International Journal of Oral Biology

  • 제39권4호
  • /
  • Pages.215-220
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  • 2014
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  • 1226-7155(pISSN)
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  • 2287-6618(eISSN)
대한구강생물학회 (The Korean Academy of Oral Biology)
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Molecular Identification of Bacterial Species Present on Toothbrushes

  • Kwon, Ye Won (Department of Microbiology and Immunology, College of Dentistry, Research Institute of Oral Science, Gangneung-Wonju National University) ;
  • Lee, Si Young (Department of Microbiology and Immunology, College of Dentistry, Research Institute of Oral Science, Gangneung-Wonju National University)
  • 투고 : 2014.11.19
  • 심사 : 2014.12.09
  • 발행 : 2014.12.31
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초록

Toothbrushes play an essential role in oral hygiene. However, they can be significant in microbial transmission and can increase the risk of infection, since they can serve as a reservoir for microorganisms in healthy, oral-diseased and medically ill adults. This study was conducted to evaluate toothbrush contamination in six toothbrushes donated from four people. Two participants each supplied two toothbrushes - one used in the bathroom and one used in the workplace. The other two people each donated two toothbrushes used in the workplace. Polymerase chain reaction was used to construct a 16S rRNA clone library. Sequences of cloned DNA were compared with those from the reference organisms provided by GenBank. A total 120 clones, representing 20 clones for each toothbrush, were analyzed. They are composed of six pylum, 46 genera and 79 species. The most dominant species were Streptococcus oralis, Streptococcus parasanguinis and Haemophilus parainfluenzae. Enterobacter and Escherichia were recovered from toothbrushes used domestically. Toothbrushes used in the workplace did not contain Enterobacteria.

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참고문헌

  1. Ho HP, Niederman R. Effectiveness of the sonicare sonic toothbrush on reduction of plaque, gingivitis, probing pocket depth and subgingival bacteria in adolescent orthodontic patients. J Clin Dent. 1997;8:15-19.
  2. Rosema NA, Timmerman MF, Versteeg PA, van Palenstein Helderman WH, Van der Velden U, Van der Weijden GA. Comparison of the use of different modes of mechanical oral hygiene in prevention of plaque and gingivitis. J Periodontol. 2008;79:1386-1394. https://doi.org/10.1902/jop.2008.070654
  3. Creeth JE, Gallagher A, Sowinski J, Bowman J, Barrett K, Lowe S, Patel K, Bosma ML. The effect of brushing time and dentifrice on dental plaque removal in vivo. J Dent Hyg. 2009;83:111-116.
  4. Bonten MJ, Hayden MK, Nathan C, van Voorhis J, Matushek M, Slaughter S, Rice T, Weinstein RA. Epidemiology of colonisation of patients and environment with vancomycin-resistant enterococci. Lancet. 1996;348:1615-16159. https://doi.org/10.1016/S0140-6736(96)02331-8
  5. Caudry SD, Klitorinos A, Chan EC. Contaminated toothbrushes and their disinfection. J Can Dent Assoc. 1995;61:511-516.
  6. Glass RT, Jensen HG. More on the contaminated toothbrush: The viral story. Quintessence Int. 1988;19:713-716.
  7. Malmberg E, Birkhed D, Norvenius G, Noren JG, Dahlen G. Microorganisms on toothbrushes at day-care centers. Acta Odontol Scand. 1994;52:93-98. https://doi.org/10.3109/00016359409029061
  8. Muller HP, Lange DE, Muller RF. Actinobacillus actinomycetemcomitans contamination of toothbrushes from patients harbouring the organism. J Clin Periodontol. 1989;16:388-390. https://doi.org/10.1111/j.1600-051X.1989.tb00010.x
  9. Taji SS, Rogers AH. ADRF trebitsch scholarship. the microbial contamination of toothbrushes. A pilot study. Aust Dent J. 1998;43:128-130. https://doi.org/10.1111/j.1834-7819.1998.tb06101.x
  10. Verran J, Leahy-Gilmartin AA. Investigations into the microbial contamination of toothbrushes. Microbios. 1996;85:231-238.
  11. Glass RT, Lare MM. Toothbrush contamination: A potential health risk? Quintessence Int. 1986;17:39-42.
  12. Glass RT. The infected toothbrush, the infected denture, and transmission of disease: A review. Compendium. 1992;13:592, 594, 596-598.
  13. Dahlen G, Lindhe J, Sato K, Hanamura H, Okamoto H. The effect of supragingival plaque control on the subgingival microbiota in subjects with periodontal disease. J Clin Periodontol. 1992;19:802-809. https://doi.org/10.1111/j.1600-051X.1992.tb02174.x
  14. Axelsson P, Lindhe J, Nystrom B. On the prevention of caries and periodontal disease. results of a 15-year longitudinal study in adults. J Clin Periodontol. 1991;18:182-189. https://doi.org/10.1111/j.1600-051X.1991.tb01131.x