The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Diversity of Yeasts from Fruits and Flowers of Orchard in Sinam-myeon of Yesan-gun, Chungcheongnam-do, Korea

충남 예산군 신암면 일대 과수원의 과일과 꽃들로부터 효모의 분리 및 분포 특성

  • Hyun, Se-Hee (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Jong-Guk (Chungnam Agricultural Research and Extension Services) ;
  • Park, Won-Jong (Department of Food Technology, Kongju National University) ;
  • Kim, Ha-Kun (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 현세희 (배재대학교 바이오.의생명공학과) ;
  • 이종국 (충남농업기술원) ;
  • 박원종 (공주대학교 식품공학과) ;
  • 김하근 (배재대학교 바이오.의생명공학과) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Received : 2014.03.07
  • Accepted : 2014.03.19
  • Published : 2014.03.31
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Abstract

Several kinds of yeasts from fruits and flowers of orchard in Yesan-gun of Chungcheongnam-do, Korea were isolated and identified by comparison of nucleotide sequences for PCR-amplified D1/D2 region of 26S rDNA using BLAST. Fourty eight yeast strains of twenty five species and one hundred eight yeast strains of fourty eight species were isolated from fruits and flowers of orchard in Sinam-myeon of Yesan-gun, Chungcheongnam-do, respectively. Among total one hundred fifty-six yeast strains, only sixteen species were overlapped from fruits and flowers.

충청남도 예산군 신암면 일대 과수원들의 과일과 꽃에서 효모들을 분리한 후 이들의 26S rDNA의 D1/D2 부위의 염기서열을 결정한 다음 NCBI의 BLAST 데이터베이스에 등록되어 있는 효모들과의 분자생물학적 유연관계를 비교, 분석하여 동정하였다. 2013년 4월부터 10월 사이의 과일 19점으로부터 모두 25종의 효모들을 48균주 분리하였고 Pichia kluyveri, Hanseniaspora uvarum, Rhodotorula mucilaginosa, Torulaspora delbrueckii 등 4종의 효모 등이 많이 분포하고 있었다. 과수원 주위의 꽃 39점에서 48종의 효모 등을 108균주 분리하였고 이 중 Microstroma juglandis와 Pseudozyma aphidis가 가장 많이 분리되었다.

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References

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