Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Increasement of antioxidative activity in Codonopsis lanceolata adventitious root treated by Methyl jasmonate and salicylic acid

Methyl Jasmonate 및 Salicylic Acid 처리에 의한 더덕(Codonopsis lanceolata) 부정근의 항산화 활성 증가

  • Hwang, Hyun-Jung (Department Food and Nutrition, Silla University) ;
  • Song, Gwanpill (Jeju Environment Research Institute, Rm 603, Bl Center, Jeju Tourism College) ;
  • Kim, Mi-Hyang (Department Food and Nutrition, Silla University) ;
  • Do, Seon-Gil (Univera, Inc.) ;
  • Bae, Kee-Hwa (Hongcheon Institute of Medicinal Herb, National Institute of Biological Resources)
  • 황현정 (신라대학교 식품영양학과) ;
  • 송관필 ((주)제주자연환경연구소) ;
  • 김미향 (신라대학교 식품영양학과) ;
  • 도선길 ((주)유니베라) ;
  • 배기화 ((재)홍천메디칼허브연구소, 국립생물자원관)
  • Received : 2013.08.13
  • Accepted : 2013.08.28
  • Published : 2013.09.30
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Abstract

Traditionally, Codonopsis lanceolata root have been used as a source of natural heath food. This study was initiated to investigate the impacts of methyl jasmonate (MeJA) and salicylic acid (SA) on adventitious growth C. lanceolata, the production of secondary metabolites, such as flavonoids, total phenolic compound, antioxidative activity (DPPH). The highest phenolics content was observed in treatment of 20 uM MeJA (74.53 mg/g). The content of total flavonoids followed the similar pattern as that of total phenolics, showing 38.45 mg/g of C. lanceolata treated by 20 uM MeJA. The DPPH scavenging activity was 24.2 ($IC_{50}$) of C. lanceolata treated by 20 uM MeJA. These results provide useful information for enhancing biological properties of cultural roots of C. lanceolata.

본 연구의 목적은 더덕부정근 배양에서 MeJA 및 SA의 처리가 페놀화합물, 플라보노이드의 생산에 미치는 영향과 이러한 이차대사산물의 증가에 따른 더덕배양근의 항산화활성의 효과를 조사하기 위해 수행되었다. 다양한 농도의 MeJA 및 SA를 더덕배양근에 처리한 결과, 20 uM MeJA에서 총페놀 화합물의 생산은 74.53 mg/g으로 무처리구보다 2배 높은 함량을 보였다. 하지만, MeJA 및 SA의 처리는 부정근의 생장을 감소시키는 것으로 나타났다. 총플라보노이드의 함량 역시 20 uM MeJA의 처리구에서 38.45 mg/g으로 가장 높은 함량을 보였다. 20 uM의 MeJA 처리에 의한 총페놀화합물과 총플라보노이드 함량의 증가는 결과적으로 더덕부정근의 항산화활성을 $IC_{50}$ 값 수준에서 MeJA 무처리구에 비해 2배, 3년생 재배더덕에 비해서는 11배 이상 증가시키는 것으로 나타났다.

Keywords

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