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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Effects of Ukgansan (Yokukansan in Japanese, Yigansan in chinese) on the Locomotor Velocity and Glutamate-Induce Paroxysm in Planarian

Planarian 모델을 이용한 억간산의 항발작 효과

  • Park, Woong (Department of Pharmaceutical Engineering, Woosuk University) ;
  • Yoo, Du Man (Department of Pharmaceutical Engineering, Woosuk University) ;
  • So, June No (Department of Pharmaceutical Engineering, Woosuk University)
  • 박웅 (우석대학교 제약공학과) ;
  • 유두만 (우석대학교 제약공학과) ;
  • 소준노 (우석대학교 제약공학과)
  • Received : 2014.02.07
  • Accepted : 2014.02.24
  • Published : 2014.02.27
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Abstract

Planaria were recently reported to be a simple and sensitive model to investigate the mechanistic aspect of seizure and to screen potential anticonvulsants. Using planarian model, we analyzed the pharmacological effect of ukgansan (UGS), an oriental herbal medicine containing seven medicinal herbs, on the planarian locomotor velocity (pLMV) and glutamate-induced seizure-like activity (pSLA). To test whether D. japonica is suitable for studying anti-seizure agents, we investigated the effect of glutamate on pLMV and pSLA in D. japonica. In the present study we first confirmed that pSLA in D. japonica was induced by L-glutamate. Glutamate significantly produced pSLA in a dose dependent manner, but did not affect pLMV. These glutamate-induced paroxysms were decreased by antiepileptic drug, topiramate. A similar inhibitory effect on glutamate-induced pSLA was observed after the treatment of UGS. The present results suggest that UGS and its active constituents possess useful substance inhibiting seizure in planarian and that D. japonica provides a convenient model to search active herbs containing anti-seizure activity.

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References

  1. Loscher, W., H. Klitgaard, R. E. Twyman, and D. Scmidt (2013) New avenues for anti-epileptic drug discovery and development. Nature Rev. Drug Discov. 12: 757-776. https://doi.org/10.1038/nrd4126
  2. Lason, W., M. Chlebicka, and K. Rejdak (2013) Research advances in basic mechanisms of seizures and antiepileptic drug action. Pharmacol. Rep. 65: 787-801. https://doi.org/10.1016/S1734-1140(13)71060-0
  3. Baraban, S. C. (2007) Emerging epilepsy models: Insight from mice, flies, worms and fish. Curr. Oin. Neurol. 20: 164-168. https://doi.org/10.1097/WCO.0b013e328042bae0
  4. Rink, J. C. (2013) Stem cell systems and regeneration in planaria. Dev. Genes Evol. 223: 67-84. https://doi.org/10.1007/s00427-012-0426-4
  5. Buttarelli, F. R., C. Pellicano, and F. E. Pontieri (2008) Neuropharmacology and behavior in planarians: Translation to mammals. Comp. Biochem. Physiol. Part C 147: 399-408.
  6. Rawls, S. M., T. Thomas, M. Adeola, T. Patil, N. Raymondi, A. Poles, M. Loo, and R. B. Raffa (2009) Topiramate antagonizes NMDA- and AMPA-induced seizure-like activity in planarians. Pharmacol. Biochem. Behav. 93: 363-367. https://doi.org/10.1016/j.pbb.2009.05.005
  7. Raffa, R. B., K. E. Finno, C. S. Tallarida, and S. M. Rawls (2010) Topiramate-antagonism of L-glutamate-induced paroxysms in planarians. Eur. J. Pharmacol. 643: 150-153.
  8. Hyun, W. H. and S. R. Lee (1994) Studies on the effect of anticonvulsion and analgesic by ukgansan and ukgansangami. J. Oriental Neuropsychiatry 5: 69-79.
  9. Kamei, J., S. Miyata, and M. Ohsawa (2009) Involvement of benzodiazepine system in the anxiolytic-effect of Yokukansan (Yi-gan san). Progress in Neuro-Psychopharmacol. Biol. Psychiatry 33: 1431-1437. https://doi.org/10.1016/j.pnpbp.2009.07.023
  10. Tabuchi, M., T. Yamaguchi, S. Iizuka, S. Imamura, Y. Ikarashi, and Y. Kase (2009) Ameliorative effects of Yukukansan, a traditional Japanese medicine, on learning and non-cognitive disturbances in the Tg2576 mouse model of Alzheimer's disease. J. Ethnopharmacol. 122: 157-162. https://doi.org/10.1016/j.jep.2008.12.010
  11. Kawakami, Z., H. Kanno, T. Ueki, K. Terawaki, M. Tabuchi, Y. Ikarashi, and Y. Kase (2009) Neuroprotective effects of Yokukansan, a traditional Japanese medicine, on glutamate-mediated excitotoxicity in cultured cells. Neuroscience 159: 1397-1407. https://doi.org/10.1016/j.neuroscience.2009.02.004
  12. Vincent, P. and C. Muller (2009) Kainate receptors in epilepsy and excitotoxicity. Neuroscience 158: 309-323. https://doi.org/10.1016/j.neuroscience.2008.02.066
  13. Raffa, R. B., L. J. Holland, and R. J. Schulingkamp (2001) Quantitative assessment of dopamine D2 antagonist activity using invertebrate (Planaria) locomotion as a functional endpoint. J. Pharmacol. Toxicol. Methods. 45: 223-226. https://doi.org/10.1016/S1056-8719(01)00152-6
  14. Buttarelli, F. R., C. Pellicano, and F. E. Pontieri (2008) Neuropharmacology and behavior in planarians: Translations to mammals. Comparative Biochem. Physiol. Part C 147: 399-408.
  15. Buttarelli, F. R., F. E. Pontieri, V. Margotta, and G. Palladini (2000) Acetylcholine/dopamine interactions in planaria. Comparative Biochem. Physiol. Part C 125: 225-231.
  16. Passarelli, F., A. Merante, F. E. Pontieri, V. Margotta, G. Venturini, and G. Palladini (1999) Opioid-dopamine interaction in planaria: a behavioral study. Comparative Biochem. Physiol. Part C 124: 51-55. https://doi.org/10.1016/S0305-0491(99)00096-6
  17. Rawls, S. M., T. Gomez, and R. B. Raffa (2007) An NMDA antagonist (LY 235959) attenuates abstinence-induced withdrawal of planarians following acute exposure to a cannabinoid agonist (WIN 52212-2). Pharmacol. Biochem. Behav. 86: 499-504. https://doi.org/10.1016/j.pbb.2007.01.010
  18. Rowley, N. M., K. K. Madsen, A. Schousboe, and H. S. White (2012) Glutamate and GABA synthesis, release, transport and metabolism as targets for seizure control. Neurochem. Int. 61: 546-558. https://doi.org/10.1016/j.neuint.2012.02.013
  19. Ramakrishnan, L. and C. DeSaer (2011) Cabamazepine inhibits distinct chemoconvulsant-induced sezure-like activity in Dugesia tigrina. Pharmacol. Biochem. Behav. 99: 665-670. https://doi.org/10.1016/j.pbb.2011.06.003
  20. Kawakami, Z., H. Kanno, Y. Ikarashi, and Y. Kase (2011) Yokukansan, a kampo medicine, protects against glutamate cytotoxicity due to oxidative stress in PC12 cells. J. Ethnopharmacol. 134: 74-81. https://doi.org/10.1016/j.jep.2010.11.063
  21. Kim, K. S., G. K. Sung, and B. S. Moon (1998) An experimental study on the anti-epileptic effects of ukgansan. Kor. Intern. Med. Soc. 19: 57-72.