Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Simultaneous Determination and Optimization Ultrasound-Assisted Extraction of Poncirin and Naringin in Poncirus trifoliata Rafinesqul

지실의 Poncirin, Naringin의 동시분석법 확립과 초음파 추출법 최적화

  • Lee, Ah Reum (Herbal Resources Group, Korea Institute of Oriental Medicine) ;
  • Jang, Seol (Herbal Resources Group, Korea Institute of Oriental Medicine) ;
  • Lee, A Yeong (Herbal Resources Group, Korea Institute of Oriental Medicine) ;
  • Choi, Goya (Herbal Resources Group, Korea Institute of Oriental Medicine) ;
  • Kim, Hyo Seon (Herbal Resources Group, Korea Institute of Oriental Medicine) ;
  • Kim, Ho Kyoung (Herbal Resources Group, Korea Institute of Oriental Medicine)
  • 이아름 (한국한의학연구원 한약자원그룹) ;
  • 장설 (한국한의학연구원 한약자원그룹) ;
  • 이아영 (한국한의학연구원 한약자원그룹) ;
  • 최고야 (한국한의학연구원 한약자원그룹) ;
  • 김효선 (한국한의학연구원 한약자원그룹) ;
  • 김호경 (한국한의학연구원 한약자원그룹)
  • Received : 2014.02.12
  • Accepted : 2014.03.24
  • Published : 2014.04.30
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Abstract

The Ponciri fructus immaturus (Poncirus trifoliata Rafinesque) has been used in oriental medicine for uterine contraction, stomachache, abdominal distension and cardiovascular diseases. Two main compounds, poncirin and naringin were successfully analyzed by high performance liquid chromatography (HPLC) and carried out method validation according to ICH guideline. A successful resolution and retention times were obtained with a $C_{18}$ reversed phase column, at an $1m{\ell}min^{-1}$ flow rate, with a gradient elution of a mixture of methanol, water and acetonitrile. Poncirin and naringin showed good linearity ($R^2$ > 0.999) in relatively wide concentration ranged. The recovery of each compound was 95.81 ~ 101.48% with R.S.D. values less than 1.0%. The application of ultrasound-assisted extraction was shown to be more efficient in extracting poncirin and naringin from Ponciri fructus immaturus. The predicted optimal poncirin and naringin yield were poncirin 2.15%, naringin 1.65% under an extraction temperature of $40^{\circ}C$, an extraction time of 10 min in a solvent of 70% methanol.

Keywords

References

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