Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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The Effects of Several Halophytes on Insulin Resistance in Otsuka Long-evans Tokushima Fatty Rats

OLETF 쥐에서 칠면초와 세발나물의 인슐린 저항성 개선 효과

  • Cho, Jeong-Yong (Department of Food Science & Biotechnology and Solar Salt Research Center, Mokpo National University) ;
  • Huang, Zhangjun (Department of Food Science & Biotechnology and Solar Salt Research Center, Mokpo National University) ;
  • Park, Sun-Young (Department of Food Science & Biotechnology and Solar Salt Research Center, Mokpo National University) ;
  • Park, Kyung-Hee (Department of Food Science & Biotechnology and Solar Salt Research Center, Mokpo National University) ;
  • Pai, Tong-Kun (Department of Food and Nutrition, Anyang University) ;
  • Kim, So-Young (Division of Functional Food & Nutrition, National Academy of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Kim, Haeng-Ran (Division of Functional Food & Nutrition, National Academy of Agricultural Sciences, Rural Development Administration (RDA)) ;
  • Ham, Kyung-Sik (Department of Food Science & Biotechnology and Solar Salt Research Center, Mokpo National University)
  • 조정용 (목포대학교 식품공학과 및 천일염연구센터) ;
  • ;
  • 박선영 (목포대학교 식품공학과 및 천일염연구센터) ;
  • 박경희 (목포대학교 식품공학과 및 천일염연구센터) ;
  • 배동근 (안양대학교 식품영양학과) ;
  • 김소영 (농촌진흥청 국립농업과학원) ;
  • 김행란 (농촌진흥청 국립농업과학원) ;
  • 함경식 (목포대학교 식품공학과 및 천일염연구센터)
  • Received : 2013.09.17
  • Accepted : 2013.12.02
  • Published : 2014.02.28
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Abstract

We evaluated preventive effects of Suaeda japonica (SJ) and Spergularia marina Griseb (SMG) on the insulin resistance in Otsuka Long-Evans Tokushima Fatty (OLETF) rats. The 10-week old OLETF rats were fed diets containing 3% (w/w) SJ and SMG for 18 weeks. Fasting blood glucose levels in SJ and SMG groups, measured using the oral glucose tolerance test, were lower than that of the control rats. The SMG group showed significantly lower levels of insulin, glycated hemoglobin, triglyceride, and total cholesterol than the control group. In addition, these levels were relatively lower in the SJ group than those in the control rats. The SJ and SMG groups had relatively lower protein levels of nuclear factor-kappa B (NF-${\kappa}B$) p65 in adipose tissue and serine phosphorylated insulin receptor substrate 1 (IRS-1) in skeletal muscle than the control group. These results suggest that SJ and SMG prevent insulin resistance and SMG in particular reduces blood triglyceride and total cholesterol levels.

본 연구에서는 염생식물인 칠면초와 세발나물의 인슐린 저항성을 개선하는 효과를 조사하였다. 본 실험에 사용한 OLETF쥐는 CCK-1 수용체의 결함으로 인해 인슐린 저항성을 걸쳐 제 2형 당뇨병이 유발되는데, 인슐린 저항성이 진행되는 10주령부터 28주령이 될 때까지 칠면초와 세발나물을 18주 동안 섭취시켰다. 염생식물식이군들의 체중이나 공복혈당 변화는 식이기간 동안 전반적으로 대조군과 유의적인 차이를 보이지 않았다. Oral glucose tolerance test에서 염생식물 섭취가 당 내성을 개선하는 효과를 보였으나 그 혈당 변화를 면적으로 환산하였을 때 각 식이군들 간의 유의적인 차이는 관찰되지 않았다. 혈중 insulin 및 HbA1c 수치는 유의하게 낮은 수치를 보였으며, adiponectin 수치는 높고 leptin과 ghrelin 수치는 낮았으나 현저한 차이는 관찰되지 않았다. 세발나물식이군의 혈중 중성지질과 총 cholesterol 수치는 대조군에 비해 유의적으로 더 낮았다. 혈중 지질산화물 함량은 각 식이군 간 유의차가 관찰되지 않았으나 염생식물식이군에서 더 낮은 경향을 보였으며, NF-${\kappa}B$ p65는 지방조직에서 유의적으로 낮은 발현량을 보였다. 또한 두 염생식물식이군에서 인슐린 신호전달의 negative regulator인 $pIRS1^{Ser307}$의 발현량은 대조군에 비해 더 낮음을 확인하였다. 그러므로 칠면초와 세발나물은 생후 성장하면서 인슐린 저항성이 생기는 OLETF쥐의 인슐린 저항성을 현저하게 줄여주지는 못하였으나 그 개선 효과는 있는 것으로 시사되며, 두 염생식물의 급여 기간을 늘려 제 2형 당뇨병을 예방하는 효과에 대한 섬세한 검토가 보완되어야 할 것으로 사료된다.

Keywords

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