IMMUNE NETWORK

  • 제14권6호
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  • Pages.328-332
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  • 2014
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  • 1598-2629(pISSN)
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  • 2092-6685(eISSN)
대한면역학회 (The Korean Association of Immunobiologists)
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DOI QR코드

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Evidence for Direct Inhibition of MHC-Restricted Antigen Processing by Dexamethasone

  • Im, Sun-A (College of Pharmacy, Chungbuk National University) ;
  • Gerelchuluun, Turmunkh (Department of Biotechnology and Nutrition School of Industrial Technology, Mongolian University of Science and Technology) ;
  • Lee, Chong-Kil (College of Pharmacy, Chungbuk National University)
  • 투고 : 2014.10.13
  • 심사 : 2014.12.05
  • 발행 : 2014.12.31
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초록

Dexamethasone (Dex) was shown to inhibit the differentiation, maturation, and antigen-presenting function of dendritic cells (DC) when added during DC generation or maturation stages. Here, we examined the direct effects of Dex on MHC-restricted antigen processing. Macrophages were incubated with microencapsulated ovalbumin (OVA) in the presence of different concentrations of Dex for 2 h, and the efficacy of OVA peptide presentation was evaluated using OVA-specific CD8 and CD4 T cells. Dex inhibited both class I- and class II-restricted presentation of OVA to T cells; this inhibitory effect on antigen presentation was much more potent in immature macrophages than in mature macrophages. The presentation of the exogenously added OVA peptide SIINFEKL was not blocked by Dex. In addition, short-term treatment of macrophages with Dex had no discernible effects on the phagocytic activity, total expression levels of MHC molecules or co-stimulatory molecules. These results demonstrate that Dex inhibits intracellular processing events of phagocytosed antigens in macrophages.

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참고문헌

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