Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Genetic characteristics of the novel insect pathogenic Bacillus thuringiensis subsp. aizawai strain

새로운 곤충병원성 Bacillus thuringiensis subsp. aizawai 균주의 유전학적 특성

  • Seo, Mi Ja (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Youn, Young Nam (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yu, Yong Man (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Ki Su (Bio Matertial Deptment, CHAMEDITECH, CO.)
  • 서미자 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 윤영남 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물학과) ;
  • 김기수 ((주)차메디텍 생물소재팀)
  • Received : 2014.10.28
  • Accepted : 2014.12.15
  • Published : 2014.12.31
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Abstract

For identifying the plasmid DNA coding cry gene of Bacillus thuringiensis subsp. aizawai KB098 with high insecticidal activity against Spodoptera exigua, mutant isolates with no crystal protein were produced by $42^{\circ}C$ incubation condition and then mutant plasmid DNA band patterns were compared with those of KB098. KB098 isolates had 4 cry genes, cry1Aa, cry1Ab, cry1C, cry1D, and also had been found seven plasmid DNA. Though the SDS-PAGE experiment, it was confirmed that mutant didn't produce 130~145kDa protein band involved in bipyramidal shape crystal. Also, five mutant isolates had no cry genes coding plasmid DNA in PCR. In result of comparison the plasmid DNA of KB098 and 5 mutant isolates, only 1 plasmid DNA band was left out in mutant plasmid DNA pattern, so that the missing band was extracted from the gel. The missing(disappeared) plasmid DNA was the largest molecular size among the 7 plasmid DNA of KB098 and it was also confirmed this plasmid DNA had all 4 cry genes through PCR.

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References

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