폴리머 (Polymer(Korea))

  • 제37권6호
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  • Pages.669-676
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  • 2013
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  • 0379-153X(pISSN)
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  • 2234-8077(eISSN)
한국고분자학회 (The Polymer Society of Korea)
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DOI QR코드

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In vivo 상에서 탈미네랄화된 골분이 함유된 PLGA 지지체를 이용한 추간판 디스크 재생

Regeneration of Intervertebral Disc Using Poly(lactic-co-glycolic acid) Scaffolds Included Demineralized Bone Particle In Vivo

  • 장지은 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 김혜윤 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 송정은 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 이동원 (전북대학교 BIN 융합공학과, 고분자나노공학과) ;
  • 권순용 (가톨릭대학교 의과대학 여의도성모병원 정형외과) ;
  • 정진화 (가톨릭대학교 의과대학 여의도성모병원 정형외과) ;
  • 강길선 (전북대학교 BIN 융합공학과, 고분자나노공학과)
  • Jang, Ji Eun (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National Univ.) ;
  • Kim, Hye Yoon (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National Univ.) ;
  • Song, Jeong Eun (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National Univ.) ;
  • Lee, Dongwon (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National Univ.) ;
  • Kwon, Soon Yong (Dept. of Orthopedic Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea) ;
  • Chung, Jin Wha (Dept. of Orthopedic Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea) ;
  • Khang, Gilson (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National Univ.)
  • 투고 : 2013.05.06
  • 심사 : 2013.06.12
  • 발행 : 2013.11.25
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초록

탈미네랄화된 골분(demineralized bone particle, DBP)은 연골 형성의 유도인자로 사용되기 때문에 조직공학에서 널리 사용되는 생체재료이다. 본 연구에서는 in vivo 환경에서 디스크 재생 효과를 연구하기 위해 DBP를 poly(lactic-co-glycolic acid) (PLGA)에 첨가하여 다공성 지지체를 제조하였다. 디스크의 섬유륜 조직을 반으로 절개한 후, 수핵 조직을 제거하여 디스크 결손을 유발시켰다. 빈 공간에 PLGA, DBP/PLGA 지지체를 이식하여 in vivo 환경에서 조직공학적 디스크 재생을 관찰하였다. 1, 2 및 3개월 후 디스크를 적출하여 글리코스아미노글라이칸(glycosaminoglycan, sGAG) 및 콜라겐 합성 정도를 측정하였으며 조직학적 평가로 H&E, Safranin-O, Alcian blue 염색과 면역조직학적 평가로 제 I형 콜라겐, 제 II형 콜라겐 염색을 수행하였다. 그 결과 DBP/PLGA 지지체에서 sGAG 및 콜라겐 함량이 높은 것을 확인하였으며 추간판 디스크 재생의 가능성을 확인하였다.

Demineralized bone particle (DBP) is a biomaterial used widely in the field of tissue engineering. In this study, in order to study the effect of DBP/poly(lactic-co-glycolic acid) (PLGA) scaffold on disc regeneration in vivo environment, we prepared the porous DBP/PLGA hybrid scaffold. Disc defect was induced by removing the nucleus pulposus tissue after incision the annulus fibrosus tissue in half and scaffolds were transplanted. After 1, 2 and 3 months later, the extracted discs were confirmed by collagen synthesis and glycosaminoglycan (sGAG). We conducted histology (H&E, Safranin-O, Alcian blue, Type I Collagen, Type II Collagen). From the results, it was confirmed that collagen and sGAG content were high in DBP/PLGA scaffold, and the regeneration of intervertebral disc was possible.

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피인용 문헌

  1. The host response to naturally-derived extracellular matrix biomaterials vol.29, pp.None, 2013, https://doi.org/10.1016/j.smim.2017.01.002