Journal of Korean Dental Science

Korean Academy of Dental Science (대한치의학회)
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Effect of Chitosan on Expression of Osteogenic Genes during the Healing of Rat Extraction Socket

  • Youn, Gap-Hee (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University) ;
  • Jung, Seunggon (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University) ;
  • Lee, Tae-Hoon (Department of Oral Biochemistry, Dental Science Research Institute, School of Dentistry, Chonnam National University) ;
  • Kook, Min-Suk (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University) ;
  • Park, Hong-Ju (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University) ;
  • Oh, Hee-Kyun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Chonnam National University)
  • Received : 2014.10.23
  • Accepted : 2014.12.09
  • Published : 2014.12.30
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Abstract

Purpose: This study was performed to evaluate the effect of chitosan combined with absorbable gelatin compressed sponge on the expression of osteoblastic differentiation marker genes during the healing of rat extraction socket. Materials and Methods: Twenty-four male Wistar rats were used. In control group, the extraction socket was closed with suture. In chitosan group, the socket was filled with chitosan combined with Gelfoam (Pharmacia & Upjohn Co.) and closed with suture. In each group, the animals were sacrificed at 3 days, 1 week, 2 weeks, and 4 weeks postoperatively. The expression of osteoblastic differentiation marker genes, including BSP, OCN, Runx2, and Col1 were quantified by real-time polymerase chain reaction. Result: Compared to control group, the mRNA level of BSP in chitosan group increased significantly at 2 weeks after extraction and the level of OCN decreased significantly at 3 days and 4 weeks after extraction (P<0.05). The mRNA levels of OCN, Runx2, and Col1 in chitosan group increased slightly at 2 weeks after extraction, but there was no statistical difference between groups. Conclusion: The results indicate that chitosan has some effects on the expression of osteogenic genes during the healing of extraction sockets.

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References

  1. Schropp L, Wenzel A, Kostopoulos L, Karring T. Bone healing and soft tissue contour changes following single-tooth extraction: a clinical and radiographic 12-month prospective study. Int J Periodontics Restorative Dent. 2003; 23: 313-23.
  2. Evian CI, Rosenberg ES, Coslet JG, Corn H. The osteogenic activity of bone removed from healing extraction sockets in humans. J Periodontol. 1982; 53: 81-5. https://doi.org/10.1902/jop.1982.53.2.81
  3. Cardaropoli G, Araujo M, Hayacibara R, Sukekava F, Lindhe J. Healing of extraction sockets and surgically produced--augmented and non-augmented--defects in the alveolar ridge. An experimental study in the dog. J Clin Periodontol. 2005; 32: 435-40. https://doi.org/10.1111/j.1600-051X.2005.00692.x
  4. Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat Res. 1986; (205): 299-308.
  5. Yen AH, Sharpe PT. Stem cells and tooth tissue engineering. Cell Tissue Res. 2008; 331: 359-72. https://doi.org/10.1007/s00441-007-0467-6
  6. Bonfield W. Designing porous scaffolds for tissue engineering. Philos Trans A Math Phys Eng Sci. 2006; 364: 227-32. https://doi.org/10.1098/rsta.2005.1692
  7. Casagrande L, Cordeiro MM, Nor SA, Nor JE. Dental pulp stem cells in regenerative dentistry. Odontology. 2011; 99: 1-7. https://doi.org/10.1007/s10266-010-0154-z
  8. Hutmacher DW, Schantz JT, Lam CX, Tan KC, Lim TC. State of the art and future directions of scaffold-based bone engineering from a biomaterials perspective. J Tissue Eng Regen Med. 2007; 1: 245-60. https://doi.org/10.1002/term.24
  9. Costa-Pinto AR, Reis RL, Neves NM. Scaffolds based bone tissue engineering: the role of chitosan. Tissue Eng Part B Rev. 2011; 17: 331-47. https://doi.org/10.1089/ten.teb.2010.0704
  10. Lesaffre E, Philstrom B, Needleman I, Worthington H. The design and analysis of split-mouth studies: what statisticians and clinicians should know. Stat Med. 2009; 28: 3470-82. https://doi.org/10.1002/sim.3634
  11. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001; 25: 402-8. https://doi.org/10.1006/meth.2001.1262
  12. Kronenberg HM. Developmental regulation of the growth plate. Nature. 2003; 423: 332-6. https://doi.org/10.1038/nature01657
  13. Robey PG, Termine JD. Human bone cells in vitro. Calcif Tissue Int. 1985; 37: 453-60. https://doi.org/10.1007/BF02557826
  14. Ripamonti U, Duneas N. Tissue engineering of bone by osteoinductive biomaterials. MRS Bulletin. 1996; 21: 36-9.
  15. Shin SY, Park HN, Kim KH, Lee MH, Choi YS, Park YJ, Lee YM, Ku Y, Rhyu IC, Han SB, Lee SJ, Chung CP. Biological evaluation of chitosan nanofiber membrane for guided bone regeneration. J Periodontol. 2005; 76: 1778-84. https://doi.org/10.1902/jop.2005.76.10.1778
  16. Suh JK, Matthew HW. Application of chitosan-based polysaccharide biomaterials in cartilage tissue engineering: a review. Biomaterials. 2000; 21: 2589-98. https://doi.org/10.1016/S0142-9612(00)00126-5
  17. Shi C, Zhu Y, Ran X, Wang M, Su Y, Cheng T. Therapeutic potential of chitosan and its derivatives in regenerative medicine. J Surg Res. 2006; 133: 185-92. https://doi.org/10.1016/j.jss.2005.12.013
  18. Madihally SV, Matthew HW. Porous chitosan scaffolds for tissue engineering. Biomaterials. 1999; 20: 1133-42. https://doi.org/10.1016/S0142-9612(99)00011-3
  19. Yeo YJ, Jeon DW, Kim CS, Choi SH, Cho KS, Lee YK, Kim CK. Effects of chitosan nonwoven membrane on periodontal healing of surgically created one-wall intrabony defects in beagle dogs. J Biomed Mater Res B Appl Biomater. 2005; 72: 86-93.
  20. Olson RA, Roberts DL, Osbon DB. A comparative study of polylactic acid, Gelfoam, and Surgicel in healing extraction sites. Oral Surg Oral Med Oral Pathol. 1982; 53: 441-9. https://doi.org/10.1016/0030-4220(82)90453-4
  21. Correll JT, Wise E. Certain properties of a new physiologically absorbable sponge. Exp Biol Med (Maywood). 1945; 58: 233-5. https://doi.org/10.3181/00379727-58-14908
  22. Rodan GA, Noda M. Gene expression in osteoblastic cells. Crit Rev Eukaryot Gene Expr. 1991; 1: 85-98.
  23. Lalani Z, Wong M, Brey EM, Mikos AG, Duke PJ. Spatial and temporal localization of transforming growth factor-beta1, bone morphogenetic protein-2, and platelet-derived growth factor-A in healing tooth extraction sockets in a rabbit model. J Oral Maxillofac Surg. 2003; 61: 1061-72. https://doi.org/10.1016/S0278-2391(03)00319-7
  24. Ganss B, Kim RH, Sodek J. Bone sialoprotein. Crit Rev Oral Biol Med. 1999; 10: 79-98. https://doi.org/10.1177/10454411990100010401
  25. Fisher LW, Fedarko NS. Six genes expressed in bones and teeth encode the current members of the SIBLING family of proteins. Connect Tissue Res. 2003; 44(Suppl 1): 33-40. https://doi.org/10.1080/713713644
  26. Waltregny D, Bellahcene A, de Leval X, Florkin B, Weidle U, Castronovo V. Increased expression of bone sialoprotein in bone metastases compared with visceral metastases in human breast and prostate cancers. J Bone Miner Res. 2000; 15: 834-43.
  27. Dhore CR, Cleutjens JP, Lutgens E, Cleutjens KB, Geusens PP, Kitslaar PJ, Tordoir JH, Spronk HM, Vermeer C, Daemen MJ. Differential expression of bone matrix regulatory proteins in human atherosclerotic plaques. Arterioscler Thromb Vasc Biol. 2001; 21: 1998-2003. https://doi.org/10.1161/hq1201.100229
  28. Bumgardner JD, Wiser R, Elder SH, Jouett R, Yang Y, Ong JL. Contact angle, protein adsorption and osteoblast precursor cell attachment to chitosan coatings bonded to titanium. J Biomater Sci Polym Ed. 2003; 14: 1401-9. https://doi.org/10.1163/156856203322599734
  29. Zhao F, Grayson WL, Ma T, Bunnell B, Lu WW. Effects of hydroxyapatite in 3-D chitosan-gelatin polymer network on human mesenchymal stem cell construct development. Biomaterials. 2006; 27: 1859-67. https://doi.org/10.1016/j.biomaterials.2005.09.031
  30. Leong KF, Cheah CM, Chua CK. Solid freeform fabrication of three-dimensional scaffolds for engineering replacement tissues and organs. Biomaterials. 2003; 24: 2363-78. https://doi.org/10.1016/S0142-9612(03)00030-9
  31. Stephan SJ, Tholpady SS, Gross B, Petrie-Aronin CE, Botchway EA, Nair LS, Ogle RC, Park SS. Injectable tissue-engineered bone repair of a rat calvarial defect. Laryngoscope. 2010; 120: 895-901.
  32. Ang TH, Sultana FSA, Hutmacher DW, Wong YS, Fuh JYH, Mo XM, Loh HT, Burdet E, Teoh SH. Fabrication of 3D chitosan-hydroxyapatite scaffolds using a robotic dispensing system. Mater Sci Eng C. 2002; 20: 35-42. https://doi.org/10.1016/S0928-4931(02)00010-3
  33. Lin HY, Chen JH. Osteoblast differentiation and phenotype expressions on chitosan-coated Ti-6Al-4V. Carbohydr Polym. 2013; 97: 618-26. https://doi.org/10.1016/j.carbpol.2013.05.048
  34. Chevrier A, Hoemann CD, Sun J, Buschmann MD. Chitosan-glycerol phosphate/blood implants increase cell recruitment, transient vascularization and subchondral bone remodeling in drilled cartilage defects. Osteoarthritis Cartilage. 2007; 15: 316-27. https://doi.org/10.1016/j.joca.2006.08.007
  35. Liu C, Wu Z, Sun HC. The effect of simvastatin on mRNA expression of transforming growth factorbeta1, bone morphogenetic protein-2 and vascular endothelial growth factor in tooth extraction socket. Int J Oral Sci. 2009; 1: 90-8. https://doi.org/10.4248/ijos.08011