Biomolecules & Therapeutics

  • 제22권3호
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  • Pages.246-253
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  • 2014
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  • 1976-9148(pISSN)
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  • 2005-4483(eISSN)
한국응용약물학회 (The Korean Society of Applied Pharmacology)
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Neuroprotective Effect of Steamed and Fermented Codonopsis lanceolata

  • Weon, Jin Bae (Department of Medical Biomaterials Engineering, College of Biomedical Science) ;
  • Yun, Bo-Ra (Department of Medical Biomaterials Engineering, College of Biomedical Science) ;
  • Lee, Jiwoo (Department of Medical Biomaterials Engineering, College of Biomedical Science) ;
  • Eom, Min Rye (Department of Medical Biomaterials Engineering, College of Biomedical Science) ;
  • Ko, Hyun-Jeong (Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University) ;
  • Lee, Hyeon Yong (Department of Teaics, Seowon University) ;
  • Park, Dong-Sik (Department of Agrofood Resources, Functional food & Nutrition Division) ;
  • Chung, Hee-Chul (Newtree CO., LTD.) ;
  • Chung, Jae Youn (Newtree CO., LTD.) ;
  • Ma, Choong Je (Department of Medical Biomaterials Engineering, College of Biomedical Science)
  • 투고 : 2014.02.17
  • 심사 : 2014.05.08
  • 발행 : 2014.05.31
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초록

Codonopsis lanceolata has been used as an herbal medicine for several lung inflammatory diseases, such as asthma, tonsillitis, and pharyngitis. Previously, we showed the neuroprotective effect of steamed and fermented C. lanceolata (SFC) in vitro and in vivo. In the current study, the treatment of HT22 cells with SFC decreased glutamate-induced cell death, suggesting that SFC protected HT22 cells from glutamate-induced cytotoxicity. Based on these, we sought to elucidate the mechanisms of the neuroprotective effect of SFC by measuring the oxidative stress parameters and the expression of Bax and caspase-3 in HT22 cells. SFC reduced contents of ROS, $Ca^{2+}$ and NO. Moreover, SFC restored contents of glutathione and glutathione reductase as well as inhibited Bax and caspase-3 activity in HT22 cells. These results indicate that steamed and fermented C. lanceolata (SFC) extract protected HT22 cells by anti-oxidative effect and inhibition of the expression of Bax and caspase-3.

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