Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Immune Modulation of B. terrestris Worker (a Type of Bumblebee), Extract on CFA-induced Paw Edema in Rats

  • Ahn, Mi Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Kim, Soon Ja (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Han, Jea Woong (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Yoon, Hyung Joo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Hwang, Jae Sam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA) ;
  • Yun, Eun Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
  • Received : 2014.10.02
  • Accepted : 2014.11.19
  • Published : 2014.12.31
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Abstract

To develop a composition for enhancing immunity, based on alcohol extracts of the bumblebee as an active ingredient, bumblebee ethanol extracts were evaluated for their protective effect in chronic models of inflammation, adjuvant induced rat arthritis. B. terrestris worker extract (SDIEX) and, B. hypocrita sapporoensis lava and pupa extract (SPDYBEX), significantly decreased paw edema in arthritic rats, at a dose 100 mg/kg, respectively. The cytokine levels related inflammation of COX-2, $sPLA_2$, VEGF, and TNF-${\alpha}$, were decreased, compared to positive control, indomethacin (5 mg/kg). Histopathological data demonstrated decreases inflammatory activity, hind paw edema, and repaired hyaline articular cartilage in DRG over a 2 wk administration. HPLC and GC-MS analysis of SDIEX and SPDYBEX revealed the presence of cantharidin.

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References

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