대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제40권4호
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  • Pages.341-347
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  • 2014
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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노무라입깃해파리 가수분해 추출물의 멜라닌합성 저해효과

Melanogenesis Inhibition Effects of Nemopilema nomurai Hydrolized Extracts

  • 이하연 ((주)내추럴솔루션 중앙연구소) ;
  • 김영훈 ((주)내추럴솔루션 중앙연구소) ;
  • 엄선영 ((주)내추럴솔루션 중앙연구소) ;
  • 정욱순 ((주)내추럴솔루션 중앙연구소) ;
  • 장문식 ((주)내추럴솔루션 중앙연구소) ;
  • 이남호 (제주대학교 화학.코스메틱스학부)
  • 투고 : 2014.07.11
  • 심사 : 2014.09.22
  • 발행 : 2014.12.31
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초록

본 연구에서는 노무라입깃해파리(Nemopilema nomurai) 가수분해 추출물의 미백활성에 대하여 검색하였다. 가수분해 시료(NHE, N. nomurai Hydrolized Extract)는 노무라입깃해파리를 상업용 단백질분해효소(Protamex, Alcalase, Flavourzyme, Neutrase)를 이용하여 $55^{\circ}C$에서 pH 5-8 조건으로 처리하는 과정을 거쳐 얻었다. 미백활성은 B16-F1 멜라노마 세포에서 멜라닌 색소 합성 저해능 평가를 통하여 확인하였다. 실험결과, Neutrase로 처리된 시료(N-NHE)에서 $100{\mu}g/mL$의 농도에서 멜라닌 생성이 가장 효율적으로 89.9% 감소되는 것을 관찰하였다. 이 시료는 또한 tyrosinase와 TRP-1 (tyrosinase related protein-1) 단백질의 발현을 억제하였다. 이상의 연구 결과는 노무라입깃해파리 가수분해 추출물의 화장품 미백 소재로의 개발 가능성을 보여주고 있다.

This study was aimed to investigate skin whitening effects of Nomura's jellyfish (Nemopilema nomurai) hydrolyzed extracts (NHE). The extracts were prepared through the hydrolysis of N. nomurai using commercial proteolytic enzymes such as Protamex, Alcalase, Flavourzyme and Neutrase with optimum pHs (pH 5-8) at $55^{\circ}C$. Their whitening activities were examined from the inhibition of melanin synthesis using B16-F1 cell lines. Among the examined samples, Neutrase-treated extract (N-NHE) showed the most significant inhibition effect on melanin synthesis by 89.9% at a concentration of $100{\mu}g/mL$. This sample decreased the expression of tyrosinase and TRP-1 (tyrosinase related protein-1) proteins as well. These results suggested the potential application of NHE as whitening ingredients in cosmetic preparation.

키워드

참고문헌

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