The Journal of Advanced Prosthodontics

The Korean Academy of Prosthodonitics (대한치과보철학회)
권호 표지
DOI QR코드

DOI QR Code

Investigation of anodized titanium implants coated with triterpenoids extracted from black cohosh: an animal study

  • Park, In-Phill (Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Kang, Tae-Joo (Department of Orthodontics, School of Dentistry, Seoul National University) ;
  • Heo, Seong-Joo (Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Koak, Jai-Young (Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University) ;
  • Kim, Ju-Han (Department of Oriental Medicinal Materials & Processing, College of Life Sciences, KyungHee University) ;
  • Lee, Joo-Hee (Department of Prosthodontics, Asan Medical Center, College of Medicine, University of Ulsan) ;
  • Lee, Shin-Jae (Department of Orthodontics, School of Dentistry, Seoul National University) ;
  • Kim, Seong-Kyun (Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University)
  • Received : 2012.12.27
  • Accepted : 2013.12.25
  • Published : 2014.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

PURPOSE. The purpose of this study was to evaluate bone response to anodized titanium implants coated with the extract of black cohosh, Asarum Sieboldii, and pharbitis semen. MATERIALS AND METHODS. Forty anodized titanium implants were prepared as follows: group 1 was for control; group 2 were implants soaked in a solution containing triterpenoids extracted from black cohosh for 24 hours; group 3 were implants soaked in a solution containing extracts of black cohosh and Asarum Sieboldii for 24 hours; group 4 were implants soaked in a solution containing extracts of pharbitis semen for 24 hours. The implants from these groups were randomly and surgically implanted into the tibiae of ten rabbits. After 1, 2, and 4 weeks of healing, the nondecalcified ground sections were subjected to histological observation, and the percentage of bone-to-implant contact (BIC%) was calculated. RESULTS. All groups exhibited good bone healing with the bone tissue in direct contact with the surface of the implant. Group 2 ($52.44{\pm}10.98$, $25.54{\pm}5.56$) showed a significantly greater BIC% compared to that of group 3 ($45.34{\pm}5.00$, $22.24{\pm}2.20$) with respect to the four consecutive threads and total length, respectively. The BIC% of group 1 ($25.22{\pm}6.00$) was significantly greater than that of group 3 ($22.24{\pm}2.20$) only for total length. CONCLUSION. This study did not show any remarkable effects of the extract of black coshosh and the other natural products on osseointegration of anodized titanium implants as coating agents. Further studies about the application method of the natural products on to the surface of implants are required.

Keywords

References

  1. Brånemark PI, Adell R, Breine U, Hansson BO, Lindström J, Ohlsson A. Intra-osseous anchorage of dental prostheses. I. Experimental studies. Scand J Plast Reconstr Surg 1969;3:81-100. https://doi.org/10.3109/02844316909036699
  2. Ostman PO. Immediate/early loading of dental implants. Clinical documentation and presentation of a treatment concept. Periodontol 2000 2008;47:90-112. https://doi.org/10.1111/j.1600-0757.2007.00244.x
  3. Albrektsson T, Wennerberg A. Oral implant surfaces: Part 1- review focusing on topographic and chemical properties of different surfaces and in vivo responses to them. Int JProsthodont 2004;17:536-43.
  4. Albrektsson T, Wennerberg A. Oral implant surfaces: Part 2- review focusing on clinical knowledge of different surfaces. Int J Prosthodont 2004;17:544-64.
  5. Junker R, Dimakis A, Thoneick M, Jansen JA. Effects of implant surface coatings and composition on bone integration: a systematic review. Clin Oral Implants Res 2009;20:185-206. https://doi.org/10.1111/j.1600-0501.2009.01777.x
  6. Avila G, Misch K, Galindo-Moreno P, Wang HL. Implant surface treatment using biomimetic agents. Implant Dent 2009;18:17-26. https://doi.org/10.1097/ID.0b013e318192cb7d
  7. Gullo VP, McAlpine J, Lam KS, Baker D, Petersen F. Drug discovery from natural products. J Ind Microbiol Biotechnol 2006;33:523-31. https://doi.org/10.1007/s10295-006-0107-2
  8. Lee KH. Discovery and development of natural product-derived chemotherapeutic agents based on a medicinal chemistry approach. J Nat Prod 2010;73:500-16. https://doi.org/10.1021/np900821e
  9. Putnam SE, Scutt AM, Bicknell K, Priestley CM, Williamson EM. Natural products as alternative treatments for metabolic bone disorders and for maintenance of bone health. Phytother Res 2007;21:99-112. https://doi.org/10.1002/ptr.2030
  10. Li JX, Yu ZY. Cimicifugae rhizoma: from origins, bioactive constituents to clinical outcomes. Curr Med Chem 2006;13:2927-51. https://doi.org/10.2174/092986706778521869
  11. Kim HY, Kim CS, Jhon GJ, Moon IS, Choi SH, Cho KS, Chai JK, Kim CK. The effect of safflower seed extract on periodontal healing of 1-wall intrabony defects in beagle dogs. J Periodontol 2002;73:1457-66. https://doi.org/10.1902/jop.2002.73.12.1457
  12. Baek EJ. The extract of Pharbitis nil Choisy has bone regenerative potential in the healing process of calvarial defects in a rabbit model. Master's Thesis, Kyunghee Univ, Seoul, 2009.
  13. Chen LL, Tang Q, Yan J. Therapeutic effect of aqueous-extract from a traditional Chinese medical herb Drynaria fortunei on rat experimental model of alveolar bone resorption. Zhongguo Zhong Yao Za Zhi 2004;29:549-53.
  14. Kesavalu L, Vasudevan B, Raghu B, Browning E, Dawson D, Novak JM, Correll MC, Steffen MJ, Bhattacharya A, Fernandes G, Ebersole JL. Omega-3 fatty acid effect on alveolar bone loss in rats. J Dent Res 2006;85:648-52. https://doi.org/10.1177/154405910608500713
  15. Wuttke W, Gorkow C, Seidlová-Wuttke D. Effects of black cohosh (Cimicifuga racemosa) on bone turnover, vaginal mucosa, and various blood parameters in postmenopausal women: a double-blind, placebo-controlled, and conjugated estrogens- controlled study. Menopause 2006;13:185-96. https://doi.org/10.1097/01.gme.0000174470.44822.57
  16. Li JX, Liu J, He CC, Yu ZY, Du Y, Kadota S, Seto H. Triterpenoids from Cimicifugae rhizoma, a novel class of inhibitors on bone resorption and ovariectomy-induced bone loss. Maturitas 2007;58:59-69. https://doi.org/10.1016/j.maturitas.2007.06.001
  17. Ernst M, Heath JK, Schmid C, Froesch RE, Rodan GA. Evidence for a direct effect of estrogen on bone cells in vitro. J Steroid Biochem 1989;34:279-84. https://doi.org/10.1016/0022-4731(89)90092-7
  18. Jung WS, Yoo HM, Seo SW, Cho JK, Son JW, Park MC, Choi CM, Yeon SR, Hwang SW, Kim YW, Song DS, Chae YS, Park SJ, Shin MK, Song HJ. Anti-inflammatory effect of extract of Asaum sieboldii in LPS-stimulated Murine peritoneal macrophage. Korean J Hebol 2006;21:189-95.
  19. Riancho JA, Zarrabeitia MT, Fernandez-Luna JL, Gonzalez- Macias J. Mechanisms controlling nitric oxide synthesis in osteoblasts Mol Cell Endocrinol 1995;107:87-92. https://doi.org/10.1016/0303-7207(94)03428-V
  20. Hashimoto K, Yanagisawa T, Okui Y, Ikeya Y, Maruno M, Fujita T. Studies on anti-allergic components in the roots of Asiasarum sieboldi. Planta Med 1994;60:124-7. https://doi.org/10.1055/s-2006-959432
  21. Jung da Y, Ha H, Lee HY, Kim C, Lee JH, Bae K, Kim JS, Kang SS. Triterpenoid saponins from the seeds of Pharbitis nil. Chem Pharm Bull (Tokyo). 2008;56:203-6. https://doi.org/10.1248/cpb.56.203
  22. Villegas M, Vargas D, Msonthi JD, Marston A, Hostettmann K. Isolation of the antifungal compounds falcarindiol and sarisan from Heteromorpha trifoliata. Planta Med 1988;54:36-7. https://doi.org/10.1055/s-2006-962326
  23. Zhao L, Chu PK, Zhang Y, Wu Z. Antibacterial coatings on titanium implants. J Biomed Mater Res B Appl Biomater 2009;91:470-80.
  24. Alt V, Bitschnau A, Osterling J, Sewing A, Meyer C, Kraus R, Meissner SA, Wenisch S, Domann E, Schnettler R. The effects of combined gentamicin-hydroxyapatite coating for cementless joint prostheses on the reduction of infection rates in a rabbit infection prophylaxis model. Biomaterials 2006;27:4627-34. https://doi.org/10.1016/j.biomaterials.2006.04.035
  25. Campbell AA, Song L, Li XS, Nelson BJ, Bottoni C, Brooks DE, DeJong ES. Development, characterization, and anti-microbial efficacy of hydroxyapatite-chlorhexidine coatings produced by surface-induced mineralization. J Biomed Mater Res 2000;53:400-7. https://doi.org/10.1002/1097-4636(2000)53:4<400::AID-JBM14>3.0.CO;2-Z
  26. Chen W, Liu Y, Courtney HS, Bettenga M, Agrawal CM, Bumgardner JD, Ong JL. In vitro anti-bacterial and biological properties of magnetron co-sputtered silver-containing hydroxyapatite coating. Biomaterials 2006;27:5512-7. https://doi.org/10.1016/j.biomaterials.2006.07.003
  27. Kim KH, Choi SU, Lee KR. Diterpene glycosides from the seeds of Pharbitis nil. J Nat Prod 2009;72:1121-7. https://doi.org/10.1021/np900101t
  28. Lee TH, Park YD, Lee KJ, Jung JH, Kang DH. Pharmaceutical composition for preventing or treating periodontal disease comprising extracts of Pharbitidis Semen 2008; Patent #1020080035877 from Korean Intellectual Property Office.
  29. Li LH, Kong YM, Kim HW, Kim YW, Kim HE, Heo SJ, Koak JY. Improved biological performance of Ti implants due to surface modification by micro-arc oxidation. Biomaterials 2004;25:2867-75. https://doi.org/10.1016/j.biomaterials.2003.09.048
  30. Choi JW, Heo SJ, Koak JY, Kim SK, Lim YJ, Kim SH, Lee JB. Biological responses of anodized titanium implants under different current voltages. J Oral Rehabil 2006;33:889-97. https://doi.org/10.1111/j.1365-2842.2006.01669.x
  31. Park JM, Koak JY, Jang JH, Han CH, Kim SK, Heo SJ. Osseointegration of anodized titanium implants coated with fibroblast growth factor-fibronectin (FGF-FN) fusion protein. Int J Oral Maxillofac Implants 2006;21:859-66.
  32. Lan J, Wang ZF, Shi B, Xia HB, Cheng XR. The influence of recombinant human BMP-2 on bone-implant osseointegration: biomechanical testing and histomorphometric analysis. Int J Oral Maxillofac Surg 2007;36:345-9. https://doi.org/10.1016/j.ijom.2006.10.019
  33. Lee JE, Heo SJ, Koak JY, Kim SK, Han CH, Lee SJ. Healing response of cortical and cancellous bone around titanium implants. Int J Oral Maxillofac Implants 2009;24:655-62.
  34. Yang GL, He FM, Yang XF, Wang XX, Zhao SF. Bone responses to titanium implants surface-roughened by sandblasted and double etched treatments in a rabbit model. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;106:516-24. https://doi.org/10.1016/j.tripleo.2008.03.017
  35. Burgos PM, Rasmusson L, Meirelles L, Sennerby L. Early bone tissue responses to turned and oxidized implants in the rabbit tibia. Clin Implant Dent Relat Res 2008;10:181-90. https://doi.org/10.1111/j.1708-8208.2007.00074.x
  36. Donath K, Breuner G. A method for the study of undecalcified bones and teeth with attached soft tissues. The Säge- Schliff (sawing and grinding) technique. J Oral Pathol 1982;11:318-26. https://doi.org/10.1111/j.1600-0714.1982.tb00172.x
  37. Wennerberg A, Albrektsson T, Andersson B. Bone tissue response to commercially pure titanium implants blasted with fine and coarse particles of aluminum oxide. Int J Oral Maxillofac Implants 1996;11:38-45.
  38. Palombo EA. Traditional Medicinal Plant Extracts and Natural Products with Activity against Oral Bacteria: Potential Application in the Prevention and Treatment of Oral Diseases. Evid Based Complement Alternat Med 2011;2011:680354.
  39. Lee SY, Koak JY, Heo SJ, Kim SK, Lee SJ, Nam SY. Osseointegration of anodized titanium implants coated with poly(lactide-co-glycolide)/basic fibroblast growth factor by electrospray. Int J Oral Maxillofac Implants 2010;25:315-20.
  40. Stenport VF, Johansson C, Heo SJ, Aspenberg P, Albrektsson T. Titanium implants and BMP-7 in bone: an experimental model in the rabbit. J Mater Sci Mater Med 2003;14:247-54.
  41. Roberts WE, Smith RK, Zilberman Y, Mozsary PG, Smith RS. Osseous adaptation to continuous loading of rigid endosseous implants. Am J Orthod 1984;86:95-111. https://doi.org/10.1016/0002-9416(84)90301-4