Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Lifespan Extension of Fermented Zizyphus jujuba Fruits in Caenorhabditis elegans

붉은 덕다리버섯 발효 대조(大棗)의 예쁜꼬마선충 수명연장효과

  • Ji, Byeong-Uk (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Park, Sung-Min (CoSeed BioPharm Corporation) ;
  • Koo, Sungtae (Division of Meridian and Structural Medicine, School of Korean Medicine, Pusan National University) ;
  • Lim, Byungmook (Division of Humanities and Social Medicine, School of Korean Medicine, Pusan National University) ;
  • Yu, Young-Beob (Department of Herbal Pharmaceutical Development, Nambu University)
  • 지병욱 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 박성민 ((주)코씨드바이오팜) ;
  • 구성태 (부산대학교 한의학전문대학원 경락구조의학부) ;
  • 임병묵 (부산대학교 한의학전문대학원 인문사회의학부) ;
  • 유영법 (남부대학교 한방제약개발학과)
  • Received : 2014.12.06
  • Accepted : 2014.12.19
  • Published : 2014.12.27
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Abstract

Objectives : Zizyphus jujuba fruits(ZJF), a traditional Korean medicine has various biological activities such as anti-inflammatory, anti-oxidative and neuro-protective effects. However, it is still unclear whether ZJF has any biological effect on anti-aging. In this study, we examined the effect of ZJF on lifespan and thermal stress in C. elegans. Methods and Results : ZJF water extracts were fermented for 7 days(F7-ZJF) and 14 days(F14-ZJF) by Laetiporus sulphureus to increase secondary metabolites such as aglycone of flavonoids and terpenoids. In the lifespan assay, ZJF water extracts and fermented ZJF were treated on the agar medium plate with age synchronized egg stage of C. elegans. Treatment of F7-ZJF-$200{\mu}g/mL$ with OP-50 E. coli and F14-ZJF-$200{\mu}g/mL$ with OP-50 E. coli significantly increased life span of C. elegans(N2) at thermal stress condition of $25^{\circ}C$. Moreover mRNA levels of lifespan associated HSP 16.1, HSP 70, and HSF-1 were increased at thermal stress condition of $25^{\circ}C$. However, in the equilibration temperature of $20^{\circ}C$ after stress condition of $35^{\circ}C$ for 2 hr, F-14-ZJF-$200{\mu}g/mL$ treatment decreased the levels of heat shock protein in hsp16.2/GFP C. elegans. Conclusions : Our study indicates that prolong role of fermented-ZJF in C. elegans is mediated by control HSPs production.

Keywords

References

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