Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Involvement of Transglutaminase-2 in α-MSH-Induced Melanogenesis in SK-MEL-2 Human Melanoma Cells

  • Kim, Hyun Ji (BK21PLUS R-FIND team, College of Pharmacy, Dongguk University) ;
  • Lee, Hye Ja (BK21PLUS R-FIND team, College of Pharmacy, Dongguk University) ;
  • Park, Mi Kyung (BK21PLUS R-FIND team, College of Pharmacy, Dongguk University) ;
  • Gang, Kyung Jin (BK21PLUS R-FIND team, College of Pharmacy, Dongguk University) ;
  • Byun, Hyun Jung (BK21PLUS R-FIND team, College of Pharmacy, Dongguk University) ;
  • Park, Jeong Ho (BK21PLUS R-FIND team, College of Pharmacy, Dongguk University) ;
  • Kim, Mi Kyung (BK21PLUS R-FIND team, College of Pharmacy, Dongguk University) ;
  • Kim, Soo Youl (National Cancer Center) ;
  • Lee, Chang Hoon (BK21PLUS R-FIND team, College of Pharmacy, Dongguk University)
  • Received : 2014.03.11
  • Accepted : 2014.05.07
  • Published : 2014.05.31
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Abstract

Skin hyperpigmentation is one of the most common skin disorders caused by abnormal melanogenesis. The mechanism and key factors at play are not fully understood. Previous reports have indicated that cystamine (CTM) inhibits melanin synthesis, though its molecular mechanism in melanogenesis remains unclear. In the present study, we investigated the effect of CTM on melanin production using ELISA reader and the expression of proteins involved in melanogenesis by Western blotting, and examined the involvement of transglutaminase-2 (Tgase-2) in SK-MEL-2 human melanoma cells by gene silencing. In the results, CTM dose-dependently suppressed melanin production and dendrite extension in a-MSH-induced melanogenesis of SK-MEL-2 human melanoma cells. CTM also suppressed a-MSH-induced chemotactic migration as well as the expressions of melanogenesis factors TRP-1, TRP-2 and MITF in a-MSH-treated SK-MEL-2 cells. Meanwhile, gene silencing of Tgase-2 suppressed dendrite extension and the expressions of TRP-1 and TRP-2 in a-MSH-treated SK-MEL-2 cells. Overall, these findings suggested that CTM suppresses a-MSH-induced melanogenesis via Tgase-2 inhibition and that therefore, Tgase-2 might be a new target in hyperpigmentation disorder therapy.

Keywords

References

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