Antioxidant and Sensory Properties of Hot Water Extract of Liriope Tubers treated at Various Preprocess

전처리방법에 따른 맥문동 열수 추출물의 항산화성과 관능 특성

  • Yang, Mi-Ok (Dept. of Oriental Medicine & Healthcare, Wonkwang Digital University)
  • 양미옥 (원광디지털대학교 한방건강학과)
  • Received : 2013.09.11
  • Accepted : 2013.10.31
  • Published : 2013.10.31

Abstract

The results of examining total soluble solid, reducing sugar, antioxidant and sensory properties regarding LTD (Liriope Tuber Dried), LTSD (Liriope Tuber Steamed and Dried), LTASD (Liriope Tuber Alcohol-Steamed and Dried), LTDR (LTD Roasted), LTSDR (LTSD Roasted) and LTASDR (LTASD Roasted) are as follows : Total soluble solid content of the roasted samples (LTDR, LTSDR and LTASDR) was more than those of all dried samples (LTD, LTSD and LTASD). According to roasting conditions, total sugar and reducing sugar are significantly greater than the raw and dried sample (LTD) in all heat-treated samples. The browning index was significantly higher in all roasted samples. In particular, LTASDR had a high browning index. Further, the antioxidative activity of the roasted LT samples were higher than that of all dried LT samples. In particular, the LTASDR sample showed significantly high figures in DPPH scavenging activity, ABTS scavenging activity, Nitrite scavenging activity and xanthine oxidase inhibitory activity. Sensory properties showed an increased acceptance in all evaluation items among roasted samples. In this study, hot water extracts of steamed or alcohol-steamed roasted LT samples had a higher antioxidative effect than that of LTD or LTDR and attained positive results by getting high scores in the overall sensory evaluation. Therefore, when using Liriope tuber in making beverages or herbal recipes, it is appropriate to dry and roast before steaming or spreading with alcohol when treating LT.

생(生), 증(蒸), 주증(酒蒸) 건조 맥문동(LTD, LTSD, LTASD)과 생(生), 증(蒸), 주증(酒蒸) 건조 볶음 맥문동 시료(LTDR, LTSDR, LTASDR)의 가용성 고형물, 전당, 환원당과 항산화 효과, 관능평가를 실시한 결과는 다음과 같았다. 가용성 고형분 함량은 건조 후 볶음 처리한 시료(LTDR, LTSDR, LTASDR)가 모든 건조 맥문동 시료(LTD, LTSD, LTASD)보다 많았다. 전당과 환원당은 볶음 유무에 따라서 모든 열처리한 시료가 생건조 시료(LTD)와 비교하여 유의적으로 함량이 많았으며, 환원당은 특히 주증건조 볶음 시료(LTASDR)가 가장 많은 것으로 나타났다. 일정온도에서 환원당의 함량이 증가하는 이유는 열분해로 인한 저분자량의 올리고당이나 덱스트린 등의 환원당 생성량이 소모되는 양보다 현저히 많기 때문이다. 갈변도는 볶음 처리를 함에 따라 모든 볶음 맥문동 시료가 유의적으로 높은 값을 나타내었다. 특히 주증 건조볶음(LTASDR)의 갈변도가 높았는데, 주증(酒蒸)할 때에 이용된 청주에 함유되어 있는 당이 맥문동에 흡수되었고, 이에 따라 갈변도가 높아졌을 뿐 아니라, 항산화와 관련된 갈색 반응이 더 활발하게 일어나고, Maillard 반응의 중간생성물이 증가되어 여러 가지 항산화 효과가 나타났다. 항산화 활성 실험에서도 모든 건조 맥문동에 비하여 볶음 맥문동 시료의 항산화 활성이 높게 측정되었으며, 특히 주증 건조 볶음 시료(LTASDR)가 DPPH 라디칼 소거능, ABTs 라디칼 소거능, 아질산염 소거능, XOase 저해활성에 있어서 월등히 높은 수치를 나타내었다. 주증건조 볶음 시료(LTASDR)가 생건조 맥문동(LTD)의 열수 추출물에 비하여 항산화 효과가 대체적으로 유의적으로 높게 측정된 결과는 술로 찌고, 볶는 과정 중에 갈변물질이 더 많이 생성되었고, 수용성 성분 추출이 용이하도록 조직이 변화된 결과로 사료된다. 색(color), 향미(flavor), 단맛(sweetness), 구수한 맛(tasty flavor), 전반적인 기호도(overall acceptance)는 전체적으로 볶음 처리를 함에 따라 유의적인 차이로 기호도가 향상되었으며, 볶음 처리한 시료 간에는 유의적인 차이가 없었다. 본 연구를 통해 찌거나 주증 처리하여 볶은 맥문동 시료의 열수 추출물이 생건조 맥문동(LTD)이나 생건조 볶음 맥문동(LTDR)에 비해 항산화성이 높게 측정되었음을 확인하였고, 전체적인 관능평가에 있어서 높은 점수를 받음으로써 기능성이나 맛에 있어서 긍정적인 결과를 얻었다. 그러므로 맥문동을 음료나 약선 등에 활용할 때에 맥문동의 전처리는 건조만하여 추출하기 보다는 찌거나 술을 도포하여 찐 다음 건조하여 볶는 방법이 적합한 것으로 생각된다.

Keywords

References

  1. 김우정, 구경형 (2001) 식품관능검사법. 효일출판사, 서울. pp 95-119.
  2. 김창민, 신민교, 안덕균, 이경순 (1997) 중약대사전 I. The encyclopedia of oriental herbal medicine. 도서출판 정담, 서울. pp 1262-1269.
  3. 박종철 (2002) 기능성 식품의 천연물 과학. 도서출판 효일, 서울. pp 52-53.
  4. 葉定江, 張世新 主編(1999) 중약포제학 (中藥炮製學). 인민위생출판사, 북경. pp 313-315.
  5. 식품공전 (2012) 식품공전 2. 한국식품산업협회, 서울. p 3.
  6. 식품의약품안전처 (2013) 식품공전. 식품의약품안전처, 청원. p 2-1-6.
  7. 한국생약학교수협의회 건강기능성식품 교재편찬위원회 (2008) 건강기능성식품. 도서출판 동명사, 파주. pp 408-409.
  8. 허준 (2001) 精校 東醫寶鑑. 도서출판 한미의학, 서울. p 1173.
  9. Bae KM, Park SH, Jung KH, Kim MJ, Hong SH, Song YO, Lee HS (2010) Effects of roasting conditions on physicochemical properties and sensory properties of Liriopis tuber. J Korean Soc Food Sci Nutr 39: 1503-1508. https://doi.org/10.3746/jkfn.2010.39.10.1503
  10. Baek NI, Cho SJ, Bang MH, Lee IJ, Park CG, Kim MS, Kim KS, Sung JD (1998) Cytotoxicity of steroid-saponins from the tuber of Liriope platyphylla W.T. J Korean Soc Agric Chem Biotech 41: 390-394.
  11. Cho KM, Joo OS (2012) Enhances antioxidant effect of purple sweet potato by roasting. Korean J Food Preserv 19: 735-743. https://doi.org/10.11002/kjfp.2012.19.5.735
  12. Choi WS (2012) Development of functional beverage using yam (Dioscorea opposita Thunb.). Food Industry and Nutrition 17: 20-22.
  13. Dubois M, Gilles KA, Hamilton JK, Revers PA, Smith F (1956) Colorimetric method for determination of sugar and related substance. Anal Chem 28: 350-352. https://doi.org/10.1021/ac60111a017
  14. Ha TY, Chun HS, Lee C, Kim YH, Han O (1999) Changes in physicochemical properties of steamed rice for soonneung during roasting. Korean J Food Sci Technol 31: 171-175.
  15. Hong MJ, Lee GD, Kim HK, Kwon JH (1998) Changes in functional and sensory properties of chicory roots induced by roasting processes. Korean J Food Sci Technol 30: 413-418.
  16. Jung MH, Lee SS, Park SH, Hwang HJ (2013) The antioxidative effect of ethanol extracts from Lithospermum erythrorhizon Siebold & Zucc., Xanthium strumarium Linn and Lonicera japonica. J Life Sci 23: 643-649. https://doi.org/10.5352/JLS.2013.23.5.643
  17. Jung MH, Yoon JM, Lee TH (2003) Effect of Liriopis tuber on the change of corticosterone in mice induced by starvation stress. Korean J Herbology 18: 279-287.
  18. Kato H, Lee IE, Cheyen NV, Kim SB, Hayse F (1987) Inhibition of nitrosamine formation by nondialyzable melanoidins. Agric Biol Chem 51: 1333-1339. https://doi.org/10.1271/bbb1961.51.1333
  19. Kim DS, Ahn BW, Yeum DM, Kim SB, Park YH (1987) Degradation of carcinogenic nitrosamine formation factor by natural food components. 1. Nitrite scavenging effect of vegetable extracts. Bull Korean Fish Soc 20: 463-468.
  20. Kim SD, Ku YS, Lee IZ, Kim MK, Park IK (2000) Major chemical components in fermented beverages of Liriopis tuber. J East Asian Soc Dietary Life 10: 281-287.
  21. Kim SD, Ku YS, Lee IZ, Kim ID, Youn KS (2001) General components and sensory evaluation of hot water extract from Liriopis tuber. J Korean Soc Food Sci Nutr 30: 20-24.
  22. Lee GD, Kim JO, Son JH, Kim HY (2012) Monitoring on physicochemical properties of Liriope platyphylla by the use of four dimentional response surface. Korean J Food Preserv 19: 560-568. https://doi.org/10.11002/kjfp.2012.19.4.560
  23. Lee KS, Kim GH, Seong BJ, Kim HH, Song MR, Kim MR, Lee GH (2010) Physiochemical characteristics of ginseng jungkwa produced with hot-water extract from maegmundong (Liriope platyphylla tubers). J Korean Soc Food Sci Nutr 39: 1819-1825. https://doi.org/10.3746/jkfn.2010.39.12.1819
  24. Lee KS, Kim GH, Kim HH, Choi JW, Lee HC, Song MR, Kim MR, Lee GH (2009a) Physiochemical characteristics of Liriope platyphylla tubers by drying process. J Korean Soc Food Sci Nutr 38: 1104-1110. https://doi.org/10.3746/jkfn.2009.38.8.1104
  25. Lee SH, Lee YR, Hwang IG, Woo KS, Kim KH, Kim KJ, Jeong HS (2009b) Antioxidant activities and quality characteristics of germinated rough rice tea according to roasting temperature, time and leaching condition. J Food Sci Technol 41: 386-391.
  26. Lee SK, Park JH, Kim YT (2009c) A study on the antioxidation and antimicrobial effect of megmoondong (Liriope platyphylla Wang et Tang) water extracts. Korean J Food & Nutr 22: 279-285.
  27. Lee YK, Lee MY, Kim SD (2004) Quality characteristics and dietary effect of baguette bread added with water extracts of Liriopis tuber on the blood glucose and serum cholesterol in diabetes induced rats. J East Asian Soc Dietary Life 14: 275-282.
  28. Lee YT, Seog HM, Kim SS, Kim KT, Hong HD (1994) Changes in physicochemical characteristics of immature barley kernels during roasting. Korean J Food Sci Technol 26: 336-342.
  29. Marcocci L, Packer L, Droy-Lefaix MT, Sekaki A, Gardes-Albert M (1994) Antioxidant action of Ginko biloba extract Egb 761. Methods Enzymol 234: 462-475. https://doi.org/10.1016/0076-6879(94)34117-6
  30. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428. https://doi.org/10.1021/ac60147a030
  31. Naik GH, Priyadarsini KI, Naik DB, Gangabhagirathi R, Mohan H (2004) Studies on the aqueous extract of terminaliachebula as a potent antioxidant and a probable radioprotector. Phytomedicine 20: 530-538.
  32. Park JH, Geon DG (2003) Pharmacognostical studies on the chines crude drug megmoondong. Korean J Pharmacogn 34: 6-9.
  33. Park SD, Lee GH, Lee YS, Kwon YK, Park JH, Choi SM, Shin SW (2007) Comparison of immunomodualtory effects of water-extracted Adenophorae radix, Liriopis tuber, Dendrobii Herba, Polygonati odorati Rhizoma and Polygonati Rhizoma. Korean J Oriental & Pathology 21: 414-424.
  34. Park SJ, Yoo SM, Kim YE (2012) Nutritional characteristics and screening of biological activity of cultured wild ginseng roots. Korean J Food & Nutr 25: 729-736. https://doi.org/10.9799/ksfan.2012.25.4.729
  35. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26: 1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  36. Rhee IJ, An JY (2003) Hepatoprotective effects of water extract of Liriopis tuber on carbon tetrachloride-induced hepatotoxicity in rats. Korean J Pharmacogn 34: 166-171.
  37. Roh SS, Choi HS, Kim DH, Seo YB (2008) Studies of anti-inflammation of Liriopis tuber to autoimmune diabetes in NOD mice. Korean J Oriental & Pathology 22: 766-770.
  38. Ryu KC, Chung HW, Kim KT, Kwon JH (1997) Opimization of roasting condition for high quality Polygonatum odoratum tea. Korean J Food Sci Technol 29: 776-783.
  39. Seo SJ, Kim NW (2010) Physiological activities of leaf and root extracts from Liriope platyphylla. Korean J Food Preserv 17: 123-130.
  40. Seong JD, Park YJ, Kim HY, Suh HS, Han KS (1995) Changes of tuber yield and total sugar content by different harvesting dates in Liriope platyphylla Wand et Tag. Korean J Medicinal Crop Sci 3: 56-60.
  41. Shin JS (2002) Saponin composition of Liriope platyphylla and Ophiopogon japonicus. Korean J Crop Sci 47: 236-239.
  42. Shin MJ, Ahn MS (2000) A study on the antioxidant activity of products of caramel-type-browning reaction. Korean J Soc Food Sci 16: 629-639.
  43. Song JH, Kang MG, Kim NM, Lee JS (2011) Nutritional and physiological functionalities of Liriope tuber (Cheongsim and Liriope tuber No.1). Korean J Medicinal Crop Sci 19: 478-483. https://doi.org/10.7783/KJMCS.2011.19.6.478
  44. Song SB, Ko JY, Kim JI, Lee JS, Jung TW, Kim KY, Kwak DY, Oh IS, Woo KS (2013) Changes in physicochemical characteristics and antioxidant activity of adzuki bean and adzuki bean tea depending on the variety and roasting time. J Food Sci Technol 45: 317-324.
  45. Song YB, Choi JS, Lee JE, Noh JS (2010) The antioxidant effect of hot water extract from the dried radish (Raphanus sativus L.) with pressurized roasting. J Korean Soc Food Sci Nutr 39: 1179-1186. https://doi.org/10.3746/jkfn.2010.39.8.1179