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교배실험을 통한 북방전복 (Haliotis discus hannai)의 패각색 변이에 대한 유전적 지배

Genetic control of shell color variation in the Haliotis discus hannai by mating experiments

  • 박철지 (국립수산과학원 육종연구센터) ;
  • 남원식 (국립수산과학원 육종연구센터) ;
  • 이명석 (국립수산과학원 육종연구센터) ;
  • 강지윤 (국립수산과학원 육종연구센터) ;
  • 김경길 (국립수산과학원 육종연구센터)
  • 투고 : 2014.11.24
  • 심사 : 2014.12.26
  • 발행 : 2014.12.31

초록

본 연구는 북방전복의 보라색패각색의 유전적 지배를 명확히 파악하는 것을 목적으로 하였다. 환경적 요인과 유전적 요인을 명확히 구분하기 위하여 모든 실험구는 같은 사료를 공급하여 실험 하였으며, 또한 동시에 생산된 4가계를 혼합사육한 후 microsatellite DNA를 이용하여 친자확인으로 가계를 분리하여 가계간의 패각색의 분리를 확인하였다. 그 결과, 교배형태가 ♀Gr ${\times}$ ♂Gr (GG type), ♀Pu ${\times}$ ♂Gr (PG type) 및 ♀Gr ${\times}$ ♂Pu (GP type)의 경우 모든 자식개체의 패각색은 녹색으로 나타난 반면, ♀Pu ${\times}$ ♂Pu (PP type)의 교배구의 모든 자식개체는 보라색패각형으로 나타났다. 따라서 패각색 형태는 녹색이 우성, 보라색이 열성형질로 가정할 경우 패각색의 표현형 분리의 설명이 가능하며, 보라색패각색의 유전지배는 1개의 유전자좌에 대하여 2개의 대립유전자에 의해 결정된다고 생각된다.

Purple-colored shell individuals were discovered among normal green-colored shell individuals in artificial seed of Pacific abalone, Haliotis discus hannai, reared on an ordinary type of diatom and artificial diet. In the present study, factorial mating experiments were designed to clarify the genetic control of the variant (purple type) and normal (green type) of shell color. The parental population of purple type and green type individuals were derived from a single family between a female and male of each type of coloration. The all mating families were reared in same tank for the same breed environment. The individual of 4 type families were distinguished by paternity test using microsatellite DNA. In factorial mating experiments, all individuals offspring of GG (green type female and green type male), GP (green type female and purple type male) and PG (purple type female and green type male) mating types appeared to green type. In only PP (purple type female and purple type male) mating type, all individuals offspring appeared to purple type. The results suggested that the purple shell color is controlled by recessive purple type allele and a dominant green type allele at a single locus.

키워드

참고문헌

  1. Adamkewicz, L. and Castagna, M. (1988) Genetics of shell color and pattern in the bay scallop Argopecten irradians. Journal of Heredity, 79: 14-17.
  2. Cole, T.J. (1975) Inheritance of juvenile shell colour of the oyster drill, Urosalpix cinerea. Nature, 257: 794-795. https://doi.org/10.1038/257794a0
  3. Cowie, R.H. (1990) Climatic selection on body colour in the land snail Theba pisana (Pulmonata:Helicidae). Heredity, 65: 123-126. https://doi.org/10.1038/hdy.1990.78
  4. Fujiwara, M. (1995) Inheritance of yellow coloration of the shell in the cockle Fulvia mutica. Nippon Suisan Gakkaishi, 61: 927-928. https://doi.org/10.2331/suisan.61.927
  5. Heath, D.J. (1975) Colour, sunlight and internal temperatures in the land-snail Cepaea nemoralis (L.). Oecologia, 19: 29-38. https://doi.org/10.1007/BF00377587
  6. Hoagland, K.E. (1977) A gastropod color polymorphism: one adaptive strategy of phenotypic variation. The Biological Bulletin, 152: 360-372. https://doi.org/10.2307/1540424
  7. Innes, D.j. and Haley, L.E. (1977) Inheritance of a shell color poly-morphism in the mussel. Journal of Heredity, 68: 203-204.
  8. Kobayashi, T., Kawahara, I., Hasekura, O., Kijima, A. (2004) Genetic control of bluish shell color variation in the Pacific abalone Haliotis discus hannai. Journal of Shellfish Research, 23: 1153-1156.
  9. Li, Q., Park, C., Kijima, A. (2002) Isolation and characterization of microsatellite loci in the Pacific abalone, Haliotis discus hannai, Journal of Shellfish Research, 21: 811-815.
  10. Liu, X., Wu, F., Zhao, H., Zhang, G., Guo, X. (2009) A novel shell color variant of the Pacific abalone Haliotis discus hannai ino subject to genetic control and dietary influence. Journal of Shellfish Research, 28: 419-424 https://doi.org/10.2983/035.028.0226
  11. Ogino, C. and Ohta, E. (1963) Studies on the nutrition of abalone-I. Feeding trials of abalone, Haliotis discus Reeve, with artificial diets. Bulletin of the Japanese Society for the Science of Fish, 29: 691-694. https://doi.org/10.2331/suisan.29.691
  12. Park, C.J., Li, Q., Kobayashi, T., Kijima, A. (2003) Characterization novel microsatellite DNA marker in the Pacific abalone, Haliotis discus hannai. Fish genetics and Breeding science, 33: 19-24.
  13. Precht, H. and Plett, A. (1979) The influence of environmental factors on animal. III. Salinity. Zoologischer Anzeiger, 202: 425-436.
  14. Raffaeli, D. (1982) Recent ecological research on some European species of Littorina. The Journal of Molluscan Studies, 48: 342-354.
  15. Sakai, S. (1962) Ecological studies on the abalone, Haliotis discus hannai Ino-I. Experimental studies on the food habit. Bulletin of the Japanese Society of Scientific Fisheries, 28: 766-779. https://doi.org/10.2331/suisan.28.766
  16. Sekino, M., Saido, T., Fujita, T., Kobayashi, T., Takami, H. (2005) Microsatellite DNA markers of Ezo abalone (Haliotis discus hannai): a preliminary assessment of natural populations sampled from heavily stocked areas. Aquaculture, 243: 33-47. https://doi.org/10.1016/j.aquaculture.2004.10.013
  17. Sokolova, I.M. and Berger, V.J. (2000) Physiological variation related to shell colour polymorphism in White Sea Littorina saxatilis. Journal of Experimental Marine Biology and Ecology, 245: 1-23. https://doi.org/10.1016/S0022-0981(99)00132-X