대한환경공학회지 (Journal of Korean Society of Environmental Engineers)

  • 제36권5호
  • /
  • Pages.352-358
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  • 2014
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  • 1225-5025(pISSN)
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  • 2383-7810(eISSN)
대한환경공학회 (Korean Society of Environmental Engineers)
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신균주 Rhodococcus sp. YSPW01과 YSPW02를 이용한 수중 내 고농도 Perchlorate 환원 특성 연구

Reduction of Highly Concentrated Perchlorate in Aqueous Medium by Newly Isolated Bacterial Strains Rhodococcus sp. YSPW01 and YSPW02

  • 이상훈 (연세대학교 환경공학과) ;
  • 황재훈 (연세대학교 환경공학과) ;
  • ;
  • 이대성 (동아대학교 에너지.자원공학과) ;
  • 전병훈 (연세대학교 환경공학과)
  • Lee, Sang-Hoon (Department of Environmental Engineering, Yonsei University) ;
  • Hwang, Jae-Hoon (Department of Environmental Engineering, Yonsei University) ;
  • Akhil, Kabra (Department of Environmental Engineering, Yonsei University) ;
  • Lee, Dae Sung (Department of Energy & Mineral Resources Engineering, Dong-A University) ;
  • Jeon, Byong-Hun (Department of Environmental Engineering, Yonsei University)
  • 투고 : 2013.07.26
  • 심사 : 2014.05.09
  • 발행 : 2014.05.31
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초록

수중 내 함유된 고농도 perchlorate의 환원가능성을 perchlorate 환원 미생물인 신균주 Rhodococcus sp. YSPW01과 YSPW02로 검토하였다. Perchlorate 환원 미생물은 혐기소화조 슬러지에서 분리 배양하였으며, perchlorate 환원능을 검토하기 위해 전자공여체인 acetate를 사용하였다. YSPW01과 YSPW02는 perchlorate $82mg\;L^{-1}$와 acetate $550mg\;L^{-1}$에서 회분식 실험을 수행하였을 때, 반응 26시간과 9시간 후 각각 정상상태에 도달하였다. 이 때 perchlorate 환원은 8, 7시간 이내에 초기농도 $82mg\;L^{-1}$에서 검출한계 이하($10{\mu}g\;L^{-1}$)까지 제거되었다. 반응조 내에 acetate:perchlorate (w:w)비를 1:1에서 5:1로 증가한 결과, perchlorate의 제거 속도는 YSPW01과 YSPW02 모두 2.1, $3.2mg\;L^{-1}h^{-1}$에서 15, $15.5mg\;L^{-1}h^{-1}$로 약 4.5배 증가하였고, 최종 perchlorate는 검출한계 이하까지 제거되었다. 본 연구결과, Rhodococcus sp. YSPW01 및 YSPW02는 고농도 perchlorate 제거에 적합하며, 신균주를 고농도 perchlorate 제거를 위한 생물학적 처리공정에 응용가능 할 것으로 판단된다.

The feasibility of perchlorate reducing-bacteria isolated from the sludge of an anaerobic digester was determined using ammonium perchlorate in aqueous medium. Growth kinetics of the two perchlorate reducing bacteria including Rhodococcus sp. YSPW01 and YSPW02 were investigated using acetate as the electron donor in batch experiment. The growth of YSPW01 and YSPW02 reached a steady-state at 26 and 9 h, respectively. The initial perchlorate concentration was completely reduced within 8 and 7 h by YSPW01 and YSPW02, respectively. The reduction rates were 2.1 and $15mg\;L^{-1}h^{-1}$ for YSPW01, and 3.2 and $15.5mg\;L^{-1}h^{-1}$ for YSPW02, at 1:1 and 5:1 ratios of acetate:perchlorate (w:w), respectively. In this study, the bacteria Rhodococcus sp. YSPW01 and YSPW02 demonstrated a potential for the perchlorate reduction, which could be further investigated for development of an efficient strategy to treat the perchlorate contaminated waters.

키워드

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