The Korean journal of internal medicine

The Korean Association of Internal Medicine (대한내과학회)
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Clinical significance of nuclear factor ${\kappa}B$ and chemokine receptor CXCR4 expression in patients with diffuse large B-cell lymphoma who received rituximab-based therapy

  • Shin, Ho Cheol (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Seo, Jongwon (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Kang, Byung Woog (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Moon, Joon Ho (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Chae, Yee Soo (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Lee, Soo Jung (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Lee, Yoo Jin (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Han, Seoae (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Seo, Sang Kyung (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Kim, Jong Gwang (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Sohn, Sang Kyun (Department of Hematology and Oncology, Kyungpook National University Hospital) ;
  • Park, Tae-In (Department of Pathology, Kyungpook National University Hospital)
  • Received : 2013.05.05
  • Accepted : 2014.01.06
  • Published : 2014.11.01
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Abstract

Background/Aims: This study investigated the expression of nuclear factor ${\kappa}B$ (NF-${\kappa}B$) and the chemokine receptor (CXCR4) in patients with diffuse large B-cell lymphoma (DLBCL) who received rituximab-based therapy. Methods: Seventy patients with DLBCL and treated with rituximab-CHOP (R-CHOP) were included, and immunohistochemistry was performed to determine the expression of NF-${\kappa}B$ ($I{\kappa}B$ kinase ${\alpha}$, p50, and p100/p52) and CXCR4. To classify DLBCL cases as germinal center B-cell-like (GCB) and non-GCB, additional immunohistochemical expression of CD10, bcl-6, or MUM1 was used in this study. The expression was divided into two groups according to the intensity score (negative, 0 or 1+; positive, 2+ or 3+). Results: The median age of the patients was 66 years (range, 17 to 87), and 58.6% were male. Twenty-seven patients (38.6%) had stage III or IV disease at diagnosis. Twenty-three patients (32.9%) were categorized as high or high-intermediate risk according to their International Prognostic Indexs (IPIs). The overall incidence of bone marrow involvement was 5.7%. Rates of positive NF-${\kappa}B$ and CXCR4 expression were 84.2% and 88.6%, respectively. High NF-${\kappa}B$ expression was associated with CXCR4 expression (p = 0.002), and 56 patients (80.0%) showed coexpression. However, the expression of NF-${\kappa}B$ or CXCR4 was not associated with overall survival and EFS. On multivariate analysis that included age, gender, performance status, stage, and the IPI, no significant association between the grade of NF-${\kappa}B$ or CXCR4 expression and survival was observed. Conclusions: The current study suggests that the tissue expression of NF-${\kappa}B$ and CXCR4 may not be an independent prognostic marker in DLBCL patients treated with R-CHOP.

Keywords

Acknowledgement

Supported by : Kyungpook National University

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