DOI QR코드

DOI QR Code

Antioxidative Activity of Cheonggukjang Prepared with Purple Sweet Potato

자색고구마 첨가 청국장의 항산화능 평가

  • Lee, MinJi (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) ;
  • Lee, Yu Geon (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University) ;
  • Cho, Jung-Il (Department of Food Nutrient and Culinary, Chosun College of Science & Technology) ;
  • Na, Kwang-Chool (Department of Food Nutrient and Culinary, Chosun College of Science & Technology) ;
  • Kim, Mi Seung (Muan-gun Agritechnology Center) ;
  • Moon, Jae-Hak (Department of Food Science & Technology, and Functional Food Research Center, Chonnam National University)
  • 이민지 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 이유건 (전남대학교 식품공학과 및 기능성식품연구센터) ;
  • 조정일 (조선이공대학교 식품영양조리과학과) ;
  • 나광출 (조선이공대학교 식품영양조리과학과) ;
  • 김미승 (무안군 농업기술센터) ;
  • 문제학 (전남대학교 식품공학과 및 기능성식품연구센터)
  • Received : 2013.10.13
  • Accepted : 2014.01.09
  • Published : 2014.04.30

Abstract

The characteristics and antioxidative activities of yellow and black soybean cheonggukjang prepared with the addition of purple sweet potato (Ipomoea batatas cv. Jami) were evaluated. In sensory evaluation experiments involving the addition of purple sweet potatoes to cheonggukjang at 0, 5, 10, 20, and 30% ratios (w/w), yellow (YCAP) and black (BCAP) soybean cheonggukjang with 20% purple sweet potato were most preferred. The total phenolics and flavonoid contents of YCAP and BCAP were higher than those of yellow (YC) and black (BC) cheonggukjang prepared without added purple sweet potato. YCAP and BCAP also showed higher DPPH radical-scavenging activities than other samples. Moreover, rat blood plasma samples taken 1 h after oral administration of YCAP and BCAP showed higher inhibitory effects against lipid peroxidation than did rats fed YC or BC. These results suggest that the long-term intake of cheonggukjang prepared with purple sweet potato may increase the antioxidative activity in the blood.

노랑콩과 검정콩 각각을 이용하여 자색고구마를 첨가한 청국장을 제조 후, 그 함유성분의 특성 분석과 기능성 평가를 위한 in vitro 및 동물실험을 행하였다. 관능평가 결과, 자색고구마의 최적 첨가 비율은 노랑콩과 검정콩 모두 원료 콩 중량비의 20%인 것으로 나타났다. 그래서 자색고구마 20%를 첨가하여 제조한 청국장과 자색고구마를 첨가하지 않은 노랑콩 청국장 및 검정콩 청국장을 대상으로 그들의 특성을 비교한 결과, 자색고구마 첨가군이 자색고구마 무첨가군보다 총 페놀성 및 flavonoid 함량에 있어 유의하게 더 높은 결과를 나타냈다. 또한 발효를 행하지 않은 자색고구마 첨가 시료들과 비교하였을 때에도 발효를 행한 시료들이 보다 더 높은 함량을 나타냈다. 그리고 DPPH radical-scavenging 활성을 비교한 결과에서도 자색고구마 첨가 청국장의 실험군이 자색고구마 무첨가 청국장 군들에 비해 더 높은 DPPH radical-scavenging 효과를 나타냈다. 또 동이온 유도산화에 따른 쥐 혈장 산화억제능 평가에 있어서도 자색고구마를 첨가하여 제조한 청국장 추출물을 투여한 군의 혈장이 청국장 추출물을 투여하지 않은 대조군과 자색고구마를 첨가하지 않은 일반 청국장 추출물을 투여한 쥐 혈장들에 비해 산화가 더 효과적으로 억제됨을 알 수 있었다. 이상의 결과들은 자색고구마에 함유된 anthocyan 등의 유효성분 및 발효과정 중에 생성된 대사산물에 의해 생리활성이 향상된 것으로 판단되며, 자색고구마의 유용성 확대 및 청국장 제조산업에 유용한 자료로 활용되길 기대한다.

Keywords

References

  1. Lee YL, Kim SH, Jung NH, Lim MH. A study on the production of viscous substance during the chungkookjang fermentation. J. Korean Soc. Agric. Chem. Biotechnol. 35: 202-209 (1992)
  2. Joo EY, Park CS. Antioxidant and fibrinolytic activities of extracts from soybean and chungkukjang (fermented soybean paste). Korean J. Food Preserv. 18: 930-937 (2011) https://doi.org/10.11002/kjfp.2011.18.6.930
  3. Okamato A, Hanagata H, Matsumoto E, Kawamura T, Koizume Y, Yanagida F. Angiotensin I converting enzyme inhibitory activities of various fermented foods. Biosci. Biotech. Bioch. 59: 1147-1149 (1995) https://doi.org/10.1271/bbb.59.1147
  4. Kim US, Kim JY, Kim SJ, Moon KH, Baek SH. Isoflavone contents, antibacterial activities, and physiological activities of cheonggukjang made from sword bean. J. Korean Soc. Food Sci. Nutr. 41: 174-181 (2012) https://doi.org/10.3746/jkfn.2012.41.2.174
  5. Min HK, Kim HJ, Chang HC. Growth-inhibitory effect of the extract of porphyran-chungkookjang on cancer cell. J. Korean Soc. Food Sci. Nutr. 37: 826-833 (2008) https://doi.org/10.3746/jkfn.2008.37.7.826
  6. Yoo CK, Seo WS, Lee CS, Kang SM. Purification and characterization of fibrinolytic enzyme excreted by Bacillus subtilits K-54 isolated from chungkookjang. J. Appl. Microbiol. Biotechnol. 26: 506-514 (1998)
  7. Lee BY, Kim DM, Kim KI. Physicochemical properties of viscous substance extracted from chungkookjang. Korean J. Food Sci. Technol. 23: 599-604 (1991)
  8. Park JS, Cho SH, Na HW. Properties of cheongkukjang prepared with admixed medicinal herb powder. Korean J. Food Preserv. 17: 343-350 (2010)
  9. Jung YK, Lee YK, No HK, Kim SD. Effect of chitosan on quality characteristics of chungkukjang. J. Korean Soc. Food Sci. Nutr. 35: 476-481 (2006) https://doi.org/10.3746/jkfn.2006.35.4.476
  10. Kim JH, Kim SI, Kim JG, Im, DK, Park JG, Lee JW, Byun MW. Effect of green tea powder on the improvement of sensorial quality of chungkookjang. J. Korean Soc. Food Sci. Nutr. 35: 482- 486 (2006) https://doi.org/10.3746/jkfn.2006.35.4.482
  11. Jeong YJ, Woo SM, Kwon JH, Choi MS, Seong JH, Lee JW. Quality characteristics of red ginseng cheonggukjang according to addition methods of red ginseng. J. Korean Soc. Food Sci. Nutr. 36: 889-895 (2007) https://doi.org/10.3746/jkfn.2007.36.7.889
  12. In JP, Lee SK, Ahn BK, Chung IM, Jang CH. Flavor improvement of chungkookjang by addition of yucca (yucca shidigera) extract. Korean J. Food Sci. Technol. 34: 57-64 (2002)
  13. Rhim JW, Lee JW, Jo Js, Yeo KM. Pilot plant scale extraction and concentration of purple-fleshed sweet potato anthocyanin pigment. Korean J. Food Sci. Technol. 33: 808-811 (2001)
  14. Kim SY, Ryu CH. Studies on nutritional components of purple sweet potato (Ipomoea batatas). Korean J. Food Sci. Technol. 27: 819-825 (1995)
  15. Choi EJ, Lee JH. Quality and antioxidant properties of jelly incorporated with purple sweet potato concentrate. Korean J. Food Sci. Technol. 45: 47-52 (2013) https://doi.org/10.9721/KJFST.2013.45.1.47
  16. Kim SY, Ryu CH. Effect of certain additives on bread-marking quality of wheat-purple sweet potato flours. Koran J. Food Sci. Technol. 13: 492-499 (1995)
  17. Cho HK, Lee JY, Seo WT, Kim MK, Cho KM. Quality characteristics and antioxidant effects during makgeolli fermentation by purple sweet potato-rice nuruk. Koran J. Food Sci. Technol. 44: 728-735 (2012) https://doi.org/10.9721/KJFST.2012.44.6.728
  18. Ahn GJ. Quality characteristics of sulgidduk prepared with amount of purple sweet-potato powder. Korean J. Culinary Res. 16: 127-136 (2010)
  19. Record IR, Dreosit IE, Mclnerney JK. The antioxidant activity of genistein in vitro. J. Nutr. Biochem. 6: 481-485 (1995) https://doi.org/10.1016/0955-2863(95)00076-C
  20. Wei H, Cai Q, Rahn R. Inhibition of UV light and fenton reaction-induced oxidative DNA damage by the soybean isoflavone genistein. Carcinogenesis 17: 73-78 (1996) https://doi.org/10.1093/carcin/17.1.73
  21. Kim HE, Han SY, Jung JB, Ko JM, Kim YS. Quality characteristics of doenjang (soybean paste) prepared with germinated regular soybean and black soybean. Korean J. Food Sci. Technol. 43: 361-368 (2011) https://doi.org/10.9721/KJFST.2011.43.3.361
  22. Shon MY, Seo KI, LEE SW, Choi SH, Sung NJ. Biological activities of chungkugjang prepared with black bean and changes in phytoestrogen content during fermentation. Korean J. Food Sci. Technol. 32: 936-941 (2000)
  23. Cho JI, Cho SH, Cho SH. Soybean food product fermented by Bacillus subtilis chungkook16. Repub. Korea patent 10-2006-0072105 (2006)
  24. Singleton VL, Rossi JA, Colorimetry of total phenolics with phospomolybdic-phosphotungstic acid reagents. Amer. J. Enol. Viticult. 16: 144-158 (1965)
  25. Lee YC, Hwang KH, Han DH, Kim SD. Compositions of Opuntia ficus-indica. Korean J. Food Sci. Technol. 29: 847-853 (1997)
  26. Yamaguchi T, Takamura H, Matoba T, Terao J. HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl. Biosci. Biotechnol. Bioch. 62: 1201-1204 (1998) https://doi.org/10.1271/bbb.62.1201
  27. Kim GD, Lee YS, Cho JY, Lee YH, Choi KJ, Lee Y, Han TH, Lee SH, Park KH, Moon JH. Comparision of the content of bioactive substances and the inhibitory effects against rat plasma oxidation of conventional and organic hot pepper (Capsicum annuum L.). J. Agr. Food Chem. 58: 12300-12306 (2010) https://doi.org/10.1021/jf1028448
  28. Kim JH, Kim SI, Kim JG, Im DK, Park JG, Lee JW, Byun MW. Effect of green tea powder on the improvement of sensorial quality of chungkookjang. J. Korean Soc. Food Sci. Nutr. 35: 482-486 (2006) https://doi.org/10.3746/jkfn.2006.35.4.482
  29. Kim JM, Park SJ, Lee CS, Ren C, Kim SS, Shin MS. Functional properties of different Korean sweet potato varieties. Food Sci. Biotechnol. 20: 1501-1507 (2011) https://doi.org/10.1007/s10068-011-0208-1
  30. Wang HJ, Murphy PA. Isoflavone content in commercial soybean foods. J. Agr. Food Chem. 42: 1666-1673 (1994) https://doi.org/10.1021/jf00044a016
  31. Goldstein JL, Ho YK, Brown MS. Binding site on macrophages that mediates uptake and degradation of acetylated low density lipoprotein, producing massive cholesterol deposition. Proc. Natl. Acad. Sci. USA 76: 333-337 (1979) https://doi.org/10.1073/pnas.76.1.333

Cited by

  1. Substitution Effect of Enzymatically Hydrolyzed Purple Sweet Potato Powder on Skim Milk in Yogurt Preparation vol.58, pp.4, 2015, https://doi.org/10.3839/jabc.2015.049
  2. Single Dose Oral Toxicity of Bacillus subtilis JNS in ICR Mice vol.44, pp.1, 2015, https://doi.org/10.3746/jkfn.2015.44.1.024