KSBB Journal

  • 제29권2호
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  • Pages.81-86
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  • 2014
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  • 1225-7117(pISSN)
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  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
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Lactobacillus crispatus KLB46의 스트레스 전처리시 열 내성 증진효과

Improved Cell Viability of Lactobacillus crispatus KLB46 by Stress Adaptation

  • Kwak, Dae-Yung (Department of Biological Engineering, College of Engineering, Inha University) ;
  • Kang, Chang-Ho (Department of Biological Engineering, College of Engineering, Inha University) ;
  • Jeon, HanEul (Department of Biological Engineering, College of Engineering, Inha University) ;
  • So, Jae-Seong (Department of Biological Engineering, College of Engineering, Inha University)
  • 투고 : 2014.02.10
  • 심사 : 2014.03.21
  • 발행 : 2014.04.30
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초록

Lactobacilli, the dominant species of microorganisms in the vaginal flora of healthy women, play important roles to prevent bacterial vaginosis and other sexually transmitted diseases. In this study, we carried out studies on stress adaptation prior to various stress treatment. We found that heat or salt adapted KLB46 showed higher cell viability than non adapted upon heat stress at $60^{\circ}C$ for 20 min. When chloramphenicol was added during the adaptation process, heat tolerance was abolished. This result suggested that de novo protein synthesis was essential during adaptation.

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참고문헌

  1. Bastani, P., A. Homayouni, V. G. Tabrizian, and S. Ziyadi (2012) Dairy probiotic foods and bacterial vaginosis: A review on mechanism of action. pp. 445-456. In: EC Rigobelo (eds.). Probiotics. InTech.
  2. Chang, C. E. (2000) Isolation and characterization of Lactobacillus spp. from human vagina for the ecological treatment of bacterial vaginosis. M.S. thesis, Dept. of Biological Engineering, Inha University, Incheon, Korea.
  3. Heidebach, T., P. Frst, and U. Kulozik (2012) Microencapsulation of probiotic cells for food applications. Crit Rev Food Sci Nutr. 52: 291-311. https://doi.org/10.1080/10408398.2010.499801
  4. Silva, J., R. Freixo, P. Gibbs, and P. Teixeira (2011) Spray-drying for the production of dried cultures. Int. J. Dairy Tech. 64: 321-335. https://doi.org/10.1111/j.1471-0307.2011.00677.x
  5. Paz, R., L. Lavari, G. Vinderola, G. Audero, A. Cuatrin, N. Zaritzky, and J. Reinheimer (2012) Effect of heat-treatment and spray drying on lactobacilli viability and resistance to simulated gastrointestinal digestion. Food Res Int. 48: 748-754. https://doi.org/10.1016/j.foodres.2012.06.018
  6. Moseley, P. L. (1997) Heat shock proteins and heat adaptation of the whole organism. J. Appl. Physiol. 83: 1413-1417. https://doi.org/10.1152/jappl.1997.83.5.1413
  7. Sharareh, S. B., W. Shengqian, T. Stefan, H. Maria, A. Iris, M. U. Frank, W. Michael, and V. Helmut (2013) Impact of heat treatment and spray drying on cellular properties and culturability of Bifidobacterium bifidum BB-12. Food Res Int. 54: 93-101. https://doi.org/10.1016/j.foodres.2013.05.024
  8. Park, M. Y., H. M. Park, J. S. So, and S. C. Kim (2004) Microbiologic and molecular genetic analysis of Lactobacillus spp. isolated from vagina of Korean women and a pilot clinical study on the treatment of vaginitis using the best Lactobacillus strain KLB46. Korean J. Obstet Gynecol. 47: 1154-1164.
  9. Kim, J. H., S. Y. Lee, C. E. Chang, S. C. Kim, H. S. Yun, and J. S. So (2001) Effect of cold adaptation on the improved viability of Lactobacillus crispatus KLB46. Korean J. Biotechnol. Bioeng. 16: 626-631.
  10. Fiocco, D., V. Capozzi, P. Goffin, P. Hols, and S. Giuseppe (2005) Improved adaptation to heat, cold, and solvent tolerance in Lactobacillus plantarum. Appl. Microbiol Biotechnol. 77: 909-915.
  11. O' Sullivan, E. and S. Condon (1997) Intracellular pH is a major factor in the induction of tolerance to acid and other stresses in lactococcus lactis, Appl. Environ Microbiol. 63: 4210-4215.
  12. Skandamis, P. N., Y. Yoon, J. D. Stopforth, P. A. Kendall, and J. N. Sofos (2008) Heat and acid tolerance of Listeria monocytogenes after exposure to single and multiple sublethal stresses. Food Microbiol. 25: 294-303. https://doi.org/10.1016/j.fm.2007.10.008
  13. Lorca, G. L. and G. F. de Valdez (2009) Lactobacillus stress response. pp. 115-137. In: Ljungh and T Wadstrm (eds.). Lactobacillus Molecular Biology: From Genomics to Probiotics. Calister Academic Press. Norfolk. UK.
  14. Prasad, J., P. McJarrow, and P. Gopal (2003) Heat and osmotic stress responses of probiotic Lactobacillus rhamnosus HN001 (DR20) in relation to viability after drying. Appl. Environ Microbiol. 69: 917-925. https://doi.org/10.1128/AEM.69.2.917-925.2003