한국환경보건학회지 (Journal of Environmental Health Sciences)

  • 제40권2호
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  • Pages.114-126
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  • 2014
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  • 1738-4087(pISSN)
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  • 2233-8616(eISSN)
한국환경보건학회 (Korean Society of Environmental Health)
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HPLC-MS/MS를 이용한 트리클로로에틸렌 대사산물의 다중 분석법 확립

Multiple Determinations of Trichloroethylene Metabolites in a Concurrent Biological Media using High Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry

  • 안영아 (서울대학교 보건대학원 환경보건학과) ;
  • 고영림 (을지대학교 보건환경안전학과) ;
  • 이승호 (서울대학교 보건대학원 환경보건학과) ;
  • 신미연 (서울대학교 보건대학원 환경보건학과) ;
  • 전중대 (을지대학교 보건환경안전학과) ;
  • 김성균 (서울대학교 보건대학원 환경보건학과)
  • Ahn, Youngah (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Kho, Younglim (Department of Health, Environment and Safety, Eulji University) ;
  • Lee, Seungho (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Shin, Mi-Yeon (Department of Environmental Health, Graduate School of Public Health, Seoul National University) ;
  • Jeon, Jung Dae (Department of Health, Environment and Safety, Eulji University) ;
  • Kim, Sungkyoon (Department of Environmental Health, Graduate School of Public Health, Seoul National University)
  • 투고 : 2014.02.03
  • 심사 : 2014.04.14
  • 발행 : 2014.04.30
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초록

Objectives: We aimed to develop a measurement method of five metabolites of trichloroethylene (TCE) in a concurrent biological sample, e.g., trichloroacetic acid (TCA), dichloroacetic acid (DCA), S-(1,2-dichlorovinyl) glutathione (DCVG), S-(1,2-dichlorovinyl)-L-cysteine (DCVC), and N-Acetyl-S-(1,2-dichlorovinyl)-L-cysteine (NAcDCVC) and to validate the method before application to pharmacokinetic study. Methods: TCE metabolites were simultaneously analyzed using high performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS) with as little as 50 ${\mu}L$ of serum and urine. DCA, TCA and NAcDCVC were extracted with diethyl ether, while DCVC and DCVG were extracted by solid phase extraction. This method was validated according to the guidelines for bioanalytical method validation of the Korean National Institute of Toxicological Research. Then, we determined the five metabolites in five strains of mice at 24 hr after exposure to 1 g TCE /kg body weight. Results: The limits of detection for the five metabolites in biological samples ranged from 0.001 to 0.076 nmol/mL, which is comparable to or better than those previously reported. Most calibration curves showed good linearity ($R^2=0.99$), and between-batch variation was less than 20% expressing acceptable robustness and reproducibility. Using this method, we found TCA and DCA were detected in all test mice at 24 hr after the oral administration while NAcDCVC and DCVC were detected in some strains, which showed strain-dependent metabolism of TCE. Conclusions: The present method could provide robust and accurate measurements of major key metabolites of TCE in biological media, which allowed concurrent analysis of TCE metabolism for limited amounts of biospecimens.

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참고문헌

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