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Bioethanol Production from Seaweed Gelidium amansii for Separated Hydrolysis and Fermentation (SHF)

해조류 우뭇가사리 (Gelidium amansii)의 분리당화발효를 이용한 바이오에탄올의 생산

  • Received : 2013.06.24
  • Accepted : 2013.09.25
  • Published : 2013.10.30

Abstract

The seaweed, Gelidium amansii, was fermented to produce bioethanol. Optimal pretreatment condition was determined as 94 mM $H_2SO_4$ and 8% (w/v) seaweed slurry at $121^{\circ}C$ for 60 min. The mono sugars of 40.4 g/L with 67% of conversion from total carbohydrate of 60.6 g/L with 80 g dw/L G. amansii slurry were obtained by thermal acid hydrolysis pretreatment and enzymatic saccharification. G. amansii hydrolysate was used as the substrate for ethanol production by Kluyveromyces marxianus KCTC 7150 and Candida tropicalis KCTC 7212 using 5L fermentor. The ethanol productions by K. marxianus KCTC 7150 and C. tropicalis KCTC 7212 were 17.8 g/L with $Y_{EtOH}$ of 0.48 at 120 h and 19.3 g/L with $Y_{EtOH}$ of 0.50 at 120 h, respectively.

Keywords

References

  1. Roesijadi, G., S. B. Jones, L. J. Snowden-Swan, and Y. Zhu (2010) Macroalgae as a Biomass Feedstock: A Preliminary Analysis, PNNL 19944. Pacific Northwest National Laboratory, Washington, USA.
  2. Ostgaard, K., M. Indergaard, S. Markussen, S. H. Knutsen, and A. Jensen (1993) Carbohydrate degradation and methane production during fermentation of Laminaria saccharina (Laminariales, Phaeophyceae). J. Appl. Phycol. 5: 333-342. https://doi.org/10.1007/BF02186236
  3. Jeong, G. T. and D. H. Park (2011) Production of levulinic acid from marine algae Codium fragile using acid-hydrolysis and response surface methodology. KSBB Journal 26: 341-346. https://doi.org/10.7841/ksbbj.2011.26.4.341
  4. Kim, M. J. and S. K. Kim (2012) Ethanol production by separate hydrolysis and fermentation and simultaneous saccharification and fermentation using Saccharina japonica. KSBB Journal 27: 86-90. https://doi.org/10.7841/ksbbj.2012.27.2.086
  5. Adams, J. M., J. A. Gallagher, and I. S. Donnison (2009) Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments. J. Appl. Phycol. 21: 569-574. https://doi.org/10.1007/s10811-008-9384-7
  6. Agbor, V. B., N. Cicek, R. Sparling, A. Berlin, and D. B. Levin (2011) Biomass pretreatment: Fundamentals toward application. Biotechnol. Advan. 29: 675-685. https://doi.org/10.1016/j.biotechadv.2011.05.005
  7. Boopathy, R., H. Bokang, and L. Daniels (1993) Biotransformation of furfural and 5-hydroxymethylfurfural by enteric bacteria. J. Ind. Microbiol. 11: 147-150. https://doi.org/10.1007/BF01583715
  8. Modig, T., G. Liden, and M. J. Taherzadeh (2002) Inhibition effects of furfural on alcohol dehydrogenase, aldehyde dehydrogenase and pyruvate dehydrogenase. Biochem. J. 363: 769-776. https://doi.org/10.1042/0264-6021:3630769
  9. AOAC (Association of Official Analytical Chemists) (1995) In: Cunniff, P. (Ed), Official Methods of Analysis of the Association of Official Analytical Chemists, 16th edition. Association of Officail Analytical Chemists, Arlington, VA.
  10. Sanchez-Machado, D. I., J. Lopez-Cervantes, P. Paseiro-Losada, and J. Lopez-Hernandez (2004) Fatty acids, total lipid, protein and ash contents of processed edible seaweeds. Food Chem. 85: 439-444. https://doi.org/10.1016/j.foodchem.2003.08.001
  11. Cho, Y. K., H. J. Kim, and S. K. Kim (2013) Bioethanol production from brown seaweed, Undaria pinnatifida, using NaCl acclimated yeast. Bioprocess Biosyst Eng. 36: 713-719. https://doi.org/10.1007/s00449-013-0895-5
  12. Rouhollah , H., N. Iraj, E. Giti, and A. Sorah (2007) Mixed sugar fermentation by Pichia stipitis, Saccharomyces cerevisiae, and an isolated xylose-fermenting Kluyveromyces marxianus and their cocultures. Afr. J. Biotechnol. 6(9): 1110-1114.
  13. Yuan, W. J., X. Q. Zhao, X. M. Ge, and F. W. Bai (2008) Ethanol fermentation with Kluyveromyces marxianus from Jerusalem artichoke grown in salina and irrigated with a mixture of seawater and freshwater. J. Appl. Microbiol. 105: 2076-2083. https://doi.org/10.1111/j.1365-2672.2008.03903.x

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