Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Organic Solvent-tolerant Lipase from Pseudomonas sp. BCNU 154

Pseudomonas sp. BCNU 154 유래의 유기용매 내성 리파아제

  • Choi, Hye Jung (Department of Biology and Interdisciplinary Program for Biotechnology, Changwon National University) ;
  • Hwang, Min Jung (Department of Biology and Interdisciplinary Program for Biotechnology, Changwon National University) ;
  • Seo, Jeoung-Yoon (Department of Environmental Engineering, Changwon National University) ;
  • Joo, Woo Hong (Department of Biology and Interdisciplinary Program for Biotechnology, Changwon National University)
  • 최혜정 (창원대학교 생물학과.생물공학협동과정) ;
  • 황민정 (창원대학교 생물학과.생물공학협동과정) ;
  • 서정윤 (창원대학교 환경공학과) ;
  • 주우홍 (창원대학교 생물학과.생물공학협동과정)
  • Received : 2013.01.23
  • Accepted : 2013.10.22
  • Published : 2013.10.30
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Abstract

An organic solvent-tolerant lipase of Pseudomonas sp. BCNU 154 that was isolated from wastewater in the industrial complex region had optimal activity at $37^{\circ}C$ and pH 8. This crude extracellular lipase from BCNU 154 exhibited maximum stability in toluene, retaining about 6.01 U/ml (117.53%) activity for 2 h. $Ca^{2+}$, $Mg^{2+}$, $NH_4{^+}$, and $Na^+$ ions and triton X-100 activated the enzymes, whereas $Ba^{2+}$, $Hg^{2+}$, and $Zn^{2+}$ ions inhibited their activity. Pseudomonas sp. BCNU 154 lipase revealed stable activity comparable to that of the commercial immobilized Novozym 435. Thus, this organic solvent-tolerant lipase could have potential as a whole cell biocatalyst in industrial chemical processes without the use of immobilization.

산업공단지역의 폐수에서 분리한 유기용매 내성 Pseudomonas sp. BCNU 154 리파아제의 최적조건은 $37^{\circ}C$, pH8로 조사되었다. BCNU 154의 crude 리파아제는 toluene에서 2시간 반응시 효소활성 약 6.01 U/ml (117.53%)로 가장 안정한 것으로 나타났다. 한편 $Ca^{2+}$, $Mg^{2+}$, $NH_4{^+}$, $Na^+$ 이온 및 triton X-100은 효소를 활성화시킨 반면에 $Ba^{2+}$, $Hg^{2+}$ 및 $Zn^{2+}$ 이온은 효소활성을 억제하였다. Pseudomonas sp. BCNU 154 리파아제는 상용 고정화 효소인 Novozym 435와 비교해서도 안정한 활성을 보였다. 그러므로 유기용매 내성 리파아제는 별도의 고정화 처리없이도 화학산업공정에서 가능성 있는 whole cell 생물촉매로서 유용할 것으로 판단된다.

Keywords

References

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