한국균학회지 (The Korean Journal of Mycology)

  • 제42권1호
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  • Pages.79-85
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  • 2014
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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태안반도에 자생하는 해안식물 뿌리에서 분리한 내생진균의 다양성 분석

Diversity Analysis of Endophytic Fungi Isolated from the Roots of Coastal Plants in Taean Peninsula

  • You, Young-Hyun (School of Life Science, Kyungpook National University) ;
  • Yoon, Nam-Gyeong (School of Life Science, Kyungpook National University) ;
  • Yoon, Hyeokjun (School of Life Science, Kyungpook National University) ;
  • Kim, Hyun (School of Life Science, Kyungpook National University) ;
  • Lim, Sung Hwan (Department of Biology, Kyungpook National University) ;
  • Choo, Yeon-Sik (Department of Biology, Kyungpook National University) ;
  • Kim, Jong-Guk (School of Life Science, Kyungpook National University)
  • 투고 : 2014.03.06
  • 심사 : 2014.03.17
  • 발행 : 2014.03.31
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초록

태안반도에 자생하는 해안식물인 해국(Aster sphathulifolius Maxim.), 갯개미취(Aster tripolium L.), 갈대(Phragmites australis), 갯꾸러미풀(Puccinellia nipponica Ohwi) 의 뿌리로부터 총 42주의 내생진균이 분리되었다. 그리고 분리된 내생진균의 ITS지역의 염기서열을 이용하여 동정한 결과 모두 자낭균문에 속하며, 8종류의 목(Botryosphaeriales, Capnodiales, Diaporthales, Dothideales, Eurotiales, Helotiales, Hypocreales, and Pleosporales)과 13종류의 속(Alternaria, Aureobasidium, Cadophora, Cladosporium, Davidiella, Diaporthe, Fusarium, Gibberella, Macrophomina, Metarhizium, Neosartorya, Penicillium, and Phoma)으로 분류되었으며, Eurotiales목의 Penicillium속의 분포비율이 가장 높게 존재하였다. Shannon 다양성지수를 비교한 결과 4종의 해안식물 중 갯개미취에서 분리한 내생진균의 다양성이 가장 높게 확인되었다.

Forty-two endophytic fungi were isolated from the roots of coastal plants, such as Aster sphathulifolius Maxim., Aster tripolium L., Phragmites australis, and Puccinellia nipponica Ohwi, naturally growing in Taean peninsula. The identity of the endophytic fungal strains was analyzed using the sequence of internal transcribed spacer regions. All fungi belonged to the phylum Ascomycota, and they were classified into eight orders (Botryosphaeriales, Capnodiales, Diaporthales, Dothideales, Eurotiales, Helotiales, Hypocreales, and Pleosporales) and thirteen genera (Alternaria, Aureobasidium, Cadophora, Cladosporium, Davidiella, Diaporthe, Fusarium, Gibberella, Macrophomina, Metarhizium, Neosartorya, Penicillium, and Phoma). Among the analyzed fungi, the fungi belonging to the genus Penicillium in Eurotiales were the most widely distributed. The host plant Aster tripolium L. was found to contain the most diverse endophytic fungal species among the coastal plants.

키워드

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