한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제43권5호
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  • Pages.741-748
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  • 2014
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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반응표면분석법을 이용한 인진쑥 유효성분의 에탄올 추출조건 최적화

Optimization of Ethanol Extraction Conditions for Artemisis capillaris Effective Components Using Response Surface Methodology

  • Kim, Seong-Ho (Dept. of Food Science and Technology, Daegu University)
  • 투고 : 2014.03.07
  • 심사 : 2014.04.15
  • 발행 : 2014.05.31
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초록

본 연구는 인진쑥의 광범위한 약리작용과 다양한 생리활성을 이용한 식품소재로서의 개발을 위하여 반응표면분석법에 의한 인진쑥 유효성분의 에탄올 추출특성을 모니터링 하여 최적의 추출조건을 예측하였다. 에탄올을 이용한 추출공정에서 중요변수로 추출온도($X_1$; 60, 70, 80, 90 및 $100^{\circ}C$), 추출시간($X_2$; 1, 2, 3, 4 및 5 hr) 및 에탄올 농도($X_3$; 0, 20, 40, 60 및 80%)를 각각 독립변수로 설정하였고 추출물의 품질특성 즉 가용성 고형분 함량($Y_1$), chlorogenic acid($Y_2$) 및 coumaric acid($Y_3$) 등을 종속변수로 하여 반응표면 회귀분석을 실시하였다. 고형분 함량에 대한 예측된 최적조건은 추출온도 $87.65^{\circ}C$, 추출시간 3.19시간, 에탄올 농도 42.40%이었고 chlorogenic acid는 추출온도 $84.30^{\circ}C$, 추출시간 3.14시간, 에탄올 농도 47.85%이었으며 coumaric acid는 추출온도 $83.45^{\circ}C$, 추출시간 3.40시간 및 에탄올 농도 45.39%로 나타났다. 각 변수에 대한 회귀식을 도출하여 인진쑥 추출물의 각 유효성분들에 대한 최적 추출조건을 superimposing 한 결과, 추출온도 $85{\sim}90^{\circ}C$, 추출시간 3~4시간 및 에탄올 농도 40~50%로 예측되었다. 이때 각 성분의 함량은 가용성 고형분 함량 1.09%, chlorogenic acid 25.66 mg%, coumaric acid 20.25 mg%이었다.

This study was conducted to monitor the quality characteristics of Artemisis capillaris ethanolic extract by response surface methodology. The independent variables were extraction temperature ($X_1$; 60, 70, 80, 90, and $100^{\circ}C$), extraction time ($X_2$; 1, 2, 3, 4, and 5 hr), and ethanol concentration ($X_3$; 0, 20, 40, 60, and 80%). Soluble solid content ($Y_1$), chlorogenic acid content ($Y_2$), and coumaric acid content ($Y_3$), etc. were analyzed as the dependent variables. Estimated optimal conditions for soluble solids were an extraction temperature of $87.65^{\circ}C$, extraction time of 3.19 hr, and ethanol concentration of 42.40%. The optimal extraction conditions for chlorogenic acid were $84.30^{\circ}C$, 3.14 hr, and 47.85%, respectively. Further, those for coumaric acid were $83.45^{\circ}C$, 3.40 hr, and 45.39%, respectively. Extraction conditions for effective components of Artemisis capillaris were superimposed by response surface plots on optimization extraction condition of each dependent variable, including soluble solid, chlorogenic acid, and coumaric acid contents. As a result, superimposed extraction conditions were $80{\sim}90^{\circ}C$, 3~4 hr, and 40~50%, respectively. Under these conditions, soluble solid, chlorogenic acid, and coumaric acid contents were 1.09%, 25.66 mg%, and 20.25 mg%, respectively.

키워드

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피인용 문헌

  1. 전처리 방법을 달리한 섬애약쑥 에탄올 추출물의 이화학적 특성 vol.27, pp.1, 2014, https://doi.org/10.5352/jls.2017.27.1.23