CELLMED (셀메드)

Cellmed Orthocellular Medicine and Pharmaceutical Association (셀메드 세포교정의약학회)
권호 표지
DOI QR코드

DOI QR Code

Evaluation of anti-inflammatory effect of traditional medicinal plants, Gynura segetum

  • Seow, Lay-Jing (School of Pharmaceutical Sciences, Universiti Sains Malaysia) ;
  • Beh, Hooi-Kheng (School of Pharmaceutical Sciences, Universiti Sains Malaysia) ;
  • Sadikun, Amirin (School of Pharmaceutical Sciences, Universiti Sains Malaysia) ;
  • Asmawi, Mohd Zaini (School of Pharmaceutical Sciences, Universiti Sains Malaysia)
  • Received : 2013.12.18
  • Accepted : 2014.02.07
  • Published : 2014.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Gynura segetum, family Asteraceae is a cultivated species and can be found growing in the tropical regions of Indonesia and Malaysia. The plant is known for its use for the treatment of cancer, inflammation, diabetes, hypertension and skin afflictions. In the current study, anti-inflammatory effect of Gynura segetum leaf has been investigated. The present study investigated the in vivo anti-inflammatory effect of Gynura segetum leaf by using hen's egg test-chorioallantoic membrane (HET-CAM) assay. Different solvent extract of Gynura segetum leaf were tested, the most active methanol extract was further fractionated and tested. Among the extracts tested, the methanol extract showed a significant good anti-inflammatory effect (76.8% inhibition at 50 ${\mu}g/disc$) on the HET-CAM assay as compared with the anti-inflammatory drug indomethacin (82.1% inhibition). However, the fractionated sample exhibited a significantly lower activity in comparison to crude methanol extract. The results demonstrated that Gynura segetum leaf displayed remarkable anti-inflammatory effects, which support the folkloric uses of this plant for treatment of inflammation.

Keywords

References

  1. Abdillahi HS, Finnie JF, Van Staden J. Anti-inflammatory, antioxidant, anti-tyrosinase and phenolic contents of four Podocarpus species used in traditional medicine in South Africa. J Ethnopharmacol. 2011;136:496-503. https://doi.org/10.1016/j.jep.2010.07.019
  2. Ahn EK, Jeon HJ, Lim EJ, Jung HJ, Park EH. Anti-inflammatory and anti-angiogenic activities of Gastrodia elata Blume. J Ethnopharmacol. 2007;110:476-482. https://doi.org/10.1016/j.jep.2006.10.006
  3. Burgermeister J, Paper DH, Vogl H, Linhardt RJ, Franza G. LaPSvS1, a ($1{\rightarrow}3)-{\beta}$- galactan sulfate and its effect on angiogenesis in vivo and in vitro. Carbohyd Res. 2002;337:1459-1466. https://doi.org/10.1016/S0008-6215(02)00163-5
  4. Cho SY, Lee YN, Park HJ. Optimization of ethanol extraction and further purification of isoflavones from soybean sprout cotyledon. Food Chem. 2009;117:312-317. https://doi.org/10.1016/j.foodchem.2009.04.003
  5. Doss A, Phil M. Preliminary phytochemical screening of some Indian medicinal plants. Anc Sci Life. 2009;29:12-16.
  6. Griffioen AW, Molema G. Angiogenesis: potentials for pharmacologic intervention in the treatment of cancer, cardiovascular diseases, and chronic inflammation. Pharmacol Rev. 2000;52:237-268.
  7. Jackson JR, Seed MP, Kircher CH, Willoughby DA, Winkler JD. The codependemce of angiogenesis and chronic inflammation. FASEB J. 1997;11:457-465. https://doi.org/10.1096/fasebj.11.6.9194526
  8. Jung HJ, Song YS, Lim CJ, Park EH. Anti-inflammatory, anti-angiogenic and anti-nociceptive activities of an ethanol extract of Salvia plebeia R. Brown. J Ethnopharmacol. 2009;126:355-360. https://doi.org/10.1016/j.jep.2009.08.031
  9. Kim SH, Song YS, Kim SK, Kim BC, Lim CJ, Park EH. Anti-inflammatory and related pharmacological activities of the n-BuOH subfraction of mushroom Phellinus linteus. J Ethnopharmacol. 2004;93:141-146. https://doi.org/10.1016/j.jep.2004.03.048
  10. Krenn L, Paper DH. Inhibition of angiogenesis and inflammation by an extract of red clover (Trifolim pratense L.). Phytomedicine. 2009;16:1083-1088. https://doi.org/10.1016/j.phymed.2009.05.017
  11. Masresha B, Makonnen E, Debella A. In vivo anti-inflammatory activities of Ocimum suave in mice. J Ethnopharmacol. 2012;142: 201-205. https://doi.org/10.1016/j.jep.2012.04.041
  12. Mathur R, Gupta SK, Singh N, Mathur S, Kochupillai V, Velpandian T. Evaluation of the effect of Withania somnifera root extracts on cell cycle and angiogenesis. J Ethnopharmacol. 2006;105:336-341. https://doi.org/10.1016/j.jep.2005.11.020
  13. Min JK, Han KY, Kim EC, Kim YM, Lee SW, Kim OH. Capsaicin inhibits in vitro and in vivo angiogenesis. Cancer Res. 2004;64:644-651. https://doi.org/10.1158/0008-5472.CAN-03-3250
  14. Peng CC, Hsieh CL, Lin LY, Chyau CC, Cheng YT, Chen KC, Peng RY. Anisotropic diffusion deviates chicken embryo chorioallantoic membrane assay (CAM) to reflect inherent therapeutic behaviors. Colloids Surf B Biointerfaces. 2009;70:29-34. https://doi.org/10.1016/j.colsurfb.2008.12.008
  15. Rathore SK, Bhatt S, Dhyani S, Jain A. Preliminary phytochemical screening of medicinal plant Ziziphus mauritiana Lam. Fruits. Int J Curr Pharm Res. 2012;4:160-162.
  16. Seow LJ, Beh HK, Abdul Majid AMS, Murugaiyah V, Ismail N, Asmawi MZ. Anti-angiogenic activity of Gynura segetum leaf extracts and its fractions. J Ethnopharmacol. 2011;134:221-227. https://doi.org/10.1016/j.jep.2010.12.007
  17. Seow LJ, Beh HK, Sadikun A, Asmawi MZ. Preliminary Phytochemical and Physicochemical Characterization of Gynura segetum (Lour.) Merr (Compositae) Leaf. Trop J Pharm Res. 2013;12:777-782.
  18. Torey A, Sasidharan S, Yeng C, Yoga Latha LY. Standardization of Cassia spectabilis with respect to authenticity, assay and chemical constituent analysis. Molecules. 2010;15:3411-3420. https://doi.org/10.3390/molecules15053411
  19. Walsh DA, Pearson CI. Angiogenesis in the pathogenesis of inflammatory joint and lung diseases. Arthritis Res. 2001;3:147-153. https://doi.org/10.1186/ar292
  20. Zhu BR, Pu SB, Wang KDG, Xu DR, Zhou HH. Chemical constituents of the aerial part of Gynura segetum. Biochem Syst Ecol. 2013;46:4-6. https://doi.org/10.1016/j.bse.2012.08.003

Cited by

  1. Anti-inflammatory and antioxidant activities of the methanol extract of Gynura segetum leaf vol.23, pp.1, 2014, https://doi.org/10.1016/j.intimp.2014.08.020