Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Isolation and Identification of Adenosine and Phlomuroside from the Aerial Parts of Oryza sativa L.

벼(Oryza sativa L.)의 지상부로부터 adenosine과 phlomuroside의 분리 및 동정

  • Jeong, Rak-Hun (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Dae-Young (Herbal Crop Utilization Research Team, National Institute of Horticultural and Herbal Science, RDA) ;
  • Cho, Jin-Gyeong (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Baek, Yoon-Su (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Seo, Kyeong-Hwa (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Lee, Dong-Geol (R&D center, GFC Co., Ltd.) ;
  • Kang, Hee-Cheol (R&D center, GFC Co., Ltd.) ;
  • Kim, Ji-Young (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University) ;
  • Baek, Nam-In (Department of Oriental Medicinal Materials & Processing and Graduate School of Biotechnology, Kyung Hee University)
  • Received : 2014.04.15
  • Accepted : 2014.06.10
  • Published : 2014.12.31
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Abstract

Fresh and chopped aerial parts of Oryza sativa were extracted in 80% aqueous mehthanol, and the concentrated extract was successively partitioned in n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and $H_2O$ fractions. From the n-BuOH fraction, two compounds were isolated through repeated silica gel and ODS column chromatography (c.c.). Based on nuclear magnetic resonance (NMR), mass spectrometry and infrared spectroscopy spectroscopic data, the compounds were identified to be adenosine (1) and phlomuroside (2). Especially, the configuration of both the anomer hydroxyl groups was determined as ${\beta}$ from the coupling constants of the anomer protons (J =6.0 and 7.6 Hz) in the $^1H-NMR$ spectra. This is the first report for the isolation of these compounds from Oryza sativa L.

벼 지상부를 MeOH 수용액으로 추출하고 이 추출물을 n-hexane, EtOAc, n-BuOH, 및 $H_2O$ 분획으로 나누었다. n-BuOH 분획에 대하여 silica gel, ODS 및 Sephadex LH-20 column chromatography를 반복실시 하여 2종의 화합물을 분리하였다. NMR, IR 및 MS data를 해석하여 각각 adenosine (1)과 phlomuroside (2)로 구조 동정 하였다. 이들 화합물들은 벼에서는 처음으로 분리되었다.

Keywords

References

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