Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antioxidative Activity of Mushroom Water Extracts Fermented by Lactic Acid Bacteria

유산균 발효에 의한 버섯 추출물의 항산화 활성

  • 양희선 ((재)임실치즈과학연구소) ;
  • 최유진 ((재)임실치즈과학연구소) ;
  • 오현희 ((재)임실치즈과학연구소) ;
  • 문준성 ((재)임실치즈과학연구소) ;
  • 정후길 ((재)임실치즈과학연구소) ;
  • 김경제 ((재)장흥군버섯산업연구원) ;
  • 최봉석 ((재)장흥군버섯산업연구원) ;
  • 이중원 ((재)장흥군버섯산업연구원) ;
  • 허창기 ((재)임실치즈과학연구소)
  • Received : 2013.09.10
  • Accepted : 2013.10.28
  • Published : 2014.01.31
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Abstract

This study was focused on the development of fermented mushroom water extracts with antioxidative activities. Mushroom water extracts were fermented with Bifidobacterium bifidum, Lactobacillus plantarum, Lactobacillus acidophilus, Leuconostoc lactis, Streptococcus thermophilus and Lactobacillus sakei subsp. LI033 was isolated from kimchi. Fermented mushroom water extracts increased DPPH and ABTS radical scavenging activities in a dose-dependent manner. However, radical scavenging activity of fermented Phellinus linteus and Ganoderma lucidum water extracts was decreased compared to non-fermented mushroom water extracts. Antioxidative activity of fermented mushroom water extracts was also confirmed by xanthin oxidase (XO) inhibition and superoxide dismutase (SOD) activities at the same concentration. As the fermentation progressed, fermented mushroom water extracts increased XO inhibition activity and SOD activity. In conclusion, fermented mushroom water extracts were tentatively identified to enhance enzyme activity.

본 연구에서는 유산균을 이용하여 3종의 버섯(상황, 영지, 표고)을 단계적으로 발효하여 항산화 활성을 비교하였다. 항산화 활성이 밝혀진 표준 유산균 5종을 선발하여 버섯 열수 추출물의 1차 발효에, 임실 지역 김치로부터 분리한 유산균 1종을 최종 선발하여 2차 발효에 이용하였다. 버섯 추출물과 1차, 2차 발효물의 항산화 활성 평가를 위해 DPPH 및 ABTS 라디칼 소거 정도를 비교하였다. 버섯 추출물 및 발효물은 농도 의존적으로 라디칼을 소거하였지만 유산균 발효를 통하여 상황과 영지의 경우 라디칼 소거 활성이 감소하였다. 이는 발효 과정 중 폴리페놀 화합물과 비타민 C, E 등 천연 항산화 물질들의 산화에 기인한 결과라고 사료되었다. 그러나 발효 중 생산되는 대사산물 및 유효성분은 XO 효소의 활성을 저해시키고 SOD 효소의 활성을 증가시켰으며, 1차 발효보다 2차 발효에서 활성이 증가되었다. 특히 5 mg/mL 이상의 농도에서 영지 1차 발효물은 90% 이상 XO 효소의 활성을 저해하였으며, 상황 1차 발효물은 3배 이상의 SOD 효소 활성을 증가시켰다. 이는 유산균에 의한 버섯 발효물이 효소의 활성을 저해 혹은 활성화시켜 항산화에 기여하는 것으로 판단되었다.

Keywords

References

  1. Lee YM, Kang DG, Kim MG, Choi DH, Lee HS. 2004. Isolation of antioxidants from the seeds of Xanthium strumarium. Korean J Ori Physiol Pathol 18: 792-796.
  2. Moon YG, Choi GS, Lee LJ, Kim KY, Heo MS. 2007. Lactic acid bacterias growth, antioxidant activities and antimicrobial activity on fish pathogenic bacteria by native plant extracts, Jeju island. Korean J Microbiol Biotechnol 35:210-219.
  3. Ames BN, Shigenaga MK, Hagen TM. 1993. Oxidants antioxidants and the degenerative disease of aging. P Natl Acad Sci USA 90: 7915-7922. https://doi.org/10.1073/pnas.90.17.7915
  4. Rhyu DY, Kim MS, Min OJ, Kim DW. 2008. Antioxidative effects of Phellinus linteus extract. Korean J Plant Res 21:91-95.
  5. Xu XM, Jun JY, Jeong IH. 2007. A study on the antioxidant activity of Hae-Songi mushroom (Hypsizigus marmoreus) hot water extracts. J Korean Soc Food Sci Nutr 36: 1351-1357. https://doi.org/10.3746/jkfn.2007.36.11.1351
  6. Hwang JG, Yun JK, Han KH, Do EJ, Lee JS, Lee EJ, Kim JB, Kim MR. 2011. Anti-oxidation and anti-aging effect of mixed extract from Korean medicinal herbs. Kor J Herbology 26: 111-117.
  7. Kim HH, Kwon JH, Park KH, Kim MH, Oh MH, Choe KI, Park SH, Jin HY, Kim SS, Lee MW. 2012. Screening of antioxidative activities and antiinflammatory activities in local native plants. Kor J Pharmacogn 43: 85-93.
  8. Kang HW. 2012. Antioxidant and anti-inflammatory effect of extracts from Flammulina velutipes (Curtis) singer. J Korean Soc Food Sci Nutr 41: 1072-1078. https://doi.org/10.3746/jkfn.2012.41.8.1072
  9. Kim JO, Jung MJ, Choi HJ, Lee JT, Lim AK, Hong JH, Kim DI. 2008. Antioxidative and biological activity of hot water and ethanol extracts from Phellinus linteus. J Korean Soc Food Sci Nutr 37: 684-690. https://doi.org/10.3746/jkfn.2008.37.6.684
  10. Mau JL, Lin HC, Song SF. 2002. Antioxidant properties of several speciality mushroom. Food Res Int 35: 519-526. https://doi.org/10.1016/S0963-9969(01)00150-8
  11. Jun DH, Kim HY, Han SI, Kim YH, Kim SG, Lee JT. 2013. Studies on antioxidant effect of mushroom complex. J Life Sci 23: 377-382. https://doi.org/10.5352/JLS.2013.23.3.377
  12. Kim HJ, Bae JT, Lee JW, Hwangbo MH. 2005. Antioxidant activity and inhibitive effects on human leukemia cells of edible mushrooms extract. Korean J Food Preserv 12: 80-85.
  13. Ham SS, Oh SW, Kim YK, Shin KS, Chang HY, Chung GH. 2003. Antioxidant and genotoxic inhibition activity of ethanol extract from the Inonotus obliquus. J Korean Soc Food Sci Nutr 32: 1071-1075. https://doi.org/10.3746/jkfn.2003.32.7.1071
  14. Lee SH, Park HJ, Young CS, Jin JH. 2004. Supplementary effect of Lentinus edodes on serum and hepatic lipid levels in spontaneously hypertensive rat. J Korean Soc Food Sci Nutr 37: 509-514.
  15. Park HJ, Heo Y, Kim JB. 2011. Immunomodulating effect of edible mushroom in mice. J Life Sci 21: 515-520. https://doi.org/10.5352/JLS.2011.21.4.515
  16. Park MH, Oh KY, Lee BW. 1998. Anti-cancer activity of Lentinus edoeds and Pleurotus astreatus. Korean J Food Sci Technol 30: 702-708.
  17. Park KY. 2012. Increased health functionality of fermented foods. Food Industry and Nutrition 17: 1-8.
  18. Ann YG. 2011. Probiotic lactic acid bacteria. Korean J Food & Nutr 24: 817-832. https://doi.org/10.9799/ksfan.2011.24.4.817
  19. Bang JH, Shin HJ, Choi HJ, Kim DW, Ahn CS, Jeong YK, Joo WH. 2012. Probiotic potential of lactobacillus isolates. J Life Sci 22: 251-258. https://doi.org/10.5352/JLS.2012.22.2.251
  20. Kim GE. 2011. Chacteristics & applications of lactobacillus sp. from kimchi. KSBB J 26: 374-380. https://doi.org/10.7841/ksbbj.2011.26.5.374
  21. Jeon JM, Choi SK, Kim YJ, Jang SJ, Cheon JW, Lee HS. 2011. Antioxidant and antiaging effect of ginseng berry extract fermented by lactic acid bacteria. J Soc Cosmet Scientists Korea 37: 75-81.
  22. Jung HK, Kim EY, Yae HS, Choi SJ, Jung JY, Juhn SL. 2000. Cholesterol-lowering effect of lactic acid bacteria and fermented milks as probiotic functional foods. Food Industry and Nutrition 5: 29-35.
  23. Yeo MH, Kim DM, Kim YH, Kim JH, Baek H, Chung MJ. 2008. Antitumor activity of CBT-AK5 purified from Lactobacillus casei against sarcoma-180 infected ICR mice. Korean J Dairy Sci Technol 26: 23-30.
  24. Kim SJ. 2005. Physicochemical characteristics of yogurt prepared with lactic acid bacteria isolated from kimchi. Korean J Food Culture 20: 337-340.
  25. Lee Y, Chang HC. 2008. Isolation and characterization of kimchi lactic acid bacteria showing anti-Helicobacter pylori activity. Korean J Microbiol Biotechnol 36: 106-114.
  26. Kim SY, Shin KS, Lee H. 2004. Immunopotentiating activities of cellular components of Lactobacillus brevis FSB-1. J Korean Soc Food Sci Nutr 33: 1552-1559. https://doi.org/10.3746/jkfn.2004.33.9.1552
  27. Park SH, Kim YA, Lee DK, Lee SJ, Chung MJ, Kang BY, Kim KJ, Ha NJ. 2007. Antibacterial activity and macrophage activation of lactic acid bacteria. J Environ Toxicol 22: 287-297.
  28. Kim KH, Yun YS, Chun SY, Yook HS. 2012. Antioxidant and antibacterial activities of grape pomace fermented by various microorganisms. J Korean Soc Food Sci Nutr 41:1049-1056. https://doi.org/10.3746/jkfn.2012.41.8.1049
  29. Kim YS, Chio GH, Lee KH. 2011. Change of antioxidative components and activity of fermented tea during fermentation period. J Korean Soc Food Sci Nutr 40: 1073-1078. https://doi.org/10.3746/jkfn.2011.40.8.1073
  30. Auh MS, Kim YS, Ahn SJ, Ahu JB, Kim KY. 2012. Comparison of property changes of black jujube and Zizyphus jujube extracts during lactic acid fermentation. J Korean Soc Food Sci Nutr 41: 1346-1355. https://doi.org/10.3746/jkfn.2012.41.10.1346
  31. Lee YK, Kim MK, Lee YC, Rho JH, Ma JY, Cho CW. 2011. Characteristic changes of Galgeuntang fermented with lactic acid bacteria. Korean J Food Sci Technol 43: 655-658. https://doi.org/10.9721/KJFST.2011.43.5.655
  32. Yang MC, Jeong SW, Ma JY. 2011. Analysis of constituents in sipjundaebo-tang fermented by lactic acid bacteria. Korean J Microbiol Biotechnol 39: 350-356.

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