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The Morphological Characteristics of Pseudo-nitzschia cf. brasiliana Observed in Korea Strait, 2010

2010년 국내 남해 해역에 출현한 Pseudo-nitzschia cf. brasiliana의 형태 특성

  • Hur, Young Baek (National Fisheries Research & Development Institute/Southeast Sea Fisheries Research Institute) ;
  • Park, Jong-Gyu (Department of Marine Biotechnology, Kunsan National University)
  • 허영백 (국립수산과학원 남동해수산연구소) ;
  • 박종규 (국립국산대학교 해양생물공학과)
  • Received : 2014.11.26
  • Accepted : 2014.12.06
  • Published : 2014.12.31

Abstract

Several species of the genus Pseudo-nitzschia produce the neurotoxin domoic acid (DA) known to be responsible for amnesic shellfish poisoning. Recently off the coast of Geomundo and in Exclusive Economic Zone (EEZ) of Korea Strait, P. cf. brasiliana predominated in summer, 2010. As P. cf. brasiliana was new to Korea, we describe the morphological characteristics of the species. It was observed that cells were linear to lanceolate in valve and girdle view, $51{\sim}76{\mu}m$ long and $1.9{\sim}2.6{\mu}m$ wide. In the central part a large interspace was absent. The number of fibulae and striae in $10{\mu}m$ were 30~37 and 31~37. Each stria contained 2~3 rows of poroids, with 10 to 14 poroids in $1{\mu}m$ length. These morphological features of P. cf. brasiliana were found to be similar with the P. brasiliana, except with the fact that P. cf. brasiliana carred more numbers of fibulae/striae in $10{\mu}m$ and poroids in $1{\mu}m$. Based on our observation, we suggest that P. cf. brasiliana is a newly identified Pseudo-nitzschia species or a variety of P. brasiliana.

규조류 Pseudo-nitzschia는 아미노산의 일종 신경독인 기억상실성 패독 domoic aicd (DA)를 생산하여 주목받고 있는 속이다. 최근 2010년 여름철에 남해 EEZ 골재채취 단지와 거문도에서 Pseudo-nitzschia cf. brasiliana가 최고 우점종으로 출현하여 그 형태 특성을 관찰하였다. P. cf. brasiliana는 개각(valve)의 모양이 선형이었고, 길이와 폭은 각각 $51{\sim}76{\mu}m$, $1.9{\sim}2.6{\mu}m$이었으며 중간 공간(central interspace)은 없었다. $10{\mu}m$ 안의 등줄안다리(fibulae) 수와 점무늬열(striae) 수는 각각 30~37, 31~37이었다. 미소공(poroid)은 2~3줄이었으며, $1{\mu}m$안에 10~14개가 있었다. 이러한 형태 특성은 $10{\mu}m$ 안에 있는 등줄안다리(fibulae) 수와 점무늬열(striae)의 수, 미소공(Poroid) 수에서 P. brasiliana와 크게 차이가 났다. 따라서 P. cf. brasiliana는 P. brasiliana의 변종이나 신종으로 추정된다.

Keywords

References

  1. Amato A and M Montresor. 2008. Morphology, phylogeny, and sexual cycle of Pseudo-nitzschia mannii sp. nov. (Bacillariophyceae): a pseudo-cryptic species within the P. pseudo-delicatissima complex. Phycologia 47:487-497. https://doi.org/10.2216/07-92.1
  2. Bates SS, CJ Bird, ASW de Freitas, R Foxall, M Gilgan, LA Hanic, GR Johnson, AW McCulloch, P Odense, R Pocklington, MA Quilliam, PG Sim, JC Smith, DV Subba Rao, ECD Todd, JA Walter and JLC Wright. 1989. Pennate diatom Nitzschia pungens as the primary source of domoic acid, a toxin in shellfish from Eastern Prince Edward Island, Canada. Can. J. Fish. Aquat. Sci. 46:203-1215. https://doi.org/10.1139/f89-028
  3. Fryxell GA and GA Hasle. 2003. Taxonomy of harmful diatoms. In: Manual on harmful marine microalgae, edited by Hallegraeff, G.M., D.M. Anderson and A.D. Cembella, UNESCO, Paris, pp. 465-509.
  4. Hasle GR. 2002. Are most of the domoic acid-producing species of the diatom genus Pseudo-nitzschia cosmopolite? Harmful aglae 1:137-146. https://doi.org/10.1016/S1568-9883(02)00014-8
  5. Kim EK. 2010. A study on the pothentially toxic Pseudo-nitzschia species in Tongyeong coastal waters. Master Thesis of Kusnsan National Univ. 90pp. (in Korean)
  6. Korea Water Resources Corporation. 2010. Marie environmental impact statement on the marine aggregate extraction complex in EEZ of Korea Strait. 491pp. (in Korean)
  7. Lee JH and JH Baik. 1997. Neurotoxin-producing Pseudo-nitzschia multiseries (Hasle) Hasle, in the Coastal Waters of Southern Korea. II. Production of Domoic Acid. Algae 12:31-38. (in Korean)
  8. Lim H-C, C-P Leaw, S Nyun-Pau, S-T Teng, G Usup, N Mohammad-Noor, N Lundholm, Y Kotaki and P-T Lim. 2012. Morphology and molecular characterization of Pseudo-nitzschia (Bacillariophyceae) from Malaysian Borneo, including the new species Pseudo-nitzschia circumpora sp. nov. J. Phycol. 48:1232-1247. https://doi.org/10.1111/j.1529-8817.2012.01213.x
  9. Lim H-C, S-T Teng, C-P Leaw and P-T Lim. 2013. Three novel species in the Pseudo-nitzschia pseudodelicatissima complex: P. batesiana sp. nov., P. lundholmiae sp. nov., and P. fukuyoi sp. nov.(Bacillariophyceae) from the Strait of Malacca, Malaysia. J. Phycol. 49:902-916.
  10. Lundholm N and O Moestrup. 2002. The marine diatom Pseudo-nitzschia galaxiae sp. nov (Bacillariophyceae): morphology and phylogenetic relationships. Phycologia 41:594-605. https://doi.org/10.2216/i0031-8884-41-6-594.1
  11. Lundholm N, GR Hasle, GA Fryxell and PE Hargraves. 2002. Morphology, phylogeny and toxonomy of species within the Pseudo-nitzschia americana complex (Bacillariophyceae) with descriptions of two new species. Pseudo-nitzschia brasiliana and Pseudo-nitzschia linea. Phycologia 41:480-497. https://doi.org/10.2216/i0031-8884-41-5-480.1
  12. Lundholm N, O Moestrup, GR Hasle and K Hoef-Edmen. 2003. A study of the P. pseudodelicatissima/cuspidata complex (Bacillariophyceae): What is Pseudo-nitzschia pseudodelicatissima? J. Phycol. 39:797-813. https://doi.org/10.1046/j.1529-8817.2003.02031.x
  13. Lundholm N, O Moestrup, Y Kotaki, K Hoef-Emden, C Scholin and P Miller. 2006. Inter- and intraspecific variation of the Pseudo-nitzschia delicatissima-complex (Bacillariophyceae) illustrated by rRNA probes, morphological data and phylogenetic analyses. J. Phycol. 42:464-481. https://doi.org/10.1111/j.1529-8817.2006.00211.x
  14. Lundholm N, SS Bates, KA Baugh, BD Bill, LB Connell, C Leger and V Trainer. 2012. Cryptic and pseudo-cryptic diversity in diatoms - with descriptions of Pseudo-nitzschia hasleana sp. nov. and P. fryxelliana sp. nov. J. Phycol. 48:436-454. https://doi.org/10.1111/j.1529-8817.2012.01132.x
  15. Orive E, L Perez-Aicua, H David, K Garcia-Etxebarria, A Laza-Martinez, S Seoane and I Miguel. 2013. The genus Pseudo-nitzschia (Bacillariophycea) in a temperate estuary with description of two new species: Pseudo-nitzschia plurisecta sp. nov. and Pseudo-nitzschia abrensis sp. nov. J. Phycol. 49:1192-1206. https://doi.org/10.1111/jpy.12130
  16. Park J-G, EK Kim and W-A Lim. 2009. Potentially toxic Pseudo-nitzschia species in Tongyeong coastal waters, Korea. The Sea 14:163-170. (in Korean)
  17. Park J-G, ES Cho, HJ Hur, JK Son and TS Lee. 1999. Occurrence and rapid differentiation of potentially toxic Pseudo-nitzschia species in Chinhae Bay. In A Special Symposium on the Recent Approaches and Control Thechnique in Korean Red-tide Researches. pp. 70-84. (in Korean)
  18. Priisholm K, O Moestrup and N Lundholm. 2002. Taxonomic notes on the marine diatom genus Pseudo-nitzschia in the Andaman sea near the island of Phuket, Thailand, with a description of Pseudo-nitzschia micropora sp. nov. Diatom Res. 17:153-175. https://doi.org/10.1080/0269249X.2002.9705536
  19. Quijano-Scheggia S, E Garces, K Andree, J-M Fortuno and J Camp. 2009a. Homothallic auxosporulation in Pseudo-nitzschia brasiliana (Bacillariophyta). J. Phycol. 45:100-107. https://doi.org/10.1111/j.1529-8817.2008.00636.x
  20. Quijano-Scheggia S, E Garces, N Sampedro, KV Lenning, E Flo, K Andree, J-M Fortuno and J Camp. 2008. Identification and characterisation of the dominant Pseudo-nitzschia species (Bacillariophyceae) along the NE Spanish coast (Catalonia, NW Mediterranean). Sci. Mar. 72:343-359.
  21. Quijano-Scheggia SI, E Garces, N Lundholm, O Moestrup, K Andree and J Camp. 2009b. Morphology, physiology, molecular phylogeny and sexual compatibility of the cryptic Pseudo-nitzschia delicatissima complex (Bacillariophyta), including the description of P. arenysensis sp. nov. Phycologia 48:492-509. https://doi.org/10.2216/08-21.1
  22. Skov J, N Lundholm, O Moestrup and J Larsen. 1999. Potentially Toxic Phytoplankton 4. the diatom genus Pseudo-nitzschia (Diatomphyceae/Bacillariophyceae). ICES Identification Leaflets for plankton. LEAFLET NO. 185. ISSN 1019-1097.
  23. Todd ECD. 1993. Domoic acid and amnesic shellfish poisoning - a review. J. Food Protect. 56:69-83.
  24. Tomas CR. 1997. Identifying marine phytoplankton. Academi press. pp. 307-324.
  25. Wang P, J Liang, X Lin, C Chen, Y Huang, Y Gao and Y Gao. 2012. Morphology, phylogeny and ITS-2 secondary structure of Pseudo-nitzschia brasiliana (Bacillariophyceae), including Chinese strains. Phycologia 51:1-10. https://doi.org/10.2216/09-108.1
  26. Wright JL, RK Boyd, ASW de Freitas, M Falk, RA Foxall, WD Hamieson, MV Laycock, AW McCulloch, AG McInnes, P Odense, VP Pathak, MA Quilliam, MA Ragan, PG Sim, P Thibault, JA Walter, M Gilgan, DJA Richard and D Dewar. 1989. Identification of domic acid, a neuroexcitatory amino acid, in toxic mussels from eastern Prince Edward Island. Can. J. Chem. 67:481-490. https://doi.org/10.1139/v89-075