Journal of the Korea Academia-Industrial cooperation Society (한국산학기술학회논문지)

The Korea Academia-Industrial cooperation Society (한국산학기술학회)
권호 표지
DOI QR코드

DOI QR Code

The prevalence of Extended Spectrum β-Lactamase type produced by Clinical Isolates of Escherichia coli from ChungCheong Area

충청 지역에서 분리된 대장균이 생성하는 Extended Spectrum β-Lactamase 유형의 검출 빈도

  • Yook, Keun-Dol (Dept. of Clinical Laboratory Science, Deajeon Health Science College) ;
  • Park, Jin-Sook (Dept. of Biological Sciences and Biotechnology, Hannam University)
  • 육근돌 (대전보건대학교 임상병리과) ;
  • 박진숙 (한남대학교 생명시스템과학과)
  • Received : 2014.03.19
  • Accepted : 2014.04.10
  • Published : 2014.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

The study aims primarily to evaluate the resistance of antibiotics and the prevalence of these enzymes among Escherichia coli the most frequent isolate of Enterobacteriaceae producing Extended-Spectrum ${\beta}$-Lactamase(ESBLs), to differentiate the types of enzymes in these isolates. Total 74(26.2%) Strains of producing ESBLs among the 282 E. coli isolates were isolated from hospitals of Chungcheong area (Daejeon, Chungnam, and Chungbuk) during a 6 month-period from February to July, 2013. 282 E. coli isolates including ESBL shown resistance rates of aztreonam 30.8%, Cefotaxim 30.9%, and Ceftazidime 32.2%, 74 isolates producing ESBLs in E. coli were resistant rates to Aztreonam 58.1%, Cefotaxim 100%, and Ceftazidime 63.5% of ${\beta}$-lactam antibiotics. CTX-M-2 (48 isolates) was the most prevalent type of ESBLs identified. Followed the order of frequency by PER-1 (28 isolates), VEB-1 (26 isolates) and CTX-M-8 (20 isolates), of the 74 isolates, 2 isolates only showed GES-1 in Chungnam province. Accurate identification type of ESBLs would aid in hospital infection control. This would give aid to the physician to prescribe more appropriate antibiotics.

장내세균 가운데서 가장 빈번하게 나오는 대장균에서 Extended-Spectrum ${\beta}$-Lactamase(ESBL)를 생성하는 효소의 검출빈도와 이들 균주의 ESBL 효소의 유형을 구분하는 것이 이 연구의 목적이다. 균주는 충청지역 (대전, 충남, 충북)병원에서 2013년 2월부터 7월까지 6개월간 282 대장균 균주 중 ESBL을 생성하는 74균주(26.2%)를 수집하였다. 충청지역에 ESBL 균주를 포함한 대장균의 항균제 내성률은 Aztreonam 30.8%, Cefotaxim 30.9%, 그리고 Ceftazidime 32.2%로 나타났으며 ESBL을 생성하는 균주는 ${\beta}$-lactam 항균제인 Aztreonam에 58.1%, Cefotaxim 100%, 그리고 Ceftazidime 63.5%의 내성률을 보였다. 동정된 ESBL 균의 CTX-M-2은 48 균주, CTX-M-8은 20 균주, PER-1 28 균주, 그리고 VEB-1 26 균주에서 나왔다. GES-1 형은 74균주 중 2균주가 충남에서만 유일하게 나타났다. ESBL 검출빈도와 유형의 정확한 파악은 병원감염관리와 항균제 처방에 도움이 될 것이다.

Keywords

References

  1. Shin KS and Son BR. Comparison of Vitek ESBL test and other methods for detecting extended-spectrum $\beta$- lactamase producing Escherichia coli and Klebsiella Species. Korean J Clin Pathol; 22:21-6, 2002.
  2. Hong SG, Kang MS, Choi JR, Lee KW, Chong YS, Kwon OH. Molecular characteristics of extended-spectrum $\beta$-lactamases in clinical isolates of Enterobacteriaceae. Korean J Clin Pathol; 21:495-504, 2001.
  3. Lee BY, Jeong SH, Jeong TS, Nam HJ, Ji JH, Hong YR. Detection of extended-spectrum $\beta$-lactamase producing Escherichia coli and Klebsiella spp. with the Vitek GNS 121 card. Korean J Clin Pathol; 21:350-4, 2001.
  4. Edelstein M, Pimkin M, Palagin I, Edelstein I, and Stratchounski L :Prevalence and Molecular Epidemiology of CTX-M Extended-Spectrum $\beta$-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Russian Hospitals. Antimicrob Agents Chemother, 47: 3724-3732, 2003. DOI: http://dx.doi.org/10.1128/AAC.47.12.3724-3732.2003
  5. Bush K (2001): New $\beta$-lactamases in Gram-negative bacteria: diversity and impact on the selection of antimicrobial Gaussard S and Courvalin P (1999) : Updated sequence information TEM $\beta$-lactamase genes. Antimicrob Agents Chemother, 43: 367-370. herapy. Clin Infect Dis, 32: 1085-1089. https://doi.org/10.1086/319610
  6. Gaussard S and Courvalin P : Updated sequence information TEM $\beta$-lactamase genes. Antimicrob Agents Chemother, 43: 367-370,1999. https://doi.org/10.1093/jac/43.3.367
  7. Bradford PA. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev ;14:933-51, 2001. DOI: http://dx.doi.org/10.1128/CMR.14.4.933-951.2001
  8. Jarlier V, Nicolas MH, Fournier G, Philippon A. Extended broadspectrum $\beta$-lactamases conferring transferable resistance to newer $\beta$-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev Infect Dis. 1988; 10: 867-78. DOI: http://dx.doi.org/10.1093/clinids/10.4.867
  9. Pai HJ. The characteristics of extended-spectrum $\beta$ -lactamases in Korean isolates of Enterobacteriaceae. Yonsei Med J 1998; 39: 514-9. https://doi.org/10.3349/ymj.1998.39.6.514
  10. Steward CD, Rasheed K, Hubert SK, Biddle JW, Raney PM, Anderson GJ, et al. Characterization of clinical isolates of Klebsiella pneumoniae from 19 laboratories using the National Committee for Clinical Laboratory Standards extended-spectrum $\beta$-lactamase detection methods. J Clin Microbiol ; 39: 2864-72, 2001. DOI: http://dx.doi.org/10.1128/JCM.39.8.2864-2872.2001
  11. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement. M100-S16. Wayne, Pensylvania: CLSI, 2006.
  12. Jones RN, Pfaller MA, Doern GV, Erwin ME, Hollis RJ. Antimicrobial activity and spectrum investigation of eight broad-spectrum beta-lactam drugs: a 1997 surveillance trial in 102 medical centers in the United States. Cefepime Study Group. Diagn Microbiol Infect Dis ;30:215-28, 1998. DOI: http://dx.doi.org/10.1016/S0732-8893(97)00234-4
  13. Park JH, Lee SH, Jeong SH, Kim BN, Kim KB, Yoon JD, et al. Characterization and prevalence of Escherichia coli and Klebsiella pneumoniae isolates producing an extended-spectrum $\beta$-lactamase from Korean hospitals. Korean J Lab Med; 23:18-24, 2003.
  14. Lee JH, Bae IK, Kwon SB, Jeong SH, Woo GJ, Lee JW, et al. Prevalence of CTX-M-type extended-spectrum $\beta$ -lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates in Korea, 2003. Korean J Clin Microbiol; 7:111-8, 2004.
  15. Chi Seon Ko, Ji Yun Sung, Sun Hoe Koo, et al.,Prevalence of Extended-Spectrum $\beta$-lactamases in Escherichia coli and Klebsiella pneumoniae from Daejeon: Korean J Lab Med 2007;27:344-50. DOI: http://dx.doi.org/10.3343/kjlm.2007.27.5.344
  16. Kang JH, Bae IK, Kwon SB, Jeong SH, Lee JW, Lee WG, et al. Prevalence of Ambler Class A extended-spectrum $\beta$-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates in Korea. Korean J Clin Microbiol ;8:17-25, 2005.
  17. Walther-Rasmussen, J., and N. Hoiby. Class A carbapenemases. J. Antimicrob. Chemother. 60:470-482, 2007. DOI: http://dx.doi.org/10.1093/jac/dkm226
  18. Xavier DE, Picao RC, Girardello R, Fehlberg LC, Gales AC.Efflux pumps expression and its association with porin down-regulation and beta-lactamase production am ong Pseudomonas aeruginosa causing bloodstream infec tions in Brazil.BMC Microbiol. 2010 Aug 12;10:217. https://doi.org/10.1186/1471-2180-10-217
  19. Ranellou K, Kadlec K, Poulou A, et al., Detection of Pseudomonas aeruginosa isolates of the international clonal complex 11 carrying the blaPER-1 extended-spectrum b-lactamase gene in Greece, J Antimicrob Chemother 2012; 67:357 361 DOI: http://dx.doi.org/10.1093/jac/dkm226
  20. Kim JM, Kang HK, Jeong SH et al. Prevalence of PER-1 Extended-Spectrum $\beta$-Lactamase-Producing Clinical Isolates of Acinetobacter baumannii in a University Hospital, Busan, Korea: Korean J Clin Microbiol; 7(1): 20-26, 2004.
  21. Laurent Poirel, Thierry Naas, Michele Guibert, et al., Molecular and Biochemical Characterization of VEB-1, a Novel Class A Extended-Spectrum $\beta$-Lactamase Encoded by an Escherichia coli Integron Gene. Antimicrob. Agents Chemother. March 1999 vol. 43 no.3 573-581