Environmental Analysis Health and Toxicology

  • Volume 29
  • /
  • Pages.19.1-19.10
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  • 2014
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  • 2671-9525(eISSN)
The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
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Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in Saccharomyces cerevisiae

  • Nguyen, Ngoc-Tu (Department of Bioprocess Engineering, Chonbuk National University) ;
  • Kim, Yang-Hoon (Department of Microbiology, Chungbuk National University) ;
  • Bang, Seung Hyuck (Department of Bioprocess Engineering, Chonbuk National University) ;
  • Hong, Ji Hye (Mine Reclamation Corporation, Coal Center) ;
  • Kwon, Soon Dong (Mine Reclamation Corporation, Coal Center) ;
  • Min, Jiho (Department of Bioprocess Engineering, Chonbuk National University)
  • Received : 2014.07.16
  • Accepted : 2014.09.29
  • Published : 2014.01.01
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Abstract

Objectives Lysosome is the cell-organelle which is commonly used as biomonitoring tool in environmental pollution. In this study, the lysosomal proteomic of the yeast Saccharomyces cerevisiae was analyzed for utilization in the detection of toxic substances in mine water samples. Methods This work informs the expression of lysosomal proteomic in yeast in response with toxic chemicals, such as sodium meta-arsenite and tetracycline, for screening specific biomarkers. After that, a recombinant yeast contained this biomarker were constructed for toxic detection in pure toxic chemicals and mine water samples. Results Each chemical had an optimal dose at which the fluorescent protein intensity reached the peak. In the case of water samples, the yeast showed the response with sample 1, 3, 4, and 5; whereas there is no response with sample 2, 6, and 7. Conclusions The recombinant yeast showed a high ability of toxic detection in response with several chemicals such as heavy metals and pharmaceuticals. In the case of mine water samples, the response varied depending on the sample content.

Keywords

References

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