한국어병학회지 (Journal of fish pathology)

  • 제27권1호
  • /
  • Pages.11-16
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  • 2014
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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Resistance against white spot syndrome virus (WSSV) infection in wild marine crab Gaetice depressus by injection of recombinant VP28 protein

  • Kim, Chun Soo (Food Standard Division, Ministry of Food and Drug Safety) ;
  • Choi, Seung Hyuk (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Min Sun (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Ki Hong (Department of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2014.02.14
  • 심사 : 2014.04.11
  • 발행 : 2014.04.30
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초록

The resistance against white spot syndrome virus (WSSV) infection in wild marine crab Gaetice depressus by the immunization of a recombinant glutathione-S-transferase (GST) fused VP28 protein (GST-VP28) was evaluated. The cumulative mortalities of GST-VP28 injected groups were lower than those of the control groups at 10 days of post-challenge, and the time to death of 50% crab ($TD_{50}$) was delayed by the immunization using GST-VP28. The group boosted with GST-VP28 after 2 weeks of primary immunization clearly showed longer $TD_{50}$ than non-boosted group against challenge with WSSV. This result suggests that boosting with the antigen protein elicit stronger immune responses similar to adaptive immune responses of vertebrates. However, the short $TD_{50}$ was observed in the group challenged at 3 weeks post boosting comparing to the group challenged at 1 week post boosting. This suggests that the protective strength of immunization decreased by the time.

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참고문헌

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