Journal of the Optical Society of Korea

Optical Society of Korea (한국광학회)
권호 표지
DOI QR코드

DOI QR Code

Multimodal Imaging of Sarcopenia using Optical Coherence Tomography and Ultrasound in Rat Model

  • Jeon, Byeong Hwan (School of Sports and Health, Kyungsung University) ;
  • Chae, Yu-Gyeong (Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology, Pukyong National University) ;
  • Hwang, Sang Seok (Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology, Pukyong National University) ;
  • Kim, Dong Kyu (Innovative Biomedical Technology Research Center, College of Medicine, Kosin University) ;
  • Oak, Chulho (Innovative Biomedical Technology Research Center, College of Medicine, Kosin University) ;
  • Park, Eun-Kee (Innovative Biomedical Technology Research Center, College of Medicine, Kosin University) ;
  • Ahn, Yeh-Chan (Department of Biomedical Engineering and Center for Marine-Integrated Biomedical Technology, Pukyong National University)
  • Received : 2014.01.23
  • Accepted : 2014.01.31
  • Published : 2014.02.25
Download PDF
⟨ Previous Next ⟩

Abstract

Sarcopenia, or reduced muscle mass and volume, is due to various factors such as senile change, neuronal degeneration, drug, malignancy, and sepsis. Sarcopenia with the aging process has been evidenced by the decline in muscle mass by 0.5 to 1% per year with 3-5% reduction in muscle strength for 10 years between the ages of 40 and 50, and a 1-2% of decline of mass every year in people aged 60-70. Therefore, early diagnosis and understanding the mechanism of sarcopenia are crucial in the prevention of muscle loss. However, it is still difficult to image changes of muscle microstructure due to a lack of techniques. In this study, we developed an animal model using denervated rats to induce a rapid atrophy in the tibialis anterior (TA) and imaged its structural changes using optical coherence tomography (OCT) along with histologic and ultrasound analyses. Ultrasound showed changes of overall muscle size. Histology revealed that the atrophic TA muscle displayed an increased size variability of muscle fiber and inflammatory changes. Three dimensional OCT imaged the changes of perimysial grid and muscle fiber structure in real time without sacrifice. These observed advantages of multimodal imaging using OCT and ultrasound would provide clinical benefits in the diagnosis of sarcopenia.

Keywords

References

  1. A. E. Emery, "The muscular dystrophies," Lancet 359, 687-695 (2002). https://doi.org/10.1016/S0140-6736(02)07815-7
  2. S. B. Charge and M. A. Rudnicki, "Cellular and molecular regulation of muscle regeneration," Physiol. Rev. 84, 209-238 (2004). https://doi.org/10.1152/physrev.00019.2003
  3. W. J. Evans, "Skeletal muscle loss: Cachexia, sarcopenia, and inactivity," Am. J. Clin. Nutr. 91, 1123S-1127S (2010). https://doi.org/10.3945/ajcn.2010.28608A
  4. M. Sandri, "Signaling in muscle atrophy and hypertrophy," Physiology (Bethesda) 23, 160-170 (2008). https://doi.org/10.1152/physiol.00041.2007
  5. V. A. Hughes, W. R. Frontera, M. Wood, W. J. Evans, G. E. Dallal, R. Roubenoff, and M. A. F. Singh, "Longitudinal muscle strength changes in older adults: influence of muscle mass, physical activity, and health," J. Gerontol. A. Biol. Sci. Med. Sci. 56, B209-17 (2001). https://doi.org/10.1093/gerona/56.5.B209
  6. M. V. Narici and N. Maffulli, "Sarcopenia: Characteristics, mechanisms and functional significance," Br. Med. Bull. 95, 139-159 (2010). https://doi.org/10.1093/bmb/ldq008
  7. J. Ward, "Sarcopenia and sarcopenic obesity: Is it time the health system accepted fitness of older people as a health responsibility?," Australas. J. Ageing 30, 61-62 (2011). https://doi.org/10.1111/j.1741-6612.2011.00536.x
  8. W. R. Frontera, V. A. Hughes, R. A. Fielding, M. A. Fiatarone, W. J. Evans, and R. Roubenoff, "Aging of skeletal muscle: a 12-yr longitudinal study," J. Appl. Physiol. 88, 1321-1326 (2000).
  9. T. Rantanen, R. Sakari-Rantala, and E. Heikkinen, "Muscle strength before and mortality after a bone fracture in older people," Scand. J. Med. Sci. Sports 12, 296-300 (2002). https://doi.org/10.1034/j.1600-0838.2002.102100.x
  10. F. Dela and M. Kjaer, "Resistance training, insulin sensitivity and muscle function in the elderly," Essays Biochem. 42, 75-88 (2006). https://doi.org/10.1042/bse0420075
  11. B. R. Klyen, J. J. Armstrong, S. G. Adie, H. G. Radley, M. D. Grounds, and D. D. Sampson, "Three-dimensional optical coherence tomography of whole-muscle autografts as a precursor to morphological assessment of muscular dystrophy in mice," J. Biomed. Opt. 13, 011003 (2008). https://doi.org/10.1117/1.2870170
  12. B. R. Klyen, T. Shavlakadze, H. G. Radley-Crabb, M. D. Grounds, and D. D. Sampson, "Identification of muscle necrosis in the mdx mouse model of Duchenne muscular dystrophy using three-dimensional optical coherence tomography," J. Biomed. Opt. 16, 076013 (2011). https://doi.org/10.1117/1.3598842
  13. R. M. Lovering, S. B. Shah, S. J. P. Pratt, W. Gong, and Y. Chen, "Architecture of healthy and dystrophic muscles detected by optical coherence tomography," Muscle Nerve 47, 588-590 (2013). https://doi.org/10.1002/mus.23711
  14. X. Yang, D. Lorenser, R. A. McLaughlin, R. W. Kirk, M. Edmond, M. C. Simpson, M. D. Grounds, and D. D. Sampson, "Imaging deep skeletal muscle structure using a high-sensitivity ultrathin side-viewing optical coherence tomography needle probe," Biomed. Opt. Express 5, 136-148 (2014). https://doi.org/10.1364/BOE.5.000136
  15. J. P. Hyatt, R. R. Roy, K. M. Baldwin, and V. R. Edgerton, "Nerve activity-independent regulation of skeletal muscle atrophy: Role of MyoD and myogenin in satellite cells and myonuclei," Am. J. Physiol. Cell Physiol. 285, C1161-C1173 (2003). https://doi.org/10.1152/ajpcell.00128.2003
  16. J. M. Sacheck, J. P. Hyatt, A. Raffaello, R. T. Jagoe, R. R. Roy, V. R. Edgerton, S. H. Lecker, and A. L. Goldberg, "Rapid disuse and denervation atrophy involve transcriptional changes similar to those of muscle wasting during systemic diseases," FASEB J. 21, 140-155 (2007).
  17. D. Mayans, M. S. Cartwright, and F. O. Walker, "Neuromuscular ultrasonography: Quantifying muscle and nerve measurements," Phys. Med. Rehabil. Clin. N. Am. 23, 133-148 (2012). https://doi.org/10.1016/j.pmr.2011.11.009

Cited by

  1. Multiphoton imaging of myogenic differentiation in gelatin-based hydrogels as tissue engineering scaffolds vol.20, pp.1, 2016, https://doi.org/10.1186/s40824-016-0050-x