DOI QR코드

DOI QR Code

The Study on Anti-obesity Effects of Mulberry Leaves Contained Herbal Mixture

상엽(桑葉) 함유 한약복합제 추출물의 항비만(抗肥滿)효과 연구

  • Park, Jong Ik (Department of Pediatrics, College of Oriental Medicine, Wonkwang University) ;
  • Kang, Kyung Ha (Department of Pediatrics, College of Oriental Medicine, Wonkwang University) ;
  • Park, Eun Jung (Department of Pediatrics, College of Oriental Medicine, Wonkwang University)
  • 박종익 (원광대학교 한의과대학원 소아과교실) ;
  • 강경하 (원광대학교 한의과대학원 소아과교실) ;
  • 박은정 (원광대학교 한의과대학원 소아과교실)
  • Received : 2013.10.25
  • Accepted : 2013.11.14
  • Published : 2013.11.30

Abstract

Objectives This experimental study was designed to investigate the effects of Mulberry leaves contained herbal mixture (MLHM) on body weight, serum lipid level and adipocyte differentiation in high fat diet-fed obese mice. Methods Four-week old mice (wild-type C57/BL6) were used for all experiments. Cells were incubated with MLHM at the indicated concentration (0.04-4mg/ml) for 24h, and growth rate was assessed by MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. 3T3-L1 preadipocytes were incubated in DMEM for 2 days with the indicated concentrations of MLHM, and on Day 6, the cells were fixed and the cellular lipid contents were assessed by Oil-Red-O staining. The expression of peroxisome proliferator-activated receptor ${\gamma}$ (PPAR ${\gamma}$) and cytidine-cytidine-adenosine-adenosine-thymine (CCAAT)/enhancer-binding proteins ${\alpha}$ (C/EBP ${\alpha}$) as adipocyte-specific proteins were determined by real time RT-PCR and western blotting. In addition, body weight gain and serum lipid levels were measured in the mice with obesity induced by the high fat-diet for four weeks. Results Though MLHM did not show toxicity even at the concentration of 4mg/ml, MLHM significantly inhibited the differentiation of 3T3-L1 preadipocites in a dose-dependent manner. Also, MLHM significantly reduced the expressions of PPAR ${\gamma}$ and C/EBP ${\alpha}$ in a dose-dependent manner. Furthermore, MLHM significantly reduced body weight gain and LDL-cholesterol contents in high fat diet-fed obese mice. Conclusions These results demonstrate that MLHM exerts anti-obesity effect in 3T3-L1 cells and mice with obesity by high-fat diet.

Keywords

References

  1. Department of Internal Medicine, Seoul National University College of Medicine. Internal Medicine. Seoul: Koonja publishing inc. 1996:852-62.
  2. Georgy A Bray. Office management of obesity. Seoul: Hanmi medical Pub. 2005:21, 107, 114.
  3. Jung SH. Research study about Obesity-related information acquisition method. J Oreintal Rehab Med. 1998;8(2): 1-15.
  4. Kim TH, Yoo K. Obesity and tatty Liver disease. Korean Journal of Medicine. 2005;68(2):347-9.
  5. Ministry of Health and Welfare. Korea National Health and Nutrition Examination Survey (KNHANES V-1). Korea Centers for Disease Control and Prevention. Seoul, Korea:Korea Health Statistics. 2010.
  6. Oh KW, Jang MJ, Lee NY, Moon JS, Lee CG, Yoo MH, et al. Prevalence and trends in obesity among Korean children and adolescents in 1997 and 2005. Korean J Pediatr. 2008;51:950-5. https://doi.org/10.3345/kjp.2008.51.9.950
  7. Peter GK. Obesity as a medical problem. Nature. 2000;404(6778):635-43. https://doi.org/10.1038/35007508
  8. TCM assessment study. TCM Sympton differentiation Diagnostics. Beijing:People's Health Publishing House. 1987:43.
  9. Ko SC, Jeong KM. The Study about the Comparison of Oriental-Western Medicine on Childhood Obesity. Journal Korean Oriental Pediatrics. 1989;3(1):57-61.
  10. Obesity Study Group of Korean Primary Care Association. Theory and Practice of Obesity Studies. seoul: hankookbook. 1996:267.
  11. Park YW. Evidence-based prescriptions of functional foods fot weight control. Korean J Fam Med. 2003;24(5): 409-15.
  12. Park CY. The Update of Anti-Obesity Drug. Kyunghee medical book. 2002;18(2):77-85.
  13. The korean academy of oriental rehabilitation medicine. Oriental Rehabilitation Medicine. seoul:Koonja publishing inc. 2005:349-62, 384-96.
  14. Jung SH, Lee KH. Antidiabetic effect and mechanism of Mori folium on streoptozotocin-induced diabetic mouse. East-west pharmaceutical research institute college of pharmacy kyunghee university. 2000;28:87-99.
  15. Kim MS, Choue RW, Chung SH, Koo SJ. Blood glucose lowering effects of Mulberry leaves and silkworm extracts on mice fed with high- carbohydrate diet. The Korean journal of nutrition. 1998;31:117-25.
  16. Myo CS. coloured medicinal plant of Korea. 1St ed. Seoul:Academic Co. 1989.
  17. Jun YJ, Son MY, Khil MJ, Sung JS, Jeong JC, Kim DI. Cell differentiation and anti-oxydative effect of Dioscoreae Rhizoma on HeLa Cell. The Journal of oriental obstetrics & gynecology. 2007;20(2):139-54.
  18. Nam JY, Roh JJ, Seung JH, Son MY, Khil MJ, Sung JS, Kim DI. Non-toxic and Anti-oxydative effect of Dioscoreae Rhizoma on PC12 Cell. The Journal of oriental obstetrics &gynecology. 2006;19(4):61-76.
  19. McAnuff MA, Harding WW, Omoruyi FO, Jacobs H, Morrison EY, Asemota HN. Hypoglycemic effects of steroidal sapogenins isolated from jamaican bitter yam, Dioscorea poygonoides. Food Chem. Toxicol. 2005;43: 1667-72. https://doi.org/10.1016/j.fct.2005.05.008
  20. Kwon CS, Sohn HY, Kim SH, Kim JH, Son KH, Lee JS, Lim JK, Kim JS. Anti-obesity effect of Dioscorea nipponica Makino with lipasseinhibitory activity in rodents. Biosci. Biotechnol. Biochem. 2003;67:1451-6. https://doi.org/10.1271/bbb.67.1451
  21. Chen HL, Wang CH, Chang CT, Wang TC. Effects of Taiwanese yam (Dioscoreajaponica Thunb var. pseudojaponica Yamamoto) on upper gut function and lipid metabolism in Balb/c mice. Nutrition. 2003;19: 646-51. https://doi.org/10.1016/S0899-9007(03)00058-3
  22. Seo KI, Lee SW, Yang KH. Antimicrobial and antioxidative activities of Corni fructus extracts. Journal of the Korean society of post-harvest science & technology of agricultural products. 1999;6(1):99-103.
  23. Kim OK. Antidiabetic and antioxidative effects of Corni fructus in Streptozotocin-induced diabetic rats. Journal of the Korean oil chemists society. 2005;22(2):157-67.
  24. Sagi-Eisenberg R, Foreman J, Shelly R. Histamine release induced by histone and phorbol ester from rat peritoneal mast cells. Eur J Pharmacol. 1985;113:11-7. https://doi.org/10.1016/0014-2999(85)90337-1
  25. Kim SJ, Park SK. Antioxidative and Anti-inflammatory Effects of Aurantii Fructus Immaturus Pharmacopuncture. Korean Journal of Acupuncture. 2010;27(2):13-24.
  26. Seoul National University College of Medicine. Endocrinology jeonjeongpan. seoul:Seoul National University Press. 2006: 425-45, 491-5.
  27. Min HK. Clinical Endocrinology. seoul:Korea medical book. 1990:476-9, 482-6.
  28. Gordon Edlin, Eric Golanty. Health and Wellness. Yeong Mun pub. 2010:128.
  29. Kim YS. Causes and classification of obesity. Kyunghee medicalbook. 1995;11(3-4):244.
  30. Statistics Korea. Annual report on the cause of death statistics. 2000-10.
  31. Lee BK. Symptom differentiation treatment . Seongbosa. 1991:77.
  32. Korean Intellectual Property Office. 2008.
  33. Shin MK. Clinical Herbology. Yeonglimsa. 1986:171, 243, 384-5, 531.
  34. Kimura T, Nakagawa K, Kubota H, Kojima Y, Goto Y, Yamagishi K, Oita S, Oikawa S, Miyazawa T. Food-grade mulberry powder enriched with 1- deoxynojirimycin suppresses the elevation of postprandial blood glucose in humans. J Agric Food Chem. 2007;55(14):5869-74. https://doi.org/10.1021/jf062680g
  35. Park JM, Bong HY, Jeong HI, Kim YK, Kim JY, Kwon O. Postprandial hypoglycemic effect of mulberry leaf in Goto-Kakizaki rats and counterpart control Wistarrats. Nutr Res Pract. 2009;3(4):272-8. https://doi.org/10.4162/nrp.2009.3.4.272
  36. Oh KS, Ryu SY, Lee S, Seo HW, Oh BK, Kim YS, Lee BH. Melanin-concentrating hormone-1 receptor antagonism and anti-obesity effects of ethanolic extract from Morus alba leaves in diet-induced obese mice. J Ethnopharmacol. 2009;122(2):216-20. https://doi.org/10.1016/j.jep.2009.01.020
  37. Hsu JH, Wu YC, Liu IM, Cheng JT. Dioscorea as the principal herb of Die-Huang-Wan, a widely used herbal mixture in China, for improvement of insulin resistance in fructose-rich chow-fed rats. J Ethnopharmacol. 2007; 112(3):577-84. https://doi.org/10.1016/j.jep.2007.05.013
  38. Kim SW. The antioxidant activity of dioscoreae rhizoma on GC-1 spg cell. Graduate school of Kyung Hee University. 2006.
  39. Yamabe N, Kang KS, Matsuo Y, Tanaka T, Yokozawa T. Identification of antidiabetic effect of iridoid glycosides and low molecular weight polyphenol fractions of Corni Fructus, a constituent of Hachimi-jio-gan, in streptozotocininduced diabetic rats. Biol Pharm Bull. 2007;30(7): 1289-96. https://doi.org/10.1248/bpb.30.1289
  40. Elzbieta M, Kurowska, John A, Manthey. Hypolipidemic Effects and Absorption of Citrus Polymethoxylated Flavones in Hamsters with Diet- Induced Hypercholesterolemia. J. Agric. Food Chem. 2004;52: 2879-86. https://doi.org/10.1021/jf035354z
  41. Jung UJ, Lee MK, Jeong KS, Choi MS. The Hypoglycemic Effects of Hesperidin and Naringin Are Partly Mediated by Hepatic Glucose-Regulating Enzymes in C57BL/KsJdb/db Mice, J Nutr. 2004;134:2499-503.
  42. Jung UJ, Lee MK, Park YB, Kang MA, Choi MS. Effect of citrus flavonoids on lipid metabolism and glucose-regulating enzyme mRNA levels in type-2 diabetic mice. The International Journal of Biochemistry & Cell Biolog. 2006;38(7):1134-45. https://doi.org/10.1016/j.biocel.2005.12.002
  43. MacDougald OA, Hwang C, Fan H, Lane MD. Regulated expression of the obese gene product (leptin) in white adipose tissue and 3T3-L1 adipocytes. Preceedings of the National Academy of Sciences. 1995;92(20):9034-7. https://doi.org/10.1073/pnas.92.20.9034
  44. Chen, Yu-Kuo Cheung, Connie Reuhl, KennethR. Liu, AnnaBa Lee, Mao-Jung Lu, Yao-Ping Yang, ChungS. Effects of green tea polyphenol (-)-Epigallocatechin-3-gallate on newly developed high-fat/Western-style dietinduced obesity and metabolic syndrome in mice. Journal of agricultural and food chemistry. 2011;59(21):11862-71. https://doi.org/10.1021/jf2029016
  45. James M, Ntambi, Kim YC. Adipocyte differentiation and gene expression. J Nutr. 2000;130:3122-6.
  46. Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM. Transcriptional regulation of adipogenesis. Genes & Dev. 2000;14:1293-307.
  47. Jeon T, Hwang SG, Hirai S, Matsui T, Yano H, Kawda T, Lim BU, Ki D. Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci. 2004;75:3195-203. https://doi.org/10.1016/j.lfs.2004.06.012
  48. Cowherd RM, Lyle RE, McGehee Jr RE. Molecular regulation of adipocyte differentiation. Semin Cell Dev Biol. 1999;10:3-10. https://doi.org/10.1006/scdb.1998.0276
  49. Cornelius P, MacDougald OA, Lane MD. Regulation of adipocyte development. Annu Rev Nutr. 1994;14:99-129. https://doi.org/10.1146/annurev.nu.14.070194.000531
  50. Kim SH, Park HS, Lee MS, Cho YJ, Kim YS, Hwang JT, Sung MJ, Kim MS, Kwon DY. Vitisin A inhibits adipocyte differentiation through cell cycle arrest in 3T3-L1 cells. Biochem Biophys Res Commun. 2008; 372:108-13. https://doi.org/10.1016/j.bbrc.2008.04.188
  51. Kopelman PG. Obesity as a medical problem. Nature. 2000;404:635-43. https://doi.org/10.1038/35007508
  52. Camp HS, Ren D, Leff T. Adipogenesis and fat-cell function in obesity and diabetes. Trends in Molecular Medicine. 2002;8(9):442-7. https://doi.org/10.1016/S1471-4914(02)02396-1
  53. Rosen ED, MS Bruce. Molecular regulation of adipogenesis. Annu Rev Cell Dev Biol. 2000;16:145-71. https://doi.org/10.1146/annurev.cellbio.16.1.145
  54. Hwang CS, Lotus TM, Mandrup S, Lane MD. Adipocyte differentiation and leptin expression. Annual review of cell and developmental biology. 1997;13:231-59. https://doi.org/10.1146/annurev.cellbio.13.1.231
  55. Plaa GL. Detection and evaluation of chemically induced liver injury. In Principle and Methods of Toxicology. Philadelphia, PA, USA:Hayes AW, ed. Taylor & Francis. 1994:839-70.
  56. Gordon T, Castelli W, Dawber T. Lipoprotein, cardiovascular disease and death, the Framingham study. Arch Inter Med. 1981;14:1128-35.
  57. Applebaum BD, Haffner SM, Hartsook E, Luk KH, Albers JJ, Hazzard WR. Down-regulation of the low density lipoprotein receptor by dietary cholesterol. Am J Clin Nurt. 1984;39:360-7.
  58. Potter SM, Bakhit RM, Essexsorlie DL, Weingartner KE, Chapman KM, Nelson RA, Prabhudesai M, Savage WD, Nelson AI, Winter LW. Depression of plasma cholesterol in men by consumption of baked products containing soy protein. Am J Clin Nutr. 1993;58:501-6.

Cited by

  1. 동물실험에서 한약제재 경구투여가 비만에 미치는 영향에 대한 국내 연구보고 고찰 - 2010년 이후 발표된 논문을 중심으로 vol.27, pp.3, 2013, https://doi.org/10.18325/jkmr.2017.27.3.13
  2. Comparison with various mulberry leaves' and fruit's extract in lipid accumulation inhibitory effect at adipocyte model vol.35, pp.1, 2013, https://doi.org/10.7852/ijie.2017.35.1.1