Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Kelpak® on the promotion of in vitro rooting in transgenic rose plantlets

Kelpak® 침지 처리에 의한 형질전환 장미 기내 식물체 발근 촉진

  • Lee, Su Young (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kwon, O Hyeon (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Lee, Hye Jin (National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Won Hee (National Institute of Horticultural & Herbal Science, Rural Development Administration)
  • 이수영 (농촌진흥청 국립원예특작과학원 화훼과) ;
  • 권오현 (농촌진흥청 국립원예특작과학원 화훼과) ;
  • 이혜진 (농촌진흥청 국립원예특작과학원 화훼과) ;
  • 김원희 (농촌진흥청 국립원예특작과학원 화훼과)
  • Received : 2014.10.21
  • Accepted : 2014.11.01
  • Published : 2014.12.31
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Abstract

In order to promote in vitro rooting in SOD2-transgenic rose plantlets, which were not well rooted in a rooting medium (MS medium with NAA $0.03mg{\cdot}L^{-1}$), we dipped the plantlets into liquid $Kelpak^{(R)}$ before placing them in the rooting medium. After 4 weeks, $Kelpak^{(R)}$ significantly promoted in vitro rooting in the plantlets. Therefore, $Kelpak^{(R)}$ can be used successfully to aid in the in vitro rooting of rose plantlets with roots that are not well-generated.

도입 유전자의 영향으로 발근유도배지(NAA $0.03mg{\cdot}L^{-1}$ 첨가 MS배지)에서도 발근이 잘 되지 않는 SOD2유전자 도입 장미 형질전환체의 $Kelpak^{(R)}$ 침지에 의한 발근 촉진가능성을 조사하였다. $Kelpak^{(R)}$ 액에 침지 후 발근유도배지에 배양 4주 후 장미 SOD2형질전환체의 기내뿌리 유도 및 뿌리생장이 촉진되었다. 또한 대조식물체인 비형질전환식물체도 $Kelpak^{(R)}$ 침지 처리후 발근유도배지에 배양하는 것이 $Kelpak^{(R)}$ 처리 없이 발근유도배지에 배양하는 것보다 뿌리수도 증가했을 뿐 아니라 뿌리길이 생장도 증진된 것으로 볼 때 $Kelpak^{(R)}$이 장미 기내식물체의 발근 촉진에 효과적인 것은 분명하다.

Keywords

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