Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Anti-inflammatory Effects of Cheongnoimyungshin-hwan in Microglia Cells

미세아교세포의 염증반응에 미치는 청뇌명신환의 영향

  • Im, Yong-Gyun (Department of Oriental Medicine Graduate School, Dong-Eui University) ;
  • Choi, Yung-Hyun (Department of Oriental Medicine Graduate School, Dong-Eui University) ;
  • Hwang, Won-Deok (Department of Oriental Medicine Graduate School, Dong-Eui University)
  • 임용균 (동의대학교 한의과대학 대학원) ;
  • 최영현 (동의대학교 한의과대학 대학원) ;
  • 황원덕 (동의대학교 한의과대학 대학원)
  • Received : 2014.12.05
  • Accepted : 2014.12.18
  • Published : 2014.12.30
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Abstract

Objectives: Activated microglia cells play an important role in inflammatory responses in the central nervous system (CNS) which are involved in neurodegenerative diseases. We attempted to determine the anti-inflammatory effects of Cheongnoimyungshin-hwan (CNMSH) in microglia cells. Methods: We examined the effect of CNMSH on the inflammatory responses in BV2 microglia cells induced by lipopolysaccharide (LPS) and explored the mechanism underlying the action of CNMSH. Results: BV2 cells treated with LPS showed an up-regulation of nitric oxide (NO), prostaglandin $PGE_2(PGE_2)$ and interleukin $1{\beta}(IL-1{\beta})$ release, whereas CNMSH suppressed this up-regulation. CNMSH inhibited the induction of COX-2, iNOS and $IL-1{\beta}$ proteins in LPS-treated BV2 cells and blocked the LPS-induced phosphorylation and nuclear translocation of nuclear factor ${\kappa}B(NF-{\kappa}B$). Furthermore, CNMSH attenuated the LPS-induced phosphorylation of extracellular signal-regulated kinase and p38 mitogen activated protein kinase (MAPK), as well as the phosphoinositide 3-kinase (PI3K)/Akt signaling pathway, but did not inhibit the LPS-induced phosphorylation of c-Jun amino terminal kinase. Conclusions: These results suggest that the inhibitory effect of CNMSH on the LPS-induced production of inflammatory mediators and cytokines in BV2 cells is associated with the suppression of the $NF-{\kappa}B$ and PI3KAkt signaling pathways.

Keywords

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