Journal of Korean Society of Environmental Engineers (대한환경공학회지)

Korean Society of Environmental Engineers (대한환경공학회)
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Toxic Interactions of Perfluorinated Compounds (PFCs) with Heavy Metals Using Vibrio fischeri

발광박테리아 Vibrio fischeri를 이용한 과불화합물과 중금속의 복합독성평가

  • Lee, Woo-Mi (Geum River Basin Environmental Office) ;
  • Kim, Ji-Sung (Department of Construction Environment Engineering, University of Science and Technology) ;
  • Kim, Il-Ho (Department of Construction Environment Engineering, University of Science and Technology) ;
  • Kim, Seog-Ku (Department of Construction Environment Engineering, University of Science and Technology) ;
  • Yoon, Young-Han (Environmental Engineering Research Division, Korea Institute of Construction Technology)
  • 이우미 (금강유역환경청) ;
  • 김지성 (과학기술연합대학원대학교 건설환경공학과) ;
  • 김일호 (과학기술연합대학원대학교 건설환경공학과) ;
  • 김석구 (과학기술연합대학원대학교 건설환경공학과) ;
  • 윤영한 (한국건설기술연구원 환경연구실)
  • Received : 2013.10.25
  • Accepted : 2014.02.03
  • Published : 2014.02.28
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Abstract

The object of this study was to evaluate the combined toxic interactions of the perfluorooctanoic acid (PFOA) or perfluorooctane sulfonate (PFOS) with six heavy metals (Cu, Zn, Cr, Cd, Hg, and Pb). The individual and combined toxic effects were assessed using the Vibrio fischeri assay. In case of the individual toxicity, PFOA was higher toxic than PFOS and toxicity of PFOA and PFOS were lower than heavy metal. In the toxicity of heavy metals, the $Hg^{2+}$ was found to be most toxic followed by $Pb^{2+}$, $Cr^{6+}$, $Cu^{2+}$, $Zn^{2+}$, and $Cd^{2+}$. The combined toxicity of PFOA or PFOS with $Cr^{6+}$ were synergistic effect because the $EC_{50}$ mix values were less than 1 TU. PFOA + $Zn^{2+}$, PFOS + $Zn^{2+}$, PFOA + $Cd^{2+}$ and PFOS + $Cd^{2+}$ produced addictive effect. Except in these case, all of binary mixtures show antagonistic effect. This study proved potential risk of coexistent with perfluorinated compounds and heavy metals in water environment.

본 연구에서는 과불화합물(PFOA, PFOS)과 수계에 보편적으로 존재하는 중금속(Cu, Zn, Cr, Cd, Pb, Hg)의 복합독성을 Vibrio fischeri를 이용하여 평가하였다. PFOA와 PFOS의 경우, 30 min-$EC_{50}$값이 각각 134.21 (119.54-150.68)와 235.97 (180.96-307.70) mg/L로 PFOS 보다는 PFOA의 독성이 높은 것으로 나타났다. 한편 중금속류의 독성은 $Hg^{2+}$의 독성이 가장 높았으며, 이어서 $Pb^{2+}$, $Cr^{6+}$, $Cu^{2+}$, $Zn^{2+}$, $Cd^{2+}$ 순으로 높은 독성민감도를 나타냈다. 과불화합물류와 중금속류의 복합독성의 경우, PFOA와 PFOS 모두 $Cr^{6+}$과 공존할 시 상승효과가 나타났으며, PFOA + $Zn^{2+}$, PFOS + $Zn^2$, PFOA + $Cd^{2+}$, PFOS + $Cd^{2+}$의 조합은 상가 효과를 나타냈다. 이외의 복합물질은 모두 길항작용을 하는 것으로 확인되었다. 본 연구에서는 과불화합물인 PFOA, PFOS와 중금속 복합물질들의 조합에 따른 상호작용이 상이함을 확인하였고 이 결과로부터 과불화합물과 중금속 공존할 때 수환경에 야기할 수 있는 잠재적 위해성을 예측할 수 있다.

Keywords

References

  1. Parsons, J. R., Saez, M., Dolfing, J. and de Voogt, P., "Biodegradation of perfluorinated compounds," Rev. Environ. Contam. Toxicol., 196, 53-71(2008).
  2. Liu, W., Chen, S., Quan, X. and Jin, Y., "Toxic effect of serial perfluorosulfonic and perfluorocarboxylic acid on the membrane system of a freshwater alga measured by flow cytometry," Environ. Toxicol. Chem., 27(7), 1597-1604(2008). https://doi.org/10.1897/07-459.1
  3. Suja, F., Pramanik, B. K. and Zain, S. M., "Contamination, bioaccumulation and toxic effects of perfluorinated chemicals (PFCs) in the water environment: a review paper," Water Sci. Technol., 60(6), 1533-1544(2009). https://doi.org/10.2166/wst.2009.504
  4. Agency for Toxic Substance and Disease Registry, "Draft toxicological profile for perfluoroalkyls," http://www.atsdr.cdc.gov/toxprofiles/tp200.pdf(2009).
  5. United Nations Environment Program, "Risk profile on perfluorooctane sulfonate," Stockholm Convention on Persistent Organic Pollutants Review Committee, Geneva(2006).
  6. Benford, D., de Boer, J., Carere, A., di Domenico, A., Johansson, N., Schrenk, D., Schoeters, G., de Voogt, P. and Dellatte, E., "Opinion of the Scientific Panel on Contaminants in the Food chain on perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA) and their salts," EFSA, 653, 1-131(2008).
  7. Moermond, C. T. A., Verbruggen, E. M. J. and Smit, C. E., "Environmental risk limits for PFOS: A proposal for water quality standards in accordance with the Water Framework Directive," RIVM Report 601714013(2010).
  8. Jensen, A. A. and Leffers, H., "Emerging endocrine disrupters: perfluoroalkylated substances," Int. J. Androl., 31, 161-169(2008). https://doi.org/10.1111/j.1365-2605.2008.00870.x
  9. Stockholm Convention, "Acceptable purposes: PFOS and PFOSF," http://chm.pops.int/Implementation/Exemptions/AcceptablePurposesPFOSandPFOSF/tabid/794/Default.aspx#_ftnref1(2013).
  10. Ministry of Environment/National Institute of Environmental Research, "Press release; research of endocrine disrupters in industrial area,"(2009).
  11. Marugan, J., Bru, D., Pablos, C. and Catala, M., "Comparative evaluation of acute toxicity by Vibrio fischeri and fern spore based bioassays in the follow-up of toxic chemicals degradation by photocatalysis," J. Hazard. Mater., 213-214, 117-122(2012). https://doi.org/10.1016/j.jhazmat.2012.01.075
  12. Power, E. A. and Boumphrey, R., "International trends in bioassay use for effluent management," Ecotoxicol., 13, 377-398(2004). https://doi.org/10.1023/B:ECTX.0000035290.89590.03
  13. Rodea-Palomares, I., Leganes, F., Rosal, R. and Fernandez-Pinas, F., "Toxicological interactions of perfluorooctane sulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) with selected pollutants," J. Hazard. Mater., 201-202, 209-218(2012). https://doi.org/10.1016/j.jhazmat.2011.11.061
  14. Keiter, S., Baumann, L., Farber, H., Holbech, H., Skutlarek, D., Engwall, M. and Braunbeck, T., "Long-term effects of a binary mixture of perfluorooctane sulfonate (PFOS) and bisphenol A (BPA) in zebrafish (Danio reiro)," Aquat. Toxicol., 118-119, 116-129(2012). https://doi.org/10.1016/j.aquatox.2012.04.003
  15. Kim, S., Ji, K., Lee, S., Lee, J., Kim, J., Kim, S., Kho, Y. and Choi, K., "Perfluorooctane sulfonic acid exposure increases cadmium toxicity in early life stage of zebrafish, Danio rerio," Environ. Toxicol. Chem., 30, 870-877(2010).
  16. Liu, W., Zhang, Y. B., Quan, X., Jin, Y. H. and Chen, S., "Effect of perfluorooctane sulfonate on toxicity and cell uptake of other compounds with different hydrophobicity in green alga," Chemosphere, 75, 405-409(2009). https://doi.org/10.1016/j.chemosphere.2008.11.084
  17. KSIISO, "Water quality - determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (luminescent bacteria test) - part3 : method using freeze-dried bacteria," KSIISO 11348:3(2009).
  18. Hamilton, M. A., Russo, R. C. and Thurston, R. V., "Trimmed Spearman-Karber method for estimating median lethal concentrations in toxicity bioassays," Environ. Sci. Technol., 11, 714-719(1977); Correction 12, 417(1978). https://doi.org/10.1021/es60130a004
  19. Dunnett, C. W., "Multiple comparison proceure for comparing several treatments with a control," J. Am. Stat. Assoc., 50, 1096-1121(1995).
  20. Van der Geest, H. G., Greve, G. D., Blivin, M. E., Kraak, M. H. S. and van Gestel, C. A. M., "Mixture toxicity of copper and diazinon to larvae of the mayfly judging additivity at different effect levels," Environ. Toxicol. Chem., 19(12), 2900-2905(2000). https://doi.org/10.1002/etc.5620191208
  21. An, Y. J. and Lee, W. M., "Decreased toxicity to terrestrial plants associated with a mixture of methyl-tert butyl ether and its metabolite tert-butyl alcohol," Environ. Toxicol. Chem., 26(8), 1711-1716(2007). https://doi.org/10.1897/06-434R1.1
  22. Nobels, I., Dardenne, F., de Coen, W. and Blust, R., "Application of a multiple endpoint bacterial reporter assay to evaluate toxicological relevant endpoints of perfluorinated compounds with different functional groups and varying chain length," Toxicol. in vitro, 24, 1768-1774(2010). https://doi.org/10.1016/j.tiv.2010.07.002
  23. Jennings, V. L. K., Rayner-brandes, M. H. and Bird, D. J., "Assessing chemical toxicity with the bioluminescent photobacterium (Vibrio fischeri): A comparison of three comercial systems," Water Res., 35, 3448-3456(2001). https://doi.org/10.1016/S0043-1354(01)00067-7
  24. Rosal, R., Rodea-Palomares, I., Boltes, K., Fernandez-Pinas, F., Leganes, F. and Petre, A., "Ecotoxicological assessment of surfactants in the aquatic environment: Combined toxicity of docusate sodium with chlorinated pollutants," Chemosphere, 81, 288-293(2010) https://doi.org/10.1016/j.chemosphere.2010.05.050
  25. Lee, C. W., Kim, H. M. and Choi, K. H., "Toxicity assessment of PFOA and PFOS using freshwater flea Hyalella azteca," J. Environ. Toxicol., 22(3), 271-277(2007)
  26. Kim, K. Cho, J., Yoon, J., Lee, C., Choi, K., Kim, H. and Ryu, J., "Toxicity evaluation of perfluorinated compounds using Daphnia magna," Environ. Health Toxicol., 25, 153-159(2010).
  27. Gueguena, C., Gilbina, R., Pardosa, M. and Dominika, J., "Water toxicity and metal contamination assessment of a polluted river: the Upper Vistula River (poland)," Appl. Geochem., 19, 153-162(2004). https://doi.org/10.1016/S0883-2927(03)00110-0
  28. Cho, J. C., Park, K. J., Ihm, H. S., Park J. E., Kim, S. Y., Kang, I., Lee, K. H., Jahng, D., Lee, D. H. and Kim, S. J., "A novel continuous toxicity test system using a luminously modified freshwater bacterium," Biosens. Bioelectron., 20, 338-344(2004). https://doi.org/10.1016/j.bios.2004.02.001
  29. Son, Y. H, Lee, H. M., Ryu, J. K. and Rhu, H. I., "A study on biotoxicity assessment of toxic materials on Vibrio fischeri," Kor. J. Environ. Toxicol., 9(2), 41-50(1994).
  30. Tsiridis, V., Petala, M., Samaras, P., Hadjispyrou, S., Sakellaropoulos, G. and Kungolos, A., "Interactive toxic effects of heavy metals and humic acids on Vibrio fischeri," Ecotoxicol. Environ. Saf., 63, 158-167(2006). https://doi.org/10.1016/j.ecoenv.2005.04.005
  31. Lopez-Roldan, R., Kazlauskaite, L., Ribo, J., Carme Riva, M., Gonzalez, S., Cortina, J. L., "Evaluation of an automated luminescent bacteria assay for in situ aquatic toxicity determination," Sci. Total. Environ., 440, 307-313(2012). https://doi.org/10.1016/j.scitotenv.2012.05.043
  32. Greene, J. C., Miller, W. E., Debacon, M. K., Long, M. A. and Bartels, C. L., "A comparison of three microbial assay procedures for measuring toxicity of chemicals residues," Arch. Environ. Contam. Toxicol., 14, 659-667(1985). https://doi.org/10.1007/BF01055772