Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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Development of Cost-Effective Platform for Tracking and Analysis of Animal Ambulatory Patterns

  • Kwon, Jeonghoon (Department of Biomedical Engineering, University of Ulsan College of Medicine) ;
  • Park, Hong Ju (Department of Otolaryngology-Head and Neck Surgery, Asan Medical Center) ;
  • Joo, Segyeong (Department of Biomedical Engineering, University of Ulsan College of Medicine) ;
  • Huh, Soo-Jin (Department of Biomedical Engineering, University of Ulsan College of Medicine)
  • Received : 2013.12.24
  • Accepted : 2014.02.26
  • Published : 2014.03.31
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Abstract

This paper reports the development of a platform for tracking and analysis of animal locomotion. The platform is composed of a commercial webcam, a metal stand for the webcam, and a plastic bathtub as a cage. Using it, researchers can track and analyze an animal's movement within the plastic bathtub's dimensions of $100cm{\times}100cm{\times}55cm$ in a cost-effective manner. After recording the locomotion of an animal with $1920{\times}1080$ resolution at a rate of 30 frames per second, finding the position of the animal in each frame and analyzing the ambulation pattern were executed with custom software. To evaluate the performance of the platform, movements of imprinting control region mice and transgenic mice were recorded and analyzed. The analysis successfully compared velocity, moving pattern, and total moving distance for the two mouse groups. In addition, the developed platform can be used not only in simple motion analysis but also in various experimental conditions, such as a water maze, by easy customization of the platform. Such a simple and cost-effective platform yields a powerful tool for animal ambulatory analysis.

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References

  1. J. O. Lim, J. B. Yoo, J. Y. Kwon, H. G. Byun, J. S. Huh, W. J. Cho, "Development of sugar sensitive drosophila cell based ISFET sensor for Alzheimer's disease diagnosis", J. Sensor Sci. & Tech., vol. 22, no. 4, pp. 281-285, 2013. https://doi.org/10.5369/JSST.2013.22.4.281
  2. N. S. Eum, H. J. Park, J. Y. Jung, J. H. Han, H. K. Kim, E. Y. Jang, S. J. Lee, B. H. Kang, and S. W. akng, "Development of small system for mobile-based visible/NIR animal imaging", J. Sensor Sci. & Tech., vol. 21, no. 4, pp. 270-275, 2012. https://doi.org/10.5369/JSST.2012.21.4.270
  3. J. N. Crawley and R. Paylor, "A proposed test battery and constellations of specific behavioral paradigms to investigate the behavioral phenotypes of transgenic and knockout mice", Hormones and Behavior, vol. 31, pp. 197-211, 1997. https://doi.org/10.1006/hbeh.1997.1382
  4. R. V. de Oliveira, O. P. Dall'Igna, A. B. L. Tort, J. F. Schuh, P. F. Neto, M. W. S. Gomes, A. O. Souza, and D. R. Lara, "Effect of subchronic caffeine treatment on MK-801- induced changes in locomotion, cognition and ataxia in mice", Behavioural Pharmacology, vol. 16, pp. 79-84, 2005. https://doi.org/10.1097/00008877-200503000-00002
  5. M. R. M. Vianna, M. Alonso, H. Viola, J. Quevedo, F. Paris, M. Furman, M. L. de Stein, J. H. Medina, and I. Izquierdo, "Role of hippocampal signaling pathways in long-term memory formation of a nonassociative learning task in the rat", Journal of Neurochemistry, vol. 78, pp. 12-12, 2001.
  6. A. Barker, "Role of amyloid beta-protein in Alzheimer's disease", Lancet, vol. 340, pp. 850-851, 1992.
  7. A. Samii, J. G. Nutt, and B. R. Ransom, "Parkinson's disease", Lancet, vol. 363, pp. 1783-93, 2004. https://doi.org/10.1016/S0140-6736(04)16305-8
  8. R. K. Schwarting, R. Goldenberg, H. Steiner, J. Fornaguera, and J. P. Huston, "A video image analyzing system for open-field behavior in the rat focusing on behavioral asymmetries", J Neurosci Methods, vol. 49, pp. 199-210, 1993. https://doi.org/10.1016/0165-0270(93)90125-B
  9. W. H. Pan, C. R. Lee, and L. H. Lim, "A new video path analyzer to monitor travel distance, rearing, and stereotypic movement of rats", J Neurosci Methods, vol. 70, pp. 39-43, 1996. https://doi.org/10.1016/S0165-0270(96)00101-X
  10. E. Ericson, J. Samuelsson, and S. Ahlenius, "Photocell measurements of rat motor activity. A contribution to sensitivity and variation in behavioral observations", J Pharmacol Methods, vol. 25, pp. 111-22, 1991. https://doi.org/10.1016/0160-5402(91)90002-M
  11. R. F. Thompson and J. J. Kim, "Memory systems in the brain and localization of a memory", Proc Natl Acad Sci U S A, vol. 93, pp. 13438-44, 1996. https://doi.org/10.1073/pnas.93.24.13438
  12. I. Tanida, E. Tanida-Miyake, M. Komatsu, T. Ueno, and E. Kominami, "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p", J Biol Chem, vol. 277, pp. 13739-13744, 2002. https://doi.org/10.1074/jbc.M200385200
  13. R. Morris, "Developments of a water-maze procedure for studying spatial learning in the rat", J Neurosci Methods, vol. 11, pp. 47-60, 1984. https://doi.org/10.1016/0165-0270(84)90007-4