Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

  • Kim, Hyun A (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Biosystems and Bioengineering Program, University of Science and Technology) ;
  • Kwon, Suk Yoon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Biosystems and Bioengineering Program, University of Science and Technology) ;
  • Yang, Moon Sik (Division of Biological Science, Chonbuk National University) ;
  • Choi, Pil Son (Department of Medicinal Plant Resources, Nambu University)
  • Received : 2014.03.19
  • Accepted : 2014.03.26
  • Published : 2014.03.31
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Abstract

Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two- and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.

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References

  1. Amstrong CL, Green CE Phillips RL (1991) Development and availability of germplasm with high type II culture formation response. Maize Genetics Cooperative newsletter 65:92-93
  2. Chang GJ (1997) Molecular biology of dengue viruses. Gubler DJ, Kuno G (Eds.), Dengue and Dengue Hemorrhagic Fever, The University press, Cambridge, United Kingdom 175-198
  3. Cho MA, Kwon SY, Kim JS, Lee BK, Moon CY, Choi PS (2007) Expression of CP4 5-enol-pyruvylshikimate-3-phosphate synthase transgene inbred line of Korean domestic maize (Zea may L.). Kor J Plant Biotechnol 34(4):375-380 https://doi.org/10.5010/JPB.2007.34.4.375
  4. Cho MA, Park UO, Kim JS, Park KJ, Min HK, Liu JR, Clemente T, Choi PS (2005) Production of transgenic maize (Zea mays L.) using Agrobacterium tumefaciens-mediated transformation. Kor J Plant Biotechnol 32(2):91-95 https://doi.org/10.5010/JPB.2005.32.2.091
  5. Crill WD, Roehrig JT (2001) Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to Vero cells. J Virol 75:7769-7773 https://doi.org/10.1128/JVI.75.16.7769-7773.2001
  6. Dellaporta SL, Wood J, Hicks JB (1983) Maize DNA miniprep. In: Malmberg R, Messing J, Sussex (eds), Molecular Biology of Plants: A laboratory Course Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. pp36-37
  7. Frame BR, Shou H, Chikwamba RK, Zhang Z, Xiang C, Fonger TM, Pegg SEK, Li B, Nettleton DS, Pei D, Wang K (2002) Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system. Plant Physiol 129:13-22 https://doi.org/10.1104/pp.000653
  8. Gaba V, Zelcer A, Gal-On A (2004) Cucurbit biotechnology-the importance of virus resistance. In Vitro Cell Dev Biol-Plant 40:346-358 https://doi.org/10.1079/IVP2004554
  9. Jones JDG, Gilbert DE, Grady KL, Jorgensen RA (1987) T-DNA structure and gene expression in petunia plants transformed by Agrobacterium C58 derivatives. Mol General Genet 207:478-485 https://doi.org/10.1007/BF00331618
  10. Kim HA, Yoo HS, Yang MS, Kwon SY, Kim JS, Choi PS (2010) The development of transgenic maize expressing Actinobacillus pleuropneumoniae APXIIA gene using Agrobacterium. Kor J Plant Biotechnol 37(3):313-318 https://doi.org/10.5010/JPB.2010.37.3.313
  11. Kim HA, Utomo SD, Kwon SY, Min SR, Kim JS, Yoo HS, Choi PS (2009) The development of herbicide-resistant maize: stable Agrobacterium-mediated transformation of maize using explants of type II embryogenic calli. Plant Biotechnol Rep 3:277-283 https://doi.org/10.1007/s11816-009-0099-2
  12. Kim TG, Kim MY, Yang MS (2010) Cholera toxin B subunit-domain III of dengue virus envelope glycoprotein E fusion protein production in transgenic plants. Protein Expres Purif 74:236-241 https://doi.org/10.1016/j.pep.2010.07.013
  13. Koncz C, Martini N, Mayerhofer R, Koncz KZ, Korber H, Redei GP, Schell J (1989) High-frequency T-DNA-mediated gene tagging in plants. Proc Natl Acad Sci USA 86:8467-8471 https://doi.org/10.1073/pnas.86.21.8467
  14. Mason HS, Ball JM, Shi JJ, Jiang X, Estes MK, Arntzen CJ (1996) Expression of norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice. Proc Natl Acad Sci USA 93:5335-5340 https://doi.org/10.1073/pnas.93.11.5335
  15. Mason HS, Dominic Mam-Kit L, Arntzen CJ (1992) Expression of hepatitis B surface antigen in transgenic plants. Proc Natl Acad Sci USA 89:11445-11749
  16. Mason HS, Haq TA, Clements JD, Arntzen CJ (1998) Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): potatoes expressing a synthetic LT-B gene. Vaccine 16:1336-1343 https://doi.org/10.1016/S0264-410X(98)80020-0
  17. Mukhopadhyay S, Kuhn RJ, Rossmann MG (2005) A structural perspective of the flavivirus life cycle. Nat Rev Microbiol 3:13-22 https://doi.org/10.1038/nrmicro1067
  18. Prols F, Meyer P (1992) The methylation patterns of chromosomal integration regions influence gene activity of transferred DNA in Petunia hybrid. Plant J 2:465-475
  19. Saejung W, Fujiyama K, Takasaki T, Ito M, Hori K, Malasit P, Watanabe Y, Kurane I, Seki T (2007) Production of dengue 2 envelope domain III in plant using TMV-based vector system. Vaccine 25:6646-6654 https://doi.org/10.1016/j.vaccine.2007.06.029
  20. Seema S, Jain K (2005) Molecular mechanism of pathogenesis of Dengue virus : Entry and fusion with target cell. Indian J Clinic Biochem 20:92-103 https://doi.org/10.1007/BF02867407
  21. Shin MK, Jung MH, Lee WJ, Choi PS, Jang YS, Yoo HS (2011) Generation of transgenic corn-derived Actinobacillus pleuropneumoniae APXIIA fused with the cholera toxin B subunit as a vaccine candidate. J Vet Sci 12(4):401-403 https://doi.org/10.4142/jvs.2011.12.4.401
  22. Stam M, Mol JNM, Kooter JM (1997) The silence of genes in transgenic plants. Anals Bot 79:3-12

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