The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of the Large-Scale Oligonucleotide Chip for the Diagnosis of Plant Viruses and its Practical Use

  • Nam, Moon (Institute of Plant Medicine, Kyungpook National University) ;
  • Kim, Jeong-Seon (Agricultural Microbiology Division, National Academy of Agricultural Science, RDA) ;
  • Lim, Seungmo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Chung Youl (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Jeong-Gyu (Department of Applied Biology, Chungnam National University) ;
  • Choi, Hong-Soo (Crop Protection Division, National Academy of Agricultural Science, RDA) ;
  • Lim, Hyoun-Sub (Department of Applied Biology, Chungnam National University) ;
  • Moon, Jae Sun (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Su-Heon (Institute of Plant Medicine, Kyungpook National University)
  • Received : 2013.08.30
  • Accepted : 2013.09.30
  • Published : 2014.03.01
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Abstract

A large-scale oligonucleotide (LSON) chip was developed for the detection of the plant viruses with known genetic information. The LSON chip contains two sets of 3,978 probes for 538 species of targets including plant viruses, satellite RNAs and viroids. A hundred forty thousand probes, consisting of isolate-, species- and genus-specific probes respectively, are designed from 20,000 of independent nucleotide sequence of plant viruses. Based on the economic importance, the amount of genome information, and the number of strains and/or isolates, one to fifty-one probes for each target virus are selected and spotted on the chip. The standard and field samples for the analysis of the LSON chip have been prepared and tested by RT-PCR. The probe's specific and/or nonspecific reaction patterns by LSON chip allow us to diagnose the unidentified viruses. Thus, the LSON chip in this study could be highly useful for the detection of unexpected plant viruses, the monitoring of emerging viruses and the fluctuation of the population of major viruses in each plant.

Keywords

References

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