Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Study on Anti-oxidant and Anti-inflammatory Activity of the Tissue-cultured Shoot Clumps from Raoulia australis

라울리아 신초 추출물의 항산화 및 항염 효능에 관한 연구

  • Park, Chang-Min (R&D Center, Hankook Cosmetics Manufacturing Co., Ltd.) ;
  • Han, Na-Kyeong (R&D Center, Hankook Cosmetics Manufacturing Co., Ltd.) ;
  • Joung, Min-Seok (R&D Center, Hankook Cosmetics Manufacturing Co., Ltd.) ;
  • Paek, Kee-Yoeup (Research Center for The Development of Advanced Horticultural Technology, Chungbuk National University) ;
  • Choi, Jong-Wan (R&D Center, Hankook Cosmetics Manufacturing Co., Ltd.)
  • 박창민 ((주)한국화장품제조 기술개발연구소) ;
  • 한나경 ((주)한국화장품제조 기술개발연구소) ;
  • 정민석 ((주)한국화장품제조 기술개발연구소) ;
  • 백기엽 (충북대학교 첨단원예기술개발센터) ;
  • 최종완 ((주)한국화장품제조 기술개발연구소)
  • Received : 2014.08.14
  • Accepted : 2014.12.15
  • Published : 2014.12.31
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Abstract

In this study, the shoot clumps extract of tissue-cultured Raoulia australis using the bioreactor culture system was tested for use a natural cosmetic ingredient. Tissue-cultured R. australis shoot clumps extract was tested anti-oxidant and anti-inflammatory activity for a cosmetic application. R. australis is a wild herbaceous plant of the asteraceae growing in New Zealand and Australia. Previous studies have reported anti-viral activity of the inhibitory effects for the growth of viruses induced meningitis, bronchitis and respiratory diseases but other biological effects are unknown. The shoot clumps extract of tissue-cultured R. australis showed higher anti-oxidant effect and anti-inflammatory effect than the natural R. australis extract. In DPPH, NBT and ABTS assay, the shoot clumps extract of tissue-cultured R. australis enhanced radical scavenging activity (up to 10~25% at $50{\mu}L/mL$) more than the natural R. australis extract. Also, the shoot clumps extract of tissue-cultured R. australis inhibited expression of iNOS and COX-2 protein in LPS-stimulated Raw 264.7 macrophages more than the natural R. australis extract. From this study, the shoot clumps extract of tissue-cultured R. australis displayed strong possibility as a new natural cosmetic ingredient for skin-care products.

본 연구에서는 생물반응장치를 이용하여 조직 배양된 라울리아 신초에 대하여 화장품 성분으로써 응용가치를 평가하였다. 조직 배양한 라울리아 신초에 대한 항산화 및 항염 활성 효과를 연구하였다. 라울리아는 뉴질랜드나 호주에서 자생하는 국화과의 야생초본식물이다. 이미 몇몇 보고 된 논문에서 라울리아는 기관지염, 수막염 그리고 호흡기 질병 등을 유발하는 바이러스에 대한 증식 억제 활성이 있다고 보고되었다. 실험 결과 조직배양된 라울리아 신초 추출물은 자연 상태의 라울리아 추출물과 비교하여 항산화 활성 및 항염 활성 효과가 우수하였다. 조직 배양된 라울리아 신초 추출물은 자연에서 자란 라울리아 추출물보다 $50{\mu}L/mL$ 농도에서 10~25% 항산화 활성을 증가시켰다. 또한 조직 배양된 라울리아 신초 추출물은 LPS로 유도된 대식세포에서 iNOS와 COX-2의 단백질 발현이 자연에서 자란 라울리아 추출물보다 억제되었다. 본 연구의 결과들로, 조직배양 한 라울리아 신초 추출물은 피부 보호를 위한 천연 화장품 성분으로써 우수한 가능성을 제공할 수 있을 것으로 사료된다.

Keywords

References

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