Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Siberian Sturgeon Oocyte Extract Induces Epigenetic Modifications of Porcine Somatic Cells and Improves Developmental Competence of SCNT Embryos

  • Kim, So-Young (Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Kim, Tae-Suk (Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Park, Sang-Hoon (Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Lee, Mi-Ran (Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Eun, Hye-Ju (Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Baek, Sang-Ki (Department of Animal Bioscience, College of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Ko, Yeoung-Gyu (Animal Genetic Resources Station, National Institute of Animal Science, Rural Development Administration) ;
  • Kim, Sung-Woo (Animal Genetic Resources Station, National Institute of Animal Science, Rural Development Administration) ;
  • Seong, Hwan-Hoo (Animal Genetic Resources Station, National Institute of Animal Science, Rural Development Administration) ;
  • Campbell, Keith H.S. (Animal Development and Biotechnology Group, Department of Animal Science, School of Biosciences, The University of Nottingham) ;
  • Lee, Joon-Hee (Institute of Agriculture and Life Science, College of Agriculture and Life Sciences, Gyeongsang National University)
  • Received : 2013.11.07
  • Accepted : 2013.11.29
  • Published : 2014.02.01
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Abstract

Somatic cell nuclear transfer (SCNT) has generally demonstrated that a differentiated cell can convert into a undifferentiated or pluripotent state. In the SCNT experiment, nuclear reprogramming is induced by exposure of introduced donor nuclei to the recipient cytoplasm of matured oocytes. However, because the efficiency of SCNT still remains low, a combination of SCNT technique with the ex-ovo method may improve the normal development of SCNT embryos. Here we hypothesized that treatment of somatic cells with extracts prepared from the germinal vesicle (GV) stage Siberian sturgeon oocytes prior to their use as nuclear donor for SCNT would improve in vitro development. A reversible permeability protocol with $4{\mu}g/mL$ of digitonin for 2 min at $4^{\circ}C$ in order to deliver Siberian sturgeon oocyte extract (SOE) to porcine fetal fibroblasts (PFFs) was carried out. As results, the intensity of H3K9ac staining in PFFs following treatment of SOE for 7 h at $18^{\circ}C$ was significantly increased but the intensity of H3K9me3 staining in PFFs was significantly decreased as compared with the control (p<0.05). Additionally, the level of histone acetylation in SCNT embryos at the zygote stage was significantly increased when reconstructed using SOE-treated cells (p<0.05), similar to that of IVF embryos at the zygote stage. The number of apoptotic cells was significantly decreased and pluripotency markers (Nanog, Oct4 and Sox2) were highly expressed in the blastocyst stage of SCNT embryos reconstructed using SOE-treated cells as nuclear donor (p<0.05). And there was observed a better development to the blastocyst stage in the SOE-treated group (p<0.05). Our results suggested that pre-treatment of cells with SOE could improve epigenetic reprogramming and the quality of porcine SCNT embryos.

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References

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