Maxillofacial Plastic and Reconstructive Surgery

Korean Association of Maxillofacial Plastic and Reconstructive Surgeons (대한악안면성형재건외과학회)
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Effectiveness of Woven Silk Dressing Materials on Full-skin Thickness Burn Wounds in Rat Model

  • Lee, Woo-Young (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Um, In Chul (Department of Bio-fibers and Materials Science, College of Agriculture and Life Science, Kyungpook National University) ;
  • Kim, Min-Keun (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Kwon, Kwang-Jun (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Kim, Seong-Gon (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University) ;
  • Park, Young-Wook (Department of Oral and Maxillofacial Surgery, College of Dentistry, Gangneung-Wonju National University)
  • Received : 2014.10.03
  • Accepted : 2014.11.04
  • Published : 2014.11.30
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Abstract

Purpose: This study evaluated woven silk textile for burn wound dressing materials in an animal model. Methods: Ten rats were used in this experiment. Full-thickness $2{\times}2cm$ burn wounds were created on the back of the rats under anesthesia. In the experimental group, the wounds were treated with three different dressing materials from woven silk textile. In the control group, natural healing without any dressing material was set as control. The wound surface area was measured at five days, seven days, and 14 days. Wound healing was evaluated by histologic analysis. Results: There were no statistically significant differences among groups at five days post injury. The mean defect size at seven days was largest in Group 3 ($462.87mm^2$), and smallest in Group 1 ($410.89mm^2$), not a significant difference (P=0.341). The mean defect size at 14 days was smallest at the Group 3 ($308.28mm^2$) and largest in the control group ($388.18mm^2$), not a significant difference (P=0.190). The denuded area was smaller in Group 1 ($84.57mm^2$) and Group 2 ($82.50mm^2$) compared with the control group ($195.93mm^2$), not statistically significant differences (P=0.066, 0.062). The difference between Group 3 and control was also not statistically significant (P=0.136). In histologic analysis, the experimental groups re-epithelialized more than control groups. No evidence was found of severe inflammation. Conclusion: The healing of burn wounds was faster with silk weave textile more than the control group. There was no atypical inflammation with silk dressing materials. In conclusion, silk dressing materials could be used to treat burn wounds.

Keywords

References

  1. Kanokpanont S, Damrongsakkul S, Ratanavaraporn J, Aramwit P. Physico-chemical properties and efficacy of silk fibroin fabric coated with different waxes as wound dressing. Int J Biol Macromol 2013;55:88-97. https://doi.org/10.1016/j.ijbiomac.2013.01.003
  2. Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res 2010;89:219-29. https://doi.org/10.1177/0022034509359125
  3. Gosselin RA, Kuppers B. Open versus closed management of burn wounds in a low-income developing country. Burns 2008;34:644-7. https://doi.org/10.1016/j.burns.2007.09.013
  4. Neville BW, editor. Oral & maxillofacial pathology. 2nd ed. Philadelphia: W.B. Saunders; 2002.
  5. Kim MK, Yoo KY, Kwon KJ, et al. Powdered wound dressing materials made from wild silkworm Antheraea pernyi silk fibroin on full-skin thickness burn wounds on rats. Maxillofac Plast Reconstr Surg 2014;36:111-5. https://doi.org/10.14402/jkamprs.2014.36.3.111
  6. Panilaitis B, Altman GH, Chen J, Jin HJ, Karageorgiou V, Kaplan DL. Macrophage responses to silk. Biomaterials 2003;24:3079-85. https://doi.org/10.1016/S0142-9612(03)00158-3
  7. Horan RL, Antle K, Collette AL, et al. In vitro degradation of silk fibroin. Biomaterials 2005;26:3385-93. https://doi.org/10.1016/j.biomaterials.2004.09.020
  8. Kim HJ, Um IC. Effect of degumming ratio on wet spinning and post drawing performance of regenerated silk. Int J Biol Macromol 2014;67:387-93. https://doi.org/10.1016/j.ijbiomac.2014.03.055
  9. Kim HJ, Chung DE, Um IC. Effect of processing conditions on the homogeneity of partially degummed silk evaluated by FTIR spectroscopy. Int J Indust Entomol 2013;26:54-60. https://doi.org/10.7852/ijie.2013.26.1.054
  10. Minoura N, Tsukada M, Nagura M. Physico-chemical properties of silk fibroin membrane as a biomaterial. Biomaterials 1990;11:430-4. https://doi.org/10.1016/0142-9612(90)90100-5
  11. Kim KH, Jeong L, Park HN, et al. Biological efficacy of silk fibroin nanofiber membranes for guided bone regeneration. J Biotechnol 2005;120:327-39. https://doi.org/10.1016/j.jbiotec.2005.06.033
  12. Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH. Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 2004;25:1289-97. https://doi.org/10.1016/j.biomaterials.2003.08.045
  13. Minoura N, Aiba S, Higuchi M, Gotoh Y, Tsukada M, Imai Y. Attachment and growth of fibroblast cells on silk fibroin. Biochem Biophys Res Commun 1995;208:511-6. https://doi.org/10.1006/bbrc.1995.1368
  14. Lovett M, Cannizzaro C, Daheron L, Messmer B, Vunjak-Novakovic G, Kaplan DL. Silk fibroin microtubes for blood vessel engineering. Biomaterials 2007;28:5271-9. https://doi.org/10.1016/j.biomaterials.2007.08.008
  15. Cai ZX, Mo XM, Zhang KH, et al. Fabrication of chitosan/silk fibroin composite nanofibers for wound-dressing applications. Int J Mol Sci 2010;11:3529-39. https://doi.org/10.3390/ijms11093529
  16. Wang X, Kim HJ, Xu P, Matsumoto A, Kaplan DL. Biomaterial coatings by stepwise deposition of silk fibroin. Langmuir 2005;21:11335-41. https://doi.org/10.1021/la051862m

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