The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Antibacterial Effect of Ipyo-san against Methicillin-Resistant Staphylococcus aureus

입효산(立效散)의 Methicillin-Resistant Staphylococcus aureus에 대한 항균활성에 관한 연구

  • Yoon, Jae-Hong (Dept. of Oriental Internal Medicine, Dong-Guk University) ;
  • Choi, Yeun-Ju (Dept. of Oriental Internal Medicine, Dong-Guk University) ;
  • Jeong, Seung-Hyun (Dept. of Oriental Internal Medicine, Dong-Guk University) ;
  • Shin, Gil-Cho (Dept. of Oriental Internal Medicine, Dong-Guk University)
  • 윤재홍 (동국대학교 한의과대학 내과학교실) ;
  • 최연주 (동국대학교 한의과대학 내과학교실) ;
  • 정승현 (동국대학교 한의과대학 내과학교실) ;
  • 신길조 (동국대학교 한의과대학 내과학교실)
  • Published : 2013.09.30
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Abstract

Objectives : Methicillin-resistant Staphylococcus aureus (MRSA) has a cephalosporin and beta-lactam antibiotic-resistant strains. MRSA is one of the major pathogens causing hospital infection and the isolation ratio of MRSA has gradually increased. Consequently, increased resistance to antibiotics is causing serious problems in the world. Therefore, there is a need to develop alternative antimicrobial drugs for the treatment of infectious diseases. Methods : The antibacterial activities of Ipyo-san were evaluated against 2 strains of MRSA and 1 standard Methicillin-susceptible staphylococcus aureus (MSSA) strain by using the disc diffusion method, minimal inhibitory concentrations (MIC) assay, colorimetric assay using MTT test, checkerboard dilution test and time-kill assay performed under dark. Results : The MIC of Ipyo-san water extract against S. aureus strains ranged from 1000 to $2,000{\mu}g/ml$, so we confirmed that it had a strong antibacterial effect. Also, the combinations of Ipyo-san water extract and conventional antibiotics exhibited improved inhibition of MRSA with synergy effect. We suggest that Ipyo-san water extract against MRSA has antibacterial activity so it has potential as alternatives to antibiotic agents. For the combination test, we used Triton X-100 (TX) and DCCD for measurement of membrane permeability and inhibitor of ATPase. As a result, antimicrobial activity of Ipyo-san water extract was affected by the cell membrane. Conclusions : We suggest that the Ipyo-san water extract lead the treatment of bacterial infection to solve the resistance and remaining side-effect problems that are the major weak points of traditional antibiotics.

Keywords

References

  1. １. 김장수. 임상미생물학. 서울: 대한의학서적; 2003, p. 38-42.
  2. Fridkin SK, Hageman JC, Morrison M, Sanza LT, Como-sabetti K, Jernigan JA, et al. Methicillin -Resistant Staphylococcus aureus Disease in Three Communities. N Engl J Med 2005;352 :1436-44. https://doi.org/10.1056/NEJMoa043252
  3. 성인화. 黃芩과 黃柏 추출물의 MRSA와 Candida 에 대한 항균활성. 대한미생물학회지 2004;40(1) :17-22.
  4. 김기배, 이형탁, 구경회, 홍진우, 조수인. 黃連과 구성 생화합물의 약리작용에 대한 고찰. 대한한의학회지 2012;33(3):16-183.
  5. Kim KJ, Yu HH, Jeong SI, Cha JD, Kim SM, You YO. Inhibitory effect of Caesalpinia sappan on growth and invasion of methicillin -resistant Staphylococcus aureus. J Ethnopharmacol 2004;91(1):81-7. https://doi.org/10.1016/j.jep.2003.11.017
  6. 염대열, 백동기, 송용선. 小陷胸湯의 Methicillin 내성 Staphylococcus aureus에 대한 항균활성 연구. 동의생리병리학회지 2012;26(6):886-93.
  7. 許浚. 東醫寶鑑 -內景篇-. 1판. 서울: 신우문화사; 2003, p. 606.
  8. 박경철, 배기상, 최선복, 조일주, 곽태신, 이금산, 등. 급성췌장염 마우스 모델에서 지실과 지각 추출물의 보호효과. 대한본초학회지 2012;27(5) :9-14.
  9. 엄용대, 김대한, 정종길, 신민교, 송호준. 알레르기반응에 대한 枳實과 枳殼의 효과 비교 연구. 대한한의학회지 2001;22(4):10-21.
  10. 김성진, 박상균. 지각 약침액의 항산화 및 항염증 효과에 관한 연구. 경락경혈학회지 2010;27(2) :13-24.
  11. 김대준, 노성수. 吳茱萸 물 추출물이 급성역류성 식도염에 미치는 효과. 대한본초학회지 2012; 27(1):51-8.
  12. 윤현정, 허숙경, 이영태, 박원환, 박선동. 마우스 대식세포 및 사람 혈관 내피세포에서 오수유 (Evodia officinalis DODE) 메탄올 추출물의 항염증 효과. 대한본초학회지 2008;23(1):29-38.
  13. Lee YS, Kang OH, Choi JG, Oh YC, Chae HS, Kim JH, et al. Synergistic effects of the combination of galangin with gentamicin against methicillinresistant Staphylococcus aureus. J Microbiol 2008;46:283-8. https://doi.org/10.1007/s12275-008-0012-7
  14. Kwon DY, Kang OH, Choi JG, Lee YS, Oh YC, Chae HS, et al. Antibacterial effect of Dryopteris crassirhizoma against methicillinresistant Staphylococcus aureus. Fitoterapia 2007;78:430-3. https://doi.org/10.1016/j.fitote.2007.03.026
  15. Veljic M, Tarbuk M, Marin PD, Ciric A, Sokovic M, Marin M. Antimicrobial activity of methanol extracts of mosses from Serbia. Pharm Biol 2008;46:871-5. https://doi.org/10.1080/13880200802367502
  16. Noble WC, Virani Z, Cree RG. Co-transfer of vancomycin and other resistance genes from Enterococcus faecalis NCTC 12201 to Staphylococcus aureus. FEMS Microbiol Lett 1992;72:195-8.
  17. Nascimento AM, Brandão MG, Oliveira GB, Fortes IC, Chartone-Souza E. Synergistic bactericidal activity of Eremanthus erythropappus oil or beta-bisabolene with ampicillin against Staphylococcus aureus. Antonie Van Leeuwenheok 2007;92(1):95-100. https://doi.org/10.1007/s10482-006-9139-x
  18. Linnett PE, Beechey RB. Inhibitors of the ATP synthethase system. Methods Enzymol 1979;55:472-518. https://doi.org/10.1016/0076-6879(79)55061-7
  19. Blot SI, Vandewoude KH, Hoste EA, Colardyn FA. Outcome and attributable mortality in critically ill patients with bacteremia involving methicillin-susceptible and methicillin-resistant Staphylococcus aureus. Arch Intern Med 2002; 162(19):2229-35. https://doi.org/10.1001/archinte.162.19.2229
  20. Cosgrove SE, Sakoulas G, Perencevich EN, Schwaber MJ, Karchmer AW, Carmeli Y. Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta -analysis. Clin Infect Dis 2003;36(1):53-9. https://doi.org/10.1086/345476
  21. Chang FY, MacDonald BB, Peacock JE Jr, Musher DM, Triplett P, Mylotte JM, et al. A prospective multicenter study of Staphylococcus aureus bacteremia: incidence of endocarditis, risk factors for mortality, and clinical impact of methicillin resistance. Medicine Baltimore 2003;82(5):322-32. https://doi.org/10.1097/01.md.0000091185.93122.40
  22. 이경인, 최철희, 김선민, 표병식. MRSA에 대한 울금 추출 및 분획물의 항균활성과 항생제 증강 효과. 생약학회지 2010;41(1):38-42.
  23. 전국한의과대학 본초학교수. 本草學. 서울: 영림사; 1998, p. 180-1, 337-8, 350-1.
  24. 吳普. 神農本草經. 초판. 서울: 의성당; 2003, p. 79-80, 195-8.
  25. Odds FC. Synergy, antagonism, and what the chequerboard puts between them. J Antimicro Chemo 2003;52(1):1. https://doi.org/10.1093/jac/dkg301
  26. Guadalupe MN, Blanca EL, Mariano VP, Fortino SS. In vitro activity effects of combinations of cephalothin, dicloxacillin, imipenem, vancomycin and amikacin against methicillin-resistant Staphylococcus spp. strains. Ann Clin Microbiol Antimicrob 2006;5:25. https://doi.org/10.1186/1476-0711-5-25
  27. Junji S, Hitoshi K, Motoyuki S, Koji O, Katsuyuki K, Nobuo N, et al. Effects of various types of Triton X on the susceptibilities of methicillin -resistant staphylococci to oxacillin. FEMS Microbiol Lett 1997;153(2):327-31. https://doi.org/10.1111/j.1574-6968.1997.tb12592.x
  28. Hitoshi K, Junji S, Motoyuki S, Yoichiro M, Hidekazu S. The effect of Triton X-100 on the in-vitro susceptibility of methicillin-resistant Staphylococcus aureus to oxacillin. J Antimicrob Chemother 1994;34(6):885-97. https://doi.org/10.1093/jac/34.6.885
  29. Jung HJ, Lee DG. Synergistic antibacterial effect between silybin and N,N'-dicyclohexylcarbodiimide in clinical Pseudomonas aeruginosa isolates. J Microbiol 2008;46(4):462-7. https://doi.org/10.1007/s12275-008-0138-7
  30. 신인식, 강옥화, 정대기, 강희정, 김지은, 황형칠, 등. 神效越桃散이 메티실린에 내성이 있는 Staphylococcus aureus에 대한 항균활성에 관한 연구. 대한본초학회지 2013;28(1):59-64.