것으로 판단된다. Protease 활성은 실험구가 6~8배 이상 높은 값을 보였고 항산화 활성에서는 천마간장이 총 폴레페놀 함량 90.23 mg/mL, 총 플라보노이드 함량 404.44 mg/mL로 가장 높은 값을 보였으며 전자 공여능 측정에서 버섯 간장이 72.71%를 나타냈다.

Keywords

References

  1. Lee BY, Yang YM, Han CK (2002) Analysis of aroma pattern of Gastrodiae rhizoma by the drying conditions, Korean J Food Sci Technol, 34, 13-17
  2. Choi JD, Im MH, Chung HC, Lee CW, Kim YH, Choi C,, Choi KS (1997) The effects of mashing and maturing conditions on the quality of Korean traditional Kanjang (soy sauce). Agri Chem Biotechnol, 40, 365-368
  3. Choi SY (1997) A study on the physiological function of traditional Kangjang and Doenjang, and development of process for functional food. Annual report of ARPC, Korea
  4. Kim JK (1997) Study on the novel antimutagenic pigments in traditional soy sauce and soybean paste. Annual report of ARPC, Korea
  5. Chun KY, Lim IS, Choi KG, Kim JJ, Kim JS, Lee SE, Kim HJ (2004) The noxiousness of the salt-dependent hypertension and the effect of the physical stimulation on the change of the hypertension-related sympathetic neurotransmitter-a study on the utilization of physical therapy facilities in senior welfare C. Korean J Gerontol Soc, 24, 1-11
  6. Society of oriental medicine (1993) Modern oriental medicine, Hakqangsa, Seoul, Korea, p 446-447
  7. Hong IP, Nam SH, Jung IY, Sung GB, Nam HW, Cheong JC, Park JS, Hur H, Lee MW (2004) Studies on the conditions of seed germination of Gastrodia elata. Korean J Mycology, 32, 39-44 https://doi.org/10.4489/KJM.2004.32.1.039
  8. Lee EJ, Kwon OJ, Im MH, Choi UK, Son DH, Lee SI, Kim DG, Cho YJ, Kim WS, Kim SH , Chung YG (2002) Chemical changes of Kangjang made with barely bean. Korean J Food Sci Technol, 34, 751-756
  9. Chung HS, Ji GE (1996) Composition and functionality of Chonma. Korean J Food Sci Technol, 28, 53-57
  10. Taguchi H, Yosioka L, Yamasaki K, Kim IH (1981) Studies on the constituents of Gastrodia elata Blume. Chem Pharm Bull, 29, 55-62 https://doi.org/10.1248/cpb.29.55
  11. Kwon JH, Byun MW, Cho HO, Kim YJ, Kim JG (1987) Effect of chemical fumigant and $\gamma$-rays on the physicochemical properties of dried oak mushrooms. Korean J Food Sci Technol, 19, 273-278
  12. Ko JW, Lee WY, Lee JH, Ha YS, Choi YH (1999) Absorption characteristics of dried shiitake mushroom powder using different drying methods. Korean J Food Sci Technol, 31, 128-197
  13. Hong JS, Kim YH(1989) Contents of free amino acids and total amino acids in Agaricus bisporus, Pleurotus ostreatus and Lentinus edodes. Korean J Food Sci Technol, 21, 58-62
  14. Cho DB, Kim DP, Choi CS (1981) Kinetic of drying shiitake mushroom, Lentinus edodes sanryun. Korean J Soc Food Nutr, 10, 53-60
  15. Choi SY, Sung NJ, Kim HJ (2006) Physicochemical characteristics of traditional Doenjang with added Lentinus edodes. Korean J Food Cookery Sci, 22, 69-79
  16. Oh JY, Kim YS, Shin DH (2002) Changes in physicochemical characteristics of low-salted kochujang with natural preservatives during fermentation. Korean J Food Sci Technol, 34, 835-841
  17. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugar and related substance. Anal Chem, 28, 350-352 https://doi.org/10.1021/ac60111a017
  18. Kim JY, Yi YH (2010) pH, acidity, color, amino acids, reducing sugars, total sugars, and alcohol in puffed millet powder containing millet takju during fermentation. Korean J Soc Food Sci, 42, 727-732
  19. Lin JY, Tang CY (2007) Determination of total phenolic and flavonoid contents in selecterd fruits and vegetables, as well as their stimulatory effects on mouse splenocyte proliferation. Food Chem, 101, 140-147 https://doi.org/10.1016/j.foodchem.2006.01.014
  20. Chang CC, Yang MH, Wen HM, Chern JC (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Food J Drug Anal, 10, 178-182
  21. Kilani S, Ammar RR, Bouhlel I, Hayder N, Mahmoud A, Ghedira K, Chekir Ghedira L (2005) Investigation of extracts from (tunisian) Cyperus rotundus as antimutagens and radical scavengers. Environ Toxicol Phar, 20, 478-484 https://doi.org/10.1016/j.etap.2005.05.012
  22. Kim HJ, Lee JJ, Cheigh MJ, Choi SY (1998) Amylase, protease, perocidase, and ascorbic acid oxidase activities of kimchi. Korean J Food Sci Technol, 30, 1333-1338
  23. Son KH, Lee HJ, Park HK, Park OJ (1998) Studies on taste compound content and reasearch on condition of comsumer attitude to traditional Korean soy sauce with varing Meju type and fermentation jar. Korean J Food Sci, 14, 464-467
  24. Chang CH (1996) Free-sugars in ordinary korean soy sauce. Agri Chem Biotech, 7, 35-37
  25. Kim YA, Kim HS, Chung MJ (1996) Physicohemical analysis of korean traditional soy sauce and commercial soy sauce. Korean J Soc Food Sci, 12, 273-279
  26. Kim JS, Moon GS, Lee YS (2006) Chomaticity and brown pigment patterns of soy sauce and Uhyukjang, Korean traditional fermented soy sauce. Korean J Food Cookery Sci, 23, 642-649
  27. Shin JH, Kang MJ, Yang SM, Lee SJ, Ryu JH, Kim RJ, Sung NJ (2010) Comparison of physicochemical properties and antioxidant activities of Korean traditional Kangjang and garlic added Kangjang. J Agric Life Sci, 44, 39-48
  28. Jang DK, Woo KL, Lee SC (2003) Quality characteristics of soy sauces containing shiitake mushroom. Korean J Soc Agric Chem Biotechnol, 46, 220-224
  29. Chae SH (2000) Color characteristics and antioxidizing ability of Korean traditional soy sauce prepared from different conditions. Ph D Thesis, Yonsei University, Seoul, Korea
  30. Kwon SH, Choi JH, Ko YR, Shon MY, Park SK (2003) Changes in free sugars, organic acid and fatty acid composition of Kanjang prepared with different cooking conditions of whole black bean. Korean J Food Preserv, 10, 333-338
  31. Choi SR, Jang I, Kim CS, You DH, Kim JY, Kim YG, Ahn YS, Kim JM, Kim YS, Kyoung WS (2011) Changes of components and quality in Gastrodiae rhizoma by different dry methods. Korean J Medicinal Crop Sci, 19, 354-361 https://doi.org/10.7783/KJMCS.2011.19.5.354
  32. Jeon MS, Sohn KH, Chae SH, Park HK, Jeon HJ (2002) Color characteristics of Korean traditional soy sauces prepared under different processing conditions. Korean J Soc Food Sci Nutr, 31, 32-38 https://doi.org/10.3746/jkfn.2002.31.1.032
  33. Kim BS (2007) Changes of enzyme activity and physiological functionality of traditional Doenjang during fermetation in the using bacillus sp. SP-KSW3. Korean J Food Preserv, 14, 545-551
  34. Park MR, Yoo C, Chang YN, Ahn BY (2012) Change of total polyphenol content of fermented Gastrodia elata Blume and Radical Scavenging. Korean J Plant Res, 25, 379-386 https://doi.org/10.7732/kjpr.2012.25.4.379
  35. Heo JC, Park JY, An SM, Lee JM, Yun CY, Shin HM, Kwon TK, Lee SH (2006) Anti oxidant and anti tumor activities of crude extracts by Gastrodia elata Blume. Korean J Food Preserv, 13, 83-87
  36. Liu J, Mori A (1992) Antioxidant and free radical scavenging activities of Gastrodia elata Bl. and Uncaria rhynchophylla (Miq.) Jacks. neuropharmalcol, 31, 1287-1298 https://doi.org/10.1016/0028-3908(92)90058-W
  37. Li N, Wang KI, Chen JJ, Zhou J (2007) Phenolic compounds from the rhizomes of Gastrodia elata. Asian J Natural Products Res, 9, 373-377 https://doi.org/10.1080/10286020600780979
  38. Lin JH, Liu YC, Hau JP, Wen KC (1996) Parishins b and c from rhizomes of Gastrodia elata. Phytochem, 42, 549-551 https://doi.org/10.1016/0031-9422(95)00955-8
  39. Taguchi H, Yosioka I, Yamasaki K, Kim IL (1996) Strudies on the constituents of G. elata Blume. Chem Pharm Bull, 29, 55-62

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