International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Comparison of Various Transfection Methods in Human and Bovine Cultured Cells

  • Jin, Longxun (Cellular Reprogramming & Embryo Biotechnology Laboratory, Dental Research Institute and School of Dentistry, Seoul National University) ;
  • Kim, Daehwan (Cellular Reprogramming & Embryo Biotechnology Laboratory, Dental Research Institute and School of Dentistry, Seoul National University) ;
  • Roh, Sangho (Cellular Reprogramming & Embryo Biotechnology Laboratory, Dental Research Institute and School of Dentistry, Seoul National University)
  • Received : 2014.09.15
  • Accepted : 2014.11.24
  • Published : 2014.12.31
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Abstract

Transfection is a gene delivery tool that is a popular means of manipulating cellular properties, such as induced pluripotent stem cell (iPSC) generation by reprogramming factors (Yamanaka factors). However, the efficiency of transfection needs to be improved. In the present study, three transfection protocols - non-liposomal transfection (NLT), magnetofection and electroporation - were compared by analysis of their transfection efficiencies and cell viabilities using human dental pulp cells (hDPC) and bovine fetal fibroblasts (bFF) as cell sources. Enhanced green fluorescent protein gene was used as the delivery indicator. For magnetofection, Polymag reagent was administrated. NLT, FuGENE-HD and X-treme GENE 9 DNA transfection reagents were used for NLT. For electroporation, the $Neon^{TM}$ and $NEPA21^{TM}$ electroporators were tested. $Neon^{TM}$ electroporation showed highest transfection efficiency when compared with NLT, magnetofection, and $NEPA21^{TM}$ electroporation, with transfection efficiency of about 33% in hDPC and 50% in bFF, based on viable cell population in each cell type. These results suggest that transfection by $Neon^{TM}$ electroporation can be used to deliver foreign genes efficiently in human and bovine somatic cells.

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References

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