Antibacterial Effect of Ipyo-san against Methicillin-Resistant Staphylococcus aureus

입효산(立效散)의 Methicillin-Resistant Staphylococcus aureus에 대한 항균활성에 관한 연구

  • Yoon, Jae-Hong (Dept. of Oriental Internal Medicine, Dong-Guk University) ;
  • Choi, Yeun-Ju (Dept. of Oriental Internal Medicine, Dong-Guk University) ;
  • Jeong, Seung-Hyun (Dept. of Oriental Internal Medicine, Dong-Guk University) ;
  • Shin, Gil-Cho (Dept. of Oriental Internal Medicine, Dong-Guk University)
  • 윤재홍 (동국대학교 한의과대학 내과학교실) ;
  • 최연주 (동국대학교 한의과대학 내과학교실) ;
  • 정승현 (동국대학교 한의과대학 내과학교실) ;
  • 신길조 (동국대학교 한의과대학 내과학교실)
  • Published : 2013.09.30

Abstract

Objectives : Methicillin-resistant Staphylococcus aureus (MRSA) has a cephalosporin and beta-lactam antibiotic-resistant strains. MRSA is one of the major pathogens causing hospital infection and the isolation ratio of MRSA has gradually increased. Consequently, increased resistance to antibiotics is causing serious problems in the world. Therefore, there is a need to develop alternative antimicrobial drugs for the treatment of infectious diseases. Methods : The antibacterial activities of Ipyo-san were evaluated against 2 strains of MRSA and 1 standard Methicillin-susceptible staphylococcus aureus (MSSA) strain by using the disc diffusion method, minimal inhibitory concentrations (MIC) assay, colorimetric assay using MTT test, checkerboard dilution test and time-kill assay performed under dark. Results : The MIC of Ipyo-san water extract against S. aureus strains ranged from 1000 to $2,000{\mu}g/ml$, so we confirmed that it had a strong antibacterial effect. Also, the combinations of Ipyo-san water extract and conventional antibiotics exhibited improved inhibition of MRSA with synergy effect. We suggest that Ipyo-san water extract against MRSA has antibacterial activity so it has potential as alternatives to antibiotic agents. For the combination test, we used Triton X-100 (TX) and DCCD for measurement of membrane permeability and inhibitor of ATPase. As a result, antimicrobial activity of Ipyo-san water extract was affected by the cell membrane. Conclusions : We suggest that the Ipyo-san water extract lead the treatment of bacterial infection to solve the resistance and remaining side-effect problems that are the major weak points of traditional antibiotics.

Keywords

References

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