Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Britanin Suppresses IgE/Ag-Induced Mast Cell Activation by Inhibiting the Syk Pathway

  • Lu, Yue (School of Pharmacy, Shanghai University of Traditional Chinese Medicine) ;
  • Li, Xian (College of Pharmacy, Yeungnam University) ;
  • Park, Young Na (College of Pharmacy, Yeungnam University) ;
  • Kwon, Okyun (College of Pharmacy, Yeungnam University) ;
  • Piao, Donggen (College of Pharmacy, Yeungnam University) ;
  • Chang, Young-Chae (Department of Pathology, Catholic University of Daegu School of Medicine) ;
  • Kim, Cheorl-Ho (Department of Biological Science, Sungkyunkwan University) ;
  • Lee, Eunkyung (Research and Development Division, Korean Promotion Institute for Traditional Medicine Industry) ;
  • Son, Jong Keun (College of Pharmacy, Yeungnam University) ;
  • Chang, Hyeun Wook (College of Pharmacy, Yeungnam University)
  • Received : 2014.03.25
  • Accepted : 2014.05.07
  • Published : 2014.05.31
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Abstract

The aim of this study was to determine whether britanin, isolated from the flowers of Inula japonica (Inulae Flos), modulates the generation of allergic inflammatory mediators in activated mast cells. To understand the biological activity of britanin, the authors investigated its effects on the generation of prostaglandin $D_2$ ($PGD_2$), leukotriene $C_4$ ($LTC_4$), and degranulation in IgE/Ag-induced bone marrow-derived mast cells (BMMCs). Britanin dose dependently inhibited degranulation and the generations of $PGD_2$ and $LTC_4$ in BMMCs. Biochemical analyses of IgE/Ag-mediated signaling pathways demonstrated that britanin suppressed the phosphorylation of Syk kinase and multiple downstream signaling processes, including phospholipase $C{\gamma}1$ ($PLC{\gamma}1$)-mediated calcium influx, the activation of mitogen-activated protein kinases (MAPKs; extracellular signal-regulated kinase 1/2, c-Jun $NH_2$-terminal kinase and p38), and the nuclear factor-${\kappa}B$ ($NF-{\kappa}B$) pathway. Taken together, the findings of this study suggest britanin suppresses degranulation and eicosanoid generation by inhibiting the Syk-dependent pathway and britanin might be useful for the treatment of allergic inflammatory diseases.

Keywords

References

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